Characterization of cold responsive uncoupling protein1 (UCP1) gene from Brassica juncea L. (Czern. and Coss.)

Lakhanpal, Neha; Verma, Deepika; Kaur, Rajveer; Singh, Kashmir

doi:10.1007/s13562-017-0421-y

Cited by 2 publications

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The number of introns and exons is same as that of previously reported EXPA1 gene from different plant species (Harmer et al 2002). High level of gene sequence similarities was observed between related species and genera in other crops (Lakhanpal et al 2018). This showed the conserved nature of expansin genes in the closely related species or genera.…”

Section: Discussionsupporting

confidence: 75%

Isolation and characterization of water-deficit stress-responsive α-expansin 1 (EXPA1) gene from Saccharum complex

et al. 2019

View full text Add to dashboard Cite

In this study, full-length (1282-1330 bp) α-expansin 1 (EXPA1) gene from three different accessions belonging to Saccharum complex (Saccharum officinarum-SoEXPA1, Erianthus arundinaceus-EaEXPA1, and Saccharum spp. hybrid-ShEXPA1) was isolated using RAGE technique and characterized. The intronic and coding regions of isolated expansin genes ranged between 526-568 and 756-762 bp, respectively. An open reading frame encoding a polypeptide of 252 amino acids was obtained from S. officinarum and commercial sugarcane hybrid, whereas 254 amino acids were obtained in E. arundinaceus, a wild relative of Saccharum. Bioinformatics analysis of deduced protein revealed the presence of specific signature sequences and conserved amino acid residues crucial for the functioning of the protein. The predicted physicochemical characterization showed that the protein is stable in nature with instability index (II) value less than 40 and also clearly shown the dominance of random coil in the protein structure. Phylogenetic analysis revealed high conservation of EXPA1 among Saccharum complex and related crop species, Sorghum bicolor and Zea mays. The docking study of EXPA1 protein showed the interaction with xylose, which is present in xyloglucan of plant cell wall, elucidated the role of the expansin proteins in plant cell wall modification. This was further supported by the subcellular localization experiment in which it is clearly seen that the expansin protein localizes in the cell wall. Relative expression analysis of EXPA1 gene in Saccharum complex during drought stress showed high expression of the EaEXPA1 in comparison with SoEXPA1 and ShEXPA1 indicating possible role of EaEXPA1 in increased water-deficit stress tolerance in E. arundinaceus. These results suggest the potential use of EXPA1 for increasing the water-deficient stress tolerance levels in crop plants.

show abstract

Section: Discussionsupporting

confidence: 75%

Isolation and characterization of water-deficit stress-responsive α-expansin 1 (EXPA1) gene from Saccharum complex

et al. 2019

View full text Add to dashboard Cite

show abstract

The uncoupling of respiration in plant mitochondria: keeping reactive oxygen and nitrogen species under control

Popov

Syromyatnikov

Fernie

et al. 2020

Journal of Experimental Botany

View full text Add to dashboard Cite

Plant mitochondrial respiration involves operation of various alternate pathways. These pathways participate, both directly and indirectly, in the maintenance of mitochondrial functions though they do not contribute to energy production being uncoupled from the generation of an electrochemical gradient across the mitochondrial membrane and thus from ATP production. Recent findings suggest that uncoupled respiration is involved in reactive oxygen species (ROS) and nitric oxide (NO) scavenging, regulation and homeostasis. Here we discuss specific roles and possible functions of the uncoupled mitochondrial respiration in ROS and NO metabolism. The mechanisms of expression and regulation of the NDA, NDB and NDC type non-coupled NADH and NADPH dehydrogenases, the alternative oxidase (AOX), and the uncoupling protein (UCP) are examined in relation to their involvement in the establishment of the stable far-from-equilibrium state of plant metabolism. The role of uncoupled respiration in controlling the levels of ROS and NO as well as inducing signaling events is considered. Secondary functions of uncoupled respiration include its role in protection from stress factors and roles in biosynthesis and catabolism. It is concluded that uncoupled mitochondrial respiration plays an important role in providing rapid adaptation of plants to changing environmental factors via regulation of ROS and NO.

show abstract

Characterization of cold responsive uncoupling protein1 (UCP1) gene from Brassica juncea L. (Czern. and Coss.)

Cited by 2 publications

References 54 publications

Isolation and characterization of water-deficit stress-responsive α-expansin 1 (EXPA1) gene from Saccharum complex

Isolation and characterization of water-deficit stress-responsive α-expansin 1 (EXPA1) gene from Saccharum complex

The uncoupling of respiration in plant mitochondria: keeping reactive oxygen and nitrogen species under control

Contact Info

Product

Resources

About