Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India

Rahman, Mafruza S; Sharma, Rajeev; Borah, Probodh; Chakraborty, Apurba; Devi, Mandakini R. K; Longjam, Neeta

doi:10.1590/s1678-91992012000100010

Cited by 14 publications

(4 citation statements)

References 6 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is in accordance with those reported in previous studies on wild birds in Sicily, in which the most commonly reported species were E. coli , C. freundii, and Klebsiella oxytoca [ 25 ]. In addition, C. perfringens , which, after E. coli, was the most isolated species from both birds and mammals, was indicated as a possible part of the microbiota in wild animals by some authors, although more studies on its role in the wild are needed [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Study on Bacteria Isolates and Antimicrobial Resistance in Wildlife in Sicily, Southern Italy

et al. 2021

View full text Add to dashboard Cite

Wild environments and wildlife can be reservoirs of pathogens and antibiotic resistance. Various studies have reported the presence of zoonotic bacteria, resistant strains, and genetic elements that determine antibiotic resistance in wild animals, especially near urban centers or agricultural and zootechnical activities. The purpose of this study was the analysis, by cultural and molecular methods, of bacteria isolated from wild animals in Sicily, Italy, regarding their susceptibility profile to antibiotics and the presence of antibiotic resistance genes. Bacteriological analyses were conducted on 368 wild animals, leading to the isolation of 222 bacterial strains identified by biochemical tests and 16S rRNA sequencing. The most isolated species was Escherichia coli, followed by Clostridium perfringens and Citrobacter freundii. Antibiograms and the determination of resistance genes showed a reduced spread of bacteria carrying antibiotic resistance among wild animals in Sicily. However, since several wild animals are becoming increasingly close to residential areas, it is important to monitor their health status and to perform microbiological analyses following a One Health approach.

show abstract

Section: Discussionmentioning

confidence: 99%

Study on Bacteria Isolates and Antimicrobial Resistance in Wildlife in Sicily, Southern Italy

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Moreover, Twedt (1993) [ 33 ] also reported that the strains of C. perfringens that produce the toxins can be primarily detected via diarrhea symptoms and feces of the infected host. C. perfringens shows 30–60% infection among various species of animals and birds [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Toxicogenetic Profiling of Clostridium perfringens in Buffalo and Cattle: An Update from Pakistan

Khan

Humza

Yang

et al. 2021

Toxins

View full text Add to dashboard Cite

Clostridium perfringens is a Gram-positive bacterium that possess seven toxinotypes (A, B, C, D, E, F, and G) that are responsible for the production of six major toxins, i.e., α, β, ε, ι, CPE, and NetB. The aim of this study is to find out the occurrence of toxinotypes in buffalo and cattle of Punjab province in Pakistan and their corresponding toxin-encoding genes from the isolated toxinotypes. To accomplish this aim, six districts in Punjab province were selected (i.e., Lahore, Sahiwal, Cheecha Watni, Bhakkar, Dera Ghazi Khan, and Bahawalpur) and a total of 240 buffalo and 240 cattle were selected for the collection of samples. From isolation and molecular analysis (16S rRNA), it was observed that out of seven toxinotypes (A-G), two toxinotypes (A and D) were found at most, whereas other toxinotypes, i.e. B, C, E, F, and G, were not found. The most frequently occurring toxinotype was type A (buffalo: 149/240; cattle: 157/240) whereas type D (buffalo: 8/240 cattle: 7/240) was found to occur the least. Genes encoding toxinotypes A and D were cpa and etx, respectively, whereas genes encoding other toxinotypes were not observed. The occurrence of isolated toxinotypes was studied using response surface methodology, which suggested a considerable occurrence of the isolated toxinotypes (A and D) in both buffalo and cattle. Association between type A and type D was found to be significant among the isolated toxinotypes in both buffalo and cattle (P < 0.05). Correlation was also found to be positive and significant between type A and type D. C. perfringens exhibits a range of toxinotypes that can be diagnosed via genotyping, which is more reliable than classical toxinotyping.

show abstract

“…Regarding pigeons, scarce studies reported the prevalence of C. perfringens ; for instance, Rahman, Sharma [ 41 ] reported an isolation rate of 33.3%. Despite the high isolation rate in pigeon intestine, no NE lesions were observed.…”

Section: Discussionmentioning

confidence: 99%

Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

Ahmed

Bayomi

Hamed³

et al. 2022

Veterinary Sciences

View full text Add to dashboard Cite

In this study, we determined the prevalence and toxin types of antibiotic-resistant Clostridium perfringens in chicken, pigeons, camels, and humans. We investigated the inhibitory effects of AgNPs on biofilm formation ability of the isolates and the genetic relatedness of the isolates from various sources determined using RAPD-PCR. Fifty isolates were identified using PCR, and all the isolates were of type A. The cpe and cpb2 genes were detected in 12% and 56% of the isolates, respectively. The effect of AgNPs on biofilm production of six representative isolates indicated that at the highest concentration of AgNPs (100 µg/mL), the inhibition percentages were 80.8–82.8%. The RAPD-PCR patterns of the 50 C. perfringens isolates from various sources revealed 33 profiles and four clusters, and the discriminatory power of RAPD-PCR was high. Multidrug-resistant C. perfringens isolates are predominant in the study area. The inhibition of biofilm formation by C. perfringens isolates was dose-dependent, and RAPD-PCR is a promising method for studying the genetic relatedness between the isolates from various sources. This is the first report of AgNPs’ anti-biofilm activity against C. perfringens from chickens, pigeons, camels, and humans, to the best of our knowledge.

show abstract

Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India

Cited by 14 publications

References 6 publications

Study on Bacteria Isolates and Antimicrobial Resistance in Wildlife in Sicily, Southern Italy

Study on Bacteria Isolates and Antimicrobial Resistance in Wildlife in Sicily, Southern Italy

Occurrence and Toxicogenetic Profiling of Clostridium perfringens in Buffalo and Cattle: An Update from Pakistan

Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

Contact Info

Product

Resources

About