Characterization of chemical constituents and rats metabolites of Shuanghua Baihe tablets by HPLC‐Q‐TOF‐MS/MS

Abstract

A high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry method was established to detect as many constituents in rat biological fluids as possible after oral administration of Shuanghua Baihe tablets (SBT). An Agilent Poroshell 120 EC-C18 column was adopted to separate the samples, and mass spectra were acquired in positive and negative modes. First, the fingerprints of SBT were established, resulting in 32 components being detected within 40 min. Among these compounds, 12 were tentatively identified by comparing the retention times and mass spectral data with those of reference standards and the reference literature; the other 20 components were tentatively assigned solely based on the MS data. Furthermore, metabolites in rat plasma and urine after oral administration of SBT were also analyzed. A total of 19 compounds were identified, including 13 prototypes and six metabolites through metabolic pathways of demethylation and glucuronide conjugation. Glucuronidated alkaloids were the main constituents in the plasma, and were then excreted from urine. This is the first systematic study on the metabolic profiling of SBT.

Identification of chemical constituents and rat metabolites of Kangxianling granule by HPLC‐Q‐TOF‐MS/MS

“…High‐performance liquid chromatography (HPLC) coupled with high‐resolution mass spectrometry such as quadrupole time‐of‐flight tandem mass spectrometry (Q‐TOF‐MS/MS) has been proven to be an efficient tool for the identification of the chemical constituents and metabolites in complex matrices (Ren et al ., ; Zhao et al ., ; Zheng et al ., ). Therefore it has become an effective analytical technique in traditional Chinese medicine research.…”

Simultaneous determination of 14 components in Shuanghua Baihe tablets by ultrasonic-assisted extraction and HPLC-MS/MS

“…SBT consists of ten crude herbs, including Coptidis Rhizoma, C. Bungeanae Herba, Isatidis Radix, Arnebiae Radix, Lonicerae Japonicae Flos, Lophatheri Herba, Rehmanniae Radix, Lilii bulbus, Asari radix et rhizome, and Snake Bile, and is mainly used for the treatment of recurrent oral ulcers. 14 Previous studies of SBT have mainly focused on the clinical application, [15][16][17][18][19][20][21] qualitation of the chemical constituents and metabolites in rats, 22 and the pharmacokinetic proles, 23,24 and only one article has reported on the content determination of chlorogenic acid. 25 Therefore, an investigation of the extraction technology and a simultaneous determination of the multiple targets in order to better control the quality of SBT are in urgent need.…”

Pharmacokinetics and safety of the multiple constituents of Shuanghua Baihe tablets in healthy subjects

“…16 In order to indicate the active ingredients in SBT and its mechanism of action and promote the internationalization and modernization of SBT, well studies are needed to be done. In our previous study, 12 the chemical compounds in SBT and constituents in rat plasma following oral administration of SBT were initially identied using liquid chromatography-high resolution mass spectrometry techniques. Besides, pharmacokinetic studies are useful to explain and predict a variety of events related to the efficacy and toxicity of drugs, thus it is valuable to perform pharmacokinetic studies for evaluating the rationality and compatibility of herbs or prescriptions.…”

“…As multiple compounds exist in herbal medicines, we should choose representative active compounds and be able to explain pharmacokinetic behaviors and drug-drug interactions in herbal medicines. 21 According to our previous study, 12 nine major bioactive alkaloids (Fig. 1; berberine, epiberberine, coptisine, palmatine, jatrorrhizine, magnoorine and berberrubine derived from Coptidis Rhizoma; corynoline and acetylcorynoline derived from Corydalis Bungeanae Herba) were selected as indicative compounds for the pharmacokinetic study.…”