Characterization of Collagen/Lipid Nanoparticle–Curcumin Cryostructurates for Wound Healing Applications

Abstract

NanoparticlesCurcumin-loaded collagen cryostructurates have been devised for wound healing applications. Curcumin displays strong antioxidant, antiseptic, and anti-inflammatory properties, while collagen is acknowledged for promoting cell adhesion, migration and differentiation. However, when curcumin is loaded directly into collagen hydrogels, it forms large molecular aggregates and clogs the matrix pores. A double-encapsulation strategy is therefore developed by loading curcumin into lipid nanoparticles (LNP), and embedding these particles inside collagen scaffolds. The resulting collagen/LNP cryostructurates have an optimal fibrous structure with ≈100 µm average pore size for sustaining cell migration. Results show that collagen is structurally unaltered and that nanoparticles are homogeneously distributed amidst collagen fibers. Hydrogels soaked in saline buffer release about 20 to 30% of their nanoparticles content within 24 h, while achieved 100% release after 25 days. When exposed to NIH 3T3 fibroblasts, these hydrogels provide a satisfactory scaffold for cell interaction as early as 4 h after seeding, with no cytotoxic counter effect. These positive features make the collagen/lipid cryostructurates a promising material for further use in wound healing.

Functional Biomaterials for Treatment of Chronic Wound

“…Colbased hydrogels have the advantages of high water absorption, good biocompatibility, and low antigenicity. Col also enhances granulation tissue and neovascularization by eliminating high concentrations of MMPs (Simpson et al, 2007;Laghezza Masci et al, 2019). Alginate is an anionic polysaccharide that can form hydrogels under very mild conditions and in the absence of organic solvents (Lee and Mooney, 2012).…”

Versatile role of curcumin and its derivatives in lung cancer therapy

“…A variety of studies have been performed to enhance the bioavailability of curcumin and some of them, particularly nanostrategies have been successful. Different nanocarriers such as liposomes (Ternullo, Schulte Werning, Holsaeter, & Skalko‐Basnet, 2019), polymeric nanoparticles (Okagu, Verma, McClements, & Udenigwe, 2020), carbon dots (Pal, Mohiyuddin, & Packirisamy, 2018), and lipid nanoparticles (Laghezza Masci et al, 2019) are able to dramatically promote the bioavailability of curcumin, thereby enhancing its therapeutic capability.…”

Multilamellar ceramide core-structured microvehicles with substantial skin barrier function recovery

“…20,21 Such nanofibrillary materials with high aspect ratios are featured to form a tight and dense fibrillary membrane at the lipid–water interface owing to their amphiphilic nature. 21–23 These findings suggest that properly selecting lipids and surfactants with a well-adjusted composition is critical for stabilizing suspended lipid particles.…”