Characterization of Citrus Cultivars and Clones in Cyprus Through Microsatellite and RAPD Analysis

Hvarleva, T.; Kapari-Isaia, T.; Papayiannis, L. C.; Atanassov, A.; Hadjinicoli, A.; Kyriakou, A.

doi:10.1080/13102818.2008.10817554

Cited by 24 publications

(21 citation statements)

References 26 publications

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“…Indeed, the use of four loci SSR in 51 accessions of Citrus gave a total of 18 alleles with an average of 4.5 alleles per locus. Heterozygous deficiency was estimated for all the 20 cultivars studied (Hobs = 0.671; Hexp = 0.707; Fis = 0.051), which was consistent with the results previously reported by Hvarleva et al (2008). This result could be explained by the modality of reproduction by vegetative propagation (Bretó et al, 2001).…”

Section: Discussionsupporting

confidence: 82%

“…It is also important to note that the three loci, TAA15, TAA27, and CAC23 considered in this study were amplified in the Egyptian Citrus accessions belonging to eight different species with a high level of polymorphism (Hussein et al, 2003). In addition, the genetic diversity observed in this study was similar to that obtained by Hvarleva et al (2008) in cultivated local Citrus in Cyprus. Indeed, the use of four loci SSR in 51 accessions of Citrus gave a total of 18 alleles with an average of 4.5 alleles per locus.…”

Section: Discussionsupporting

confidence: 69%

“…Starting with the comparison of isozymes in the 1970s (Torres et al, 1978), researchers adopted restriction fragment length polymorphism (RFLP) and random amplification of polymorphic DNA (RAPD) in the 1980s and early 1990s. More recently, amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR and SSR), chloroplast DNA markers (cpSSR), and interretrotransposon amplified polymorphisms (IRAP) have been used to identify and categorize the Citrus varieties (Fang and Roose, 1997;Bretó et al, 2001;Sankar and Moore, 2001;Barkley et al, 2006;Deng et al, 2007;Pang et al, 2007;Hvarleva et al, 2008;Jannati et al, 2009). These studies revealed a high allelic diversity and an excess of heterozygotes for some groups of ancestral species, especially in oranges and grapefruit.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers

et al. 2016

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ABSTRACT. Citrus are one of the most cultivated crops in the world. Economically, they are very important fruit trees in Tunisia. Little is known about the genetic diversity of the Tunisian Citrus germplasm. Exploring this diversity is a prerequisite for the identification and characterization of the local germplasm to circumvent and controlling genetic erosion caused by biotic and abiotic stress to aid its conservation and use. In the present study, we explored the genetic diversity of 20 Tunisian orange cultivars [Citrus sinensis (L.) Osbeck] and established their relationships by using seven simple sequence repeat (SSR) loci. In total, 37 alleles and 44 genotypes were scored. The sizes of alleles ranged from 90 to 280 bp. The number of alleles per locus was from 4 to 7, with an average of 5.28. Polymorphic information content value changed from 0.599 to 0.769 with an average of 0.675. Analysis of the genotypes revealed a heterozygote deficiency across all the genotypes. The observed heterozygosity varied from 0 to 1 (average of 0.671). Cluster analysis showed that three groups could be distinguished and the polymorphism occurred independently of the geographical origin of the studied orange cultivars. The detected SSR genotypes allowed the establishment of an identification key with a discriminating power of 100%. Multivariate analysis and the neighbor-joining phylogenetic tree indicated a narrow genetic base for the orange cultivars. The usefulness of SSR markers for orange fingerprinting and evaluation of the genetic diversity in the Tunisian germplasm are discussed in this paper.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers

et al. 2016

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“…It is indicated this result suggests that mutation occurred in at least one of them although horticultural traits are not known to between them (Fang &Roose, 1997). In other study, it was found identical microsatellite profiles at 9 out of 10 SSR loci among analyzed orange cultivars and clones (Hvarleva et al, 2008). For one locus 'Frost Valencia' and 'Shekeriko' acidless local Cyprus orange were discriminated from 'Shamouti', 'Jaffa', 'Valencia long' and 'Aematousiki' oranges.…”

Section: Genetic Diversity In Orangementioning

confidence: 77%

Genetic Diversity in Citrus

Uzun¹,

Yeşiloğlu²

2012

Genetic Diversity in Plants

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“…Four microsatellite loci have been suggested to be useful tools for DNA typing in sweet orange. In another study, 51 accessions representing 8 citrus species from the citrus collection have also been analyzed using 10 microsatellite and 6 RAPD markers (Hvarleva et al, 2008). SRAP markers have been used to evaluate the genetic diversity within 86 citrus and their relatives in Aurantioideae.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of sour orange (Citrus aurantium) accessions and their relatives using SSR and SRAP markers

Polat¹,

Kaçar

Yeşiloğlu³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Citrus production with its many varieties is of importance since it provides economically important products for Turkish exports. Sour orange is a rootstock commonly used for propagating the different scion varieties. Knowledge of the genetic diversity of the rootstock accessions would be useful in order to improve citrus breeding programs. We studied genetic relationships and diversity of 51 accessions of sour orange (Citrus aurantium) and their relatives using SSR (simple sequence repeat) and SRAP (sequence-related amplified polymorphism) molecular markers. Twenty-one SRAP primer combinations were tested on these accessions and relatives, producing 167 polymorphic fragments, with a mean of 8.0 and a mean polymorphism information content value of 0.47. Seventeen SSR primers also produced 30 polymorphic fragments, with a mean of 1.4 per primer and a mean polymorphism information content value of 0.39. The unweighted pair-group method with arithmetic average analysis using combined SSR and SRAP data showed a similarity range from 0.71 to 1.00 among the accessions. In the cluster analysis, sour orange relatives were indicated as a separate group from sour orange. 'Macrophylla' and 'Mexican lime' were the accessions most distinct (0.71) from the others. We conclude that genetic diversity in these sour orange accessions is lower and some of them were identical.

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Characterization of Citrus Cultivars and Clones in Cyprus Through Microsatellite and RAPD Analysis

Cited by 24 publications

References 26 publications

Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers

Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers

Genetic Diversity in Citrus

Molecular characterization of sour orange (Citrus aurantium) accessions and their relatives using SSR and SRAP markers

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