Characterization of Chromosome-Mediated BlaOXA-894 in Shewanella xiamenensis Isolated from Pig Wastewater

Zou, Huiyun; Zhou, Ziyu; Xia, Huiyu; Zhao, Qian; Li, Xuewen

doi:10.3390/ijerph16193768

Cited by 7 publications

(4 citation statements)

References 24 publications

(41 reference statements)

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“…The bla OXA-48-like in 111B and 111D were chromosomal mediated genes inserted between the LysR family transcriptional regulator and the C15 gene (Fig. 1a) similar to previously published S. xiamenensis sx20 which carried chromosomal mediated bla OXA-894 gene [25].…”

Section: Real-time Pcr Detection In Rectal Swab Samples and Phenotypisupporting

confidence: 84%

Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj

2021

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Background Shewanella xiamenensis has been reported in water environment and in patients and can act as the originator of oxacillinase in gram-negative bacteria. In order to assess genome plasticity and its functional properties related diarrhea symptoms in pilgrim, comparisons of draft genome sequences of the two isolates were conducted with other closely related genomes. Results We isolated S. xiamenensis 111B and 111D strains from a pilgrim before travels to the Hajj and during travels with diarrhea symptom, respectively. Whole-genome sequencing showed that draft genome size of 111B strain was 5,008,191 bp, containing 49 kb of a putative plasmid. The genome size of 111D was 4,964,295 bp containing 225 kb of a putative plasmid that shared the backbone sequences with the hospital wastewater strain T17. Comparatively, two Hajj strains are identical at 97.3% identity and 98.7% coverage. They are closely related to river water strain, AS58 by SNPs analysis. Notably, a novel blaOXA-48 allele blaOXA-547 was identified in 111D, sharing 99.5% identity with blaOXA-546 and blaOXA-894. Multiple copies of virulence specific genes, such as capsular polysaccharide biosynthesis, O-antigen and lasB (vibriolysin related gene) have been identified specifically in 111D, but absent in 111B strain. Conclusions The whole genome sequences of S. xiamenensis strain 111B and 111D, including comparative genomic analysis, highlight here the potential for virulence factors that might be related to the cause of diarrhea in humans and also indicate the possible acquisition of pathogenic bacteria, including antibiotic resistance genes or plasmids during the Hajj.

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Section: Real-time Pcr Detection In Rectal Swab Samples and Phenotypisupporting

confidence: 84%

Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj

2021

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“…Regarding the genera observed in each experimental group, it is important to note the role of Shewanella in iron respiration, a genus isolated from HHS group (as reported above). This process attracts much social and scientific attention due to its contribution to environmental pollution control and remediation field, promoting degradation of contaminants, such as aromatic compounds ( 76 , 77 ). It is interesting to mention that many disinfectants used at the farm contain aromatic compounds among their active ingredients, that might have facilitated the persistence of this genus in the house environment.…”

Section: Discussionmentioning

confidence: 99%

Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status

Marin,

Migura-García,

Rodríguez

et al. 2024

Front. Vet. Sci.

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IntroductionStringent regulations in pig farming, such as antibiotic control and the ban on certain additives and disinfectants, complicate disease control efforts. Despite the evolution of microbial communities inside the house environment, they maintain stability over the years, exhibiting characteristics specific to each type of production and, in some cases, unique to a particular company or farm production type. In addition, some infectious diseases are recurrent in specific farms, while other farms never present these diseases, suggesting a connection between the presence of these microorganisms in animals or their environment. Therefore, the aim of this study was to characterise environmental microbiomes of farms with high and low sanitary status, establishing the relationships between both, health status, environmental microbial ecology and its functionality.MethodsFor this purpose, 6 pig farms were environmentally sampled. Farms were affiliated with a production company that handle the majority of the pigs slaughtered in Spain. This study investigated the relationship among high health and low health status farms using high throughput 16S rRNA gene sequencing. In addition, to identify ecologically relevant functions and potential pathogens based on the 16S rRNA gene sequences obtained, functional Annotation with PROkaryotic TAXa (FAPROTAX) was performed.Results and DiscussionThis study reveals notable differences in microbial communities between farms with persistent health issues and those with good health outcomes, suggesting a need for protocols tailored to address specific challenges. The variation in microbial populations among farms underscores the need for specific and eco-friendly cleaning and disinfection protocols. These measures are key to enhancing the sustainability of livestock farming, ensuring safer products and boosting competitive edge in the market.

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“…The ambler class D b-lactamase OXA-48 can hydrolyze penicillins, imipenem, and extended spectrum cephalosporins that for the first time reported in carbapenemresistant K. pneumoniae isolate in Turkey [10]. The bla OXA has more than 800 variants [34].…”

Section: What Is Oxa?mentioning

confidence: 99%

Metallo-β-lactamase, extended spectrum β-lactamase and mcr-1 gene as major therapeutic challenges

Talebi

Hashemi

2020

Reviews in Medical Microbiology

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Antibiotic resistance is a major problem worldwide. There are different types of resistance specially in Gram-negative bacteria. Emergence of carbapenem resistance in Enterobacteriaceae, Pseudomonas, Acinetobacter and Klebsiella spp. is a main public health problem in the world. The b-lactamases are classified according to their functional similarities or molecular structures. There are two various families of b-lactamases that are found in bacteria. One of the causes of bacterial resistance is failure in treatment. Metallo-b-lactamases (MBLs) are associated with serine protease that are categorized in Ambler class B. MBL gene transfer is horizontally by mobile genetic elements. Extended spectrum b-lactamases are the main class of b-lactamases and show resistance to cephalosporins. In this review, we focus on special resistance genes and their origin. Also, we try to demonstrate new treatments for resistant bacteria.

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Characterization of Chromosome-Mediated BlaOXA-894 in Shewanella xiamenensis Isolated from Pig Wastewater

Cited by 7 publications

References 24 publications

Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj

Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj

Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status

Metallo-β-lactamase, extended spectrum β-lactamase and mcr-1 gene as major therapeutic challenges

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