Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates

Alshelmani, Mohamed Idris; Loh, Teck Chwen; Foo, Hooi Ling; Lau, Wei Hong; Sazili, Awis Qurni

doi:10.1155/2013/689235

Cited by 17 publications

(24 citation statements)

References 25 publications

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“…In comparison, the most active β-glucosidase activity responsible for reducing sugar liberation from cellobiose was exhibited between Day 4 and Day 6 of SSF. This finding was in agreement with Alshelmani et al [55,93], who reported that high CMCase, xylanase, and mannanase activity was exhibited by P. polymyxa , Bacillus megaterium, and Bacillus amyloliquefaciens during Day 4 of SSF of PKC.…”

Section: Discussionsupporting

confidence: 93%

“…In addition, LAB are generally known as “generally recognized as safe” (GRAS) and “quantified presumption of safety” (QPS) due to their beneficial probiotic characteristics [50,51]. The postbiotic metabolites produced by Lactobacillus plantarum strains, one of the major and important species of LAB that employed in this study, have been extensively reported as a versatile health supplement for rat and livestock as a growth promoter to produce safer and healthier meat for consumers [52,53,54,55]. Paenibacillus polymyxa and other LAB, such as Lactobacillus salivarius, could reduce the crude fiber and improved nutrient digestibility of broiler fed with fermented lignocellulosic biomass [56,57,58,59].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods

Foo

et al. 2019

IJMS

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Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-mannosidase, and β-mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods

Foo

et al. 2019

IJMS

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“…(2016) Bacillus subtilis ST-141, Saccharomycetes N5Protease, lipase, amylase, xylanaseStainless steel fermentation tank48 h at 30 °CWang et al. (2017)Palm kernel cake Aspergillus wentii Lipase, amylase, dextranase, cellulase, pectinase, β-glucosidase, mannanase, xylanase–48 h at 30 °CMuangkeow and Chinajariyawong (2009) Aspergillus wentii Lipase, amylase, dextranase, cellulase, pectinase, β-glucosidase, mannanase, xylanase–48 h at 30 °CMuangkeow and Chinajariyawong (2013)Cellulolytic bacteriaCellulase, xylanase, mannanaseFlask4 to 7 d at 30 °CAlshelmani et al. (2013)Cellulolytic and hemicellulolytic bacteriaCellulase, xylanase, mannanaseFlask12 dAlshelmani et al.…”

Section: Solid-state Fermentationmentioning

confidence: 99%

“…Palm kernel cake has been used as an energy source for ruminants and non-ruminants because of its palatability and crude protein content of about 14.5% to 19.24% (Sundu et al., 2006, Ezieshi and Olomu, 2007). The limitation of using palm kernel cake in feeding monogastric animal, especially poultry is due to poor amino acid balance, high indigestible fibre, coarse texture, grittiness, possible deterioration under unhygienic conditions and high concentrations of NSP such as mannan, xylan and cellulose (Alimon, 2004, Sundu et al., 2006, Alshelmani et al., 2013, Alshelmani et al., 2014). The indigestible portions are actually NSP comprising 78% β-mannan, 12% cellulose, 3% arabinoxylans and 3% water-insoluble glucoxylans (Sundu et al., 2006).…”

Section: Effect Of Solid-state Fermentation On the Nutrient Compositimentioning

confidence: 99%

Solid-state fermented plant protein sources in the diets of broiler chickens: A review

et al. 2019

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Protein sources are the second most important component in poultry diets. Due to the fluctuation in price of soybean meal (SBM) and persistent increase in feed prices, nutritionists have been exploring alternative protein sources. Replacement of SBM with alternative protein sources in poultry diets could reduce human-livestock competition for soybean and support the production of more animal protein. However, the use of alternative protein sources is limited to low inclusion due to the presence of anti-nutritional factors (ANF) such as glucosinolates (rapeseed meal), gossypol (cottonseed meal), non-starch polysaccharides (NSP) in lupin flour, high fibre (palm kernel cake), total phenolic contents and phytic acid (canola meal) known to impair animal performance, nutrient digestibility and feed utilization. As a processing technique, solid-state fermentation (SSF) has been researched for a long time in the food industry. An important objective of SSF is the production of enzymes, organic acids and other metabolites of economic importance. In recent times, SSF has been employed to enhance nutrient bioavailability, inhibit gut pathogenic bacteria and reduce ANF in plant protein sources resulting in improved nutrient digestibility, thereby improving performance and gut health of broiler chickens. Unlike pigs, there is still a dearth of information on feeding solid-state fermented feed ingredients to broiler chickens. This review aims to describe the nutritional value of the solid-state fermented products of rapeseed meal, canola meal, cottonseed meal, palm kernel cake and lupin flour on performance and intestinal health of broiler chickens.

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“…T [33][34][35] was obtained from the Bacillus Genetic Stock Center (Columbus, OH). In these assays, P. polymyxa 25A2…”

Section: Cellulolytic Activitymentioning

confidence: 99%

Genome Structure ofBacillus cereustsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis

Zhou

Johnson

et al. 2017

International Journal of Polymer Science

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In previous work, we reported on the isolation and genome sequence analysis of Bacillus cereus strain tsu1 NCBI accession number JPYN00000000. The 36 scaffolds in the assembled tsu1 genome were all aligned with B. cereus B4264 genome with variations. Genes encoding for xylanase and cellulase and the cluster of genes in the poly-3-hydroxybutyrate (PHB) biosynthesis pathway were identified in tsu1 genome. The PHB accumulation in B. cereus tsu1 was initially identified using Sudan Black staining and then confirmed using high-performance liquid chromatography. Physical properties of these PHB extracts, when analyzed with Raman spectra and Fourier transform infrared spectroscopy, were found to be comparable to the standard compound. The five PHB genes in tsu1 (phaA, phaB, phaR, phaC, and phaP) were cloned and expressed with TOPO cloning, and the recombinant proteins were validated using peptide mapping of in-gel trypsin digestion followed by mass spectrometry analysis. The recombinant E. coli BL21 (DE3) (over)expressing phaC was found to accumulate PHB particles. The cellulolytic activity of tsu1 was detected using carboxymethylcellulose (CMC) plate Congo red assay and the shift towards low-molecular size forms of CMC revealed by gel permeation chromatography in CMC liquid culture and the identification of a cellulase in the secreted proteome.

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Characterization of Cellulolytic Bacterial Cultures Grown in Different Substrates

Cited by 17 publications

References 25 publications

Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods

Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods

Solid-state fermented plant protein sources in the diets of broiler chickens: A review

Genome Structure ofBacillus cereustsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis

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