Characterization of Chromosomal Aberrations in Human Gastric Carcinoma Cell Lines Using Chromosome Painting

Chun, Yong Hyuck; Kil, Joon Il; Suh, Young Suk; Kim, Seung Han; Kim, Hyun; Park, Sun Hwa

doi:10.1016/s0165-4608(99)00217-4

Cited by 16 publications

(17 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overexpression of GCIP can inhibit anchorage-independent growth of SW480 colon cancer cells in a soft agar colony formation assay. The role of GCIP to be a tumor suppressor is also supported by its genomic location in chromosome 15q14-15, a common region deleted or LOH in several types of human tumors, including the colorectal [Tomlinson et al, 1999;Park et al, 2000], breast [Wick et al, 1996;Gonzalez et al, 1999], mesothelioma [Balsara et al, 1999;De Rienzo et al, 2001], pancreatic [Rigaud et al, 2000], bladder [Natrajan et al, 2003], and gastric tumor [Chun et al, 2000].…”

Section: Discussionmentioning

confidence: 99%

GCIP/CCNDBP1, a helix–loop–helix protein, suppresses tumorigenesis

Stafford

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

Deletions and/or loss of heterozygosity (LOH) on chromosome 15 (15q15 and 15q21) have been found in several human tumors, including carcinomas of the colorectum, breast, lung, prostate, and bladder, suggesting the presence of potential tumor suppressor gene(s) in this particular region of chromosome 15. GCIP also called CCNDBP1, DIP1, or HHM, localized at chromosome 15q15, is a recently identified helix-loop-helix leucine zipper (HLH-ZIP) protein without a basic region like the Id family of proteins. In this study, we reported that the expression of GCIP was significantly downregulated in several different human tumors, including breast tumor, prostate tumor, and colon tumors. In human colon tumors, both mRNA and protein expression levels of GCIP were decreased significantly compared to the normal tissues. Treatment of colon cancer cells SW480 with sodium butyrate (NaB), which induces colon cancer cell differentiation, can induce the upregulation of GCIP expression, suggesting that the protein functions as a negative regulator in cell proliferation. Overexpression of GCIP in SW480 colon cancer cell line resulted in a significant inhibition on tumor cell colony formation, while silencing of GCIP expression by siRNA can promote cell colony formation. Furthermore, overexpression of GCIP inhibited the transcriptional activity of cyclin D1 promoter and the expression of cyclin D1 protein in the cell. Finally, we demonstrate that GCIP specifically interacts with one of the class III HDAC proteins, SirT6, which is important for maintaining genome stability. Together, our data suggest a possible function of GCIP in tumor suppression.

show abstract

Section: Discussionmentioning

confidence: 99%

GCIP/CCNDBP1, a helix–loop–helix protein, suppresses tumorigenesis

Stafford

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…11 Because this cell line is derived from a male patient, and has a normal X chromosome karyotype, a single allele for the miR-222»221 cluster exists in these cells. Thus, mutation of just one allele enabled us to obtain knockout cell lines.…”

Section: Nuclease-mediated Knockout Of Mir-221 and Mir-222mentioning

confidence: 99%

Knockout of miR-221 and miR-222 reveals common and specific targets for paralogous miRNAs

et al. 2017

View full text Add to dashboard Cite

MicroRNAs (miRNAs) regulate the expression of mRNA through sequence-specific binding of the 3 0 untranslated region (UTR). The seed sequence of miRNAs is the key determinant for target site recognition. Paralogous miRNAs, which share the same seed sequences but differ in their 3 0 regions, are known to regulate largely overlapping groups of mRNAs. However, no study has analyzed functional differences between paralogous miRNAs with proper experimental methods. In this study, we compared the targets of paralogous miRNAs, miR-221 and miR-222. Using a nuclease-mediated genome engineering technique, we established knockout cell lines for these miRNAs, and precisely analyzed differences in target regulation. We found that miR-221 and miR-222 suppress the previously identified targets, CDKN1B and CDKN1C, differentially. Whereas both miRNAs suppressed CDKN1B, only miR-221 suppressed CDKN1C. From transcriptome analyses, we found that several different target mRNAs were regulated by each of miR-221 and miR-222 independently, although a large number of mRNAs responded commonly to miR-221 and miR-222. This is the first study to compare the mRNA regulations by paralogous miRNAs and illustrate that paralogous miRNAs with the same seed sequence also have difference in target regulation.

show abstract

“…The ACP01 cell line described here is the first line of adenocarcinoma cells developed in Brazil. Cytogenetic studies of gastric neoplasia conducted in western nations are rare, mainly due to technical difficulties related to in vitro cell culture (6). Nevertheless, various chromosome alterations have been reported in gastric cancer, involving different chromosomes, such as chromosomes 3,5,6,8,12,13, and 17 (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

“…Gastric cancer cell lines have been used as a biological model to obtain information about the clonal evolution of this malignancy (6,10,11). Yet, in spite of the importance of gastric cancer, relatively few such cell lines are available (12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor

Lima

Rissino

Harada

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01 and characterize it cytogenetically by means of in vitro immortalization. Cells were transformed from an intestinal-type gastric adenocarcinoma (T4N2M0) originating from a 48-year-old male patient. This is the first gastric adenocarcinoma cell line established in Brazil. The most powerful application of the cell line ACP01 is in the assessment of cytotoxicity. Solid tumor cell lines from different origins have been treated with several conventional and investigational anticancer drugs. The ACP01 cell line is triploid, grows as a single, non-organized layer, similar to fibroblasts, with focus formation, heterogeneous division, and a cell cycle of approximately 40 h. Chromosome 8 trisomy, present in 60% of the cells, was the most frequent cytogenetic alteration. These data lead us to propose a multifactorial triggering of gastric cancer which evolves over multiple stages involving progressive genetic changes and clonal expansion.

show abstract

Characterization of Chromosomal Aberrations in Human Gastric Carcinoma Cell Lines Using Chromosome Painting

Cited by 16 publications

References 24 publications

GCIP/CCNDBP1, a helix–loop–helix protein, suppresses tumorigenesis

GCIP/CCNDBP1, a helix–loop–helix protein, suppresses tumorigenesis

Knockout of miR-221 and miR-222 reveals common and specific targets for paralogous miRNAs

Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor

Contact Info

Product

Resources

About