Characterization of chitosan–polycaprolactone blends for tissue engineering applications

Sarasam, Aparna R.; Madihally, Sundararajan V.

doi:10.1016/j.biomaterials.2005.01.071

Cited by 377 publications

(316 citation statements)

References 28 publications

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“…7,64 In a separate study, we blended antibacterial chitosan with PCL and showed that blending compromises the antibacterial property of the material. 113,114 Further, the blend membranes showed better support for fibroblast spreading and proliferation. Surface roughness analysis of blend membranes showed significant increase in roughness relative to chitosan membranes, and observed antibacterial activity could be partially attributed to changed topography.…”

Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 93%

Cell colonization in degradable 3D porous matrices

Lawrence

Madihally

2008

Cell Adhesion & Migration

173

146

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Cell colonization is an important in a wide variety of biological processes and applications including vascularization, wound healing, tissue engineering, stem cell differentiation and biosensors. During colonization porous 3D structures are used to support and guide the ingrowth of cells into the matrix. In this review, we summarize our understanding of various factors affecting cell colonization in three-dimensional environment. The structural, biological and degradation properties of the matrix all play key roles during colonization. Further, specific scaffold properties such as porosity, pore size, fiber thickness, topography and scaffold stiffness as well as important cell material interactions such as cell adhesion and mechanotransduction also influence colonization.

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Section: © 2 0 0 8 L a N D E S B I O S C I E N C E D O N O T D I S mentioning

confidence: 93%

Cell colonization in degradable 3D porous matrices

Lawrence

Madihally

2008

Cell Adhesion & Migration

173

146

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“…Chitosan has generated enormous interest as a biomaterial due to its various advantages, such as (1) low cost, (2) easy availability, (3) biocompatibility and (4) anti-microbial activity 6 . The potential of chitosan as a tissue-engineering scaffold is based on its cationic nature and high charge density in solution.…”

Section: Introductionmentioning

confidence: 99%

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

2015

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Chitosan is soluble in diluted acid solutions and can easily form films by casting. However, residual acid neutralization should be performed for biomedical applications what may compromise physical and mechanical properties of the films. Thus, plasticizers can be added to improve these properties. The aim of this study was to characterize morphological, barrier and mechanical properties, besides evaluate the in vitro cytotoxicity of sorbitol-plasticized and NaOH-Na 2 CO 3 neutralized chitosan membranes for skin substitute application. Scanning electron microscopy, X-ray diffraction, water vapor permeability and mechanical tests were carried out to characterize the obtained membranes. Moreover, Vero cells were used for in vitro cytotoxicity evaluation. In this paper, we report a non-cytotoxic sorbitol-plasticized chitosan membrane with desirable properties for skin substitution, such as flexibility, water vapor permeability and high percentage of elongation.

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“…Both biologically derived and synthetic materials have been extensively explored in regenerative medicine (Lutolf and Hubbell, 2005). In particular, synthetic polymeric materials, such as poly(lactic acid), poly(glycolic acid) and poly(lactide-co-glycolide), have received considerable attention, since their properties of biocompatibility and biodegradation make them attractive for therapeutic applications (Lee et al, 2006;Mano et al, 2004;Peter et al, 1998, Sarasam andMadihally, 2005). However, these materials exhibit insufficient mechanical strength to support bone regeneration.…”

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confidence: 99%

Biocompatibility and biodegradation of polyester and polyfumarate based-scaffolds for bone tissue engineering

Cortizo

Molinuevo

Cortizo

2008

J Tissue Eng Regen Med

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Biodegradable and biocompatible polymeric scaffolds have been recently introduced for tissue regeneration purpose. In the present study we aimed to develop polymeric-based scaffolds for bone regeneration. Two polyesters, poly-β-propiolactone (PBPL), poly-ε-caprolactone (PCPL) and two polyfumarates, polydiisopropyl fumarate (PDIPF), polydicyclohexyl fumarate (PDCF) were chosen to prepare films which can support osteoblastic growth. Scanning electron microscopy and water contact angle were used to characterize the matrices. Biodegradation studies were performed both in PBS buffer and using an in vitro macrophage degradation assay. Mouse calvaria-derived MC3T3E1 cells and UMR106 rat osteosarcoma cell lines were used to perform biocompatibility and cytotoxicity studies. The polyesters, the most hydrophilic polymers studied, showed a rougher and more porous surfaces than the polyfumarates. Under acellular conditions, only PBPL was degraded by hydrolytic mechanisms. However, macrophages performed an active degradation of all polymeric films. Osteoblasts developed well-defined actin fibres without evidence of cytotoxicity when growing on the films. The number of UMR106 osteoblasts that adhered to the PBPL-based film was higher than that of the cells attached to the PECL and polyfumarates (PDIPF and PDCF) matrices. Both UMR106 and MC3T3E1 osteoblastic lines showed protein levels comparable to control conditions, demonstrating that they grew well on all surfaces. However, UMR106 cells showed a significant increase in proliferation on polyester-derived scaffolds (PBPL and PECL). The alkaline phosphatase activity of UMR106, an osteoblastic marker, was significantly higher than that of control plastic dishes. MC3T3E1 cells expressed similar levels of this differentiation marker in all polymeric matrices. We found similar collagen protein content after 48 h culture of UMR106 cells on all surfaces. However, important differences were evident in the MC3T3E1 line. In conclusion, the synthetic polymeric-based scaffold we have developed and studied supports adhesion, growth and differentiation of two osteoblastic cell lines, suggesting that they could be useful in bone tissue regeneration.

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Characterization of chitosan–polycaprolactone blends for tissue engineering applications

Cited by 377 publications

References 28 publications

Cell colonization in degradable 3D porous matrices

Cell colonization in degradable 3D porous matrices

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

Biocompatibility and biodegradation of polyester and polyfumarate based-scaffolds for bone tissue engineering

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