2015
DOI: 10.1186/s12864-015-1523-3
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Characterization of chromosomal and megaplasmid partitioning loci in Thermus thermophilus HB27

Abstract: BackgroundIn low-copy-number plasmids, the partitioning loci (par) act to ensure proper plasmid segregation and copy number maintenance in the daughter cells. In many bacterial species, par gene homologues are encoded on the chromosome, but their function is much less understood. In the two-replicon, polyploid genome of the hyperthermophilic bacterium Thermus thermophilus, both the chromosome and the megaplasmid encode par gene homologues (parABc and parABm, respectively). The mode of partitioning of the two r… Show more

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Cited by 8 publications
(24 citation statements)
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“…The chromids and/or megaplasmids whose copy numbers have been examined in the family Rhizobiaceae (48)(49)(50) and the genera Burkholderia (51) have a copy number approximately equal to that of the chromosome. Similarly, the copy numbers of the large plasmids of Thermus thermophilus (52) and Sphingomonas wittichii (53) are similar to those of the chromosomes. However, the copy number of the chromid of the fast-replicating organism V. cholerae can actually be lower than that of the chromosome depending on the growth medium (54).…”
Section: Replication and Segregation Dynamics In Multipartite Genomesmentioning
confidence: 70%
“…The chromids and/or megaplasmids whose copy numbers have been examined in the family Rhizobiaceae (48)(49)(50) and the genera Burkholderia (51) have a copy number approximately equal to that of the chromosome. Similarly, the copy numbers of the large plasmids of Thermus thermophilus (52) and Sphingomonas wittichii (53) are similar to those of the chromosomes. However, the copy number of the chromid of the fast-replicating organism V. cholerae can actually be lower than that of the chromosome depending on the growth medium (54).…”
Section: Replication and Segregation Dynamics In Multipartite Genomesmentioning
confidence: 70%
“…Pseudomonas aeruginosa 9/4/TGTTCCACGtGGAACa half-parSs GTTCCAC or GTTTCAC [89][90][91] non-essential 2-4% in LB medium, to 7% in an M9 medium (wt < 0.01%) [92] Reduced growth rate, 10-15% increase in cell size and 10% longer generation time, altered colony morphology, affected motility; decreased ParA stability [92] Pseudomonas putida ? */3 TGTTCCACGTGGAACA [63] non-essential 5-10% in minimal medium during the transition from exponential to stationary phase [93] Defects in chromosome partitioning, abnormal cell morphologies during the deceleration phase of growth independent of the medium used [63,93] Vibrio cholerae chrI: 3/3/NGTTNCACGTGAAACN chrII: 10/9/NTTTACANTGTAAAN [94] non-essential chr1 essential chr2 no change in parB1 mutant [95] Increased frequency of replication initiation, disturbed ori positioning in cell poles [95], no segregation defect for V. cholerae chrI [94] Deinococci Deinococcus radiodurans chrI: 3/1/NGTTTcgcGtgaAACN [96] non-essential 8%-13% for ∆parB1, (wt >1%) [96] Reduced growth rate for ∆parB1 [96] Thermus thermophilus 1/1/TGTTTCCCGTGAAACA [97] non-essential 3% for ∆parAB (wt 1.2%) [97] No apparent growth defects for ∆parAB [97,98] Abbreviations: chrI/chrII-primary/secondary chromosome in the multipartite genome; wt-wild-type; ? * -only contig with P. putida oriC was analyzed for presence of parSs in the cited reference.…”
Section: Gammaproteobacteriamentioning
confidence: 99%
“…ParB nucleation around the parS sequence correlates with the formation of ParB foci in fluorescence microscopy analyses [76,92,97,117,[127][128][129][130][131] or the presence of wide peaks (up to 50 kbp), encompassing parS in chromatin immunoprecipitation analyses (ChIP-seq or ChIP-microarray), indicating the incorporation of parS proximal DNA in the ParB complex [68,71,72,79,80,89,90,129,132].…”
Section: The Structure Of the Parb-pars Complexmentioning
confidence: 99%
“…The real-time quantitative PCR was essentially performed based on the method described (Breuert et al 2006; Li et al 2015). Specifically, two loci (TTC1610 (near oriC ) and TTC0574 (closer to terC ) on the chromosome were chosen as the investigation targets.…”
Section: Methodsmentioning
confidence: 99%
“…The mutagenized pJ- pyrFE was then digested with Nde I to get rid of the pyrE gene, and ligated with a bleomycin resistance marker blm (sequence data from Brouns et al 2005). The plasmid pMK- parB-sgfp allowing expression of ParB-sGFP in T. thermophilus was constructed in a former study (Li et al 2015). The plasmids pUC-Δ mreB :: kat and pUC-Δ parA :: blm were gene deletion vectors for generation of double knockout mutant Δ mreB :: kat /Δ parA :: blm in T. thermophilus HB27.…”
Section: Methodsmentioning
confidence: 99%