Characterization of cellular elastic modulus using structure based double layer model

Kim, Yeong-Jin; Kim, Mina; Shin, Jennifer Hyunjong; Kim, Jung

doi:10.1007/s11517-010-0730-y

Cited by 18 publications

(16 citation statements)

References 45 publications

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“…where F denotes the deformation gradient, J = det(F ) is the Jacobian of the deformation, and µ 1 , µ 2 and κ are material parameters. For both cytoplasm and nucleus in cancerous cells, material parameters corresponding to the data reported by Kim et al (2011) are chosen and summarized in Table 1. We additionally infer the elastic moduli of the nucleolus from Konno et al (2013) based on a comparison of the relative stiffnesses of the nucleoli and other nuclear domains.…”

Section: Geometry and Materials Parametersmentioning

confidence: 99%

Oncotripsy: Targeting cancer cells selectively via resonant harmonic excitation

Kröger

Ortiz

2016

Journal of the Mechanics and Physics of Solids

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We investigate a method of selectively targeting cancer cells by means of ultrasound harmonic excitation at their resonance frequency, which we refer to as oncotripsy. The geometric model of the cells takes into account the cytoplasm, nucleus and nucleolus, as well as the plasma membrane and nuclear envelope. Material properties are varied within a pathophysiologically-relevant range. A first modal analysis reveals the existence of a spectral gap between the natural frequencies and, most importantly, resonant growth rates of healthy and cancerous cells. The results of the modal analysis are verified by simulating the fullynonlinear transient response of healthy and cancerous cells at resonance. The fully nonlinear analysis confirms that cancerous cells can be selectively taken to lysis by the application of carefully tuned ultrasound harmonic excitation while simultaneously leaving healthy cells intact.

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Section: Geometry and Materials Parametersmentioning

confidence: 99%

Oncotripsy: Targeting cancer cells selectively via resonant harmonic excitation

Kröger

Ortiz

2016

Journal of the Mechanics and Physics of Solids

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“…This can give a reasonable and consistent measurement of the cell’s elastic response to external forces, but does not give specific information about the individual components being measured. Models have been proposed which isolate elastic modulus information from various portions of a force vs. indentation curve to accommodate the multiple-layers encountered by the AFM tip [14,15]. This type of model may be preferred by researchers interested in isolating the individual contributions within multi-layer systems to AFM-acquired force vs. indentation curves.…”

Section: Cellular Topography and Elastic Properties Measured By Afmmentioning

confidence: 99%

Neuron Biomechanics Probed by Atomic Force Microscopy

Spedden

Staii

2013

IJMS

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Mechanical interactions play a key role in many processes associated with neuronal growth and development. Over the last few years there has been significant progress in our understanding of the role played by the substrate stiffness in neuronal growth, of the cell-substrate adhesion forces, of the generation of traction forces during axonal elongation, and of the relationships between the neuron soma elastic properties and its health. The particular capabilities of the Atomic Force Microscope (AFM), such as high spatial resolution, high degree of control over the magnitude and orientation of the applied forces, minimal sample damage, and the ability to image and interact with cells in physiologically relevant conditions make this technique particularly suitable for measuring mechanical properties of living neuronal cells. This article reviews recent advances on using the AFM for studying neuronal biomechanics, provides an overview about the state-of-the-art measurements, and suggests directions for future applications.

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“…Beyond this point, the elastic modulus stabilized, and statistically distinctive values for the normal and cancerous cells were obtained. With a spherical tip, a high modulus region near the plasma membrane existed, and the modulus increased beyond 500 nm as the indentation depth increased, which may have stemmed from either the nucleus, which has a stiffer modulus than the rest of the cytoplasm (Dong et al 1991;Maniotis et al 1997;Caille et al 2002;Kim et al 2011), or the rigid glass substrate. Because the spherical tip was bulkier, the substrate effect was more severe for the spherical tip than the conical tip.…”

Section: Cellular Properties According To the Hertz á Sneddon Modelmentioning

confidence: 99%

Comparative study on the differential mechanical properties of human liver cancer and normal cells

Kim

Hong

Kim

et al. 2013

Animal Cells and Systems

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Although cancerous cells and normal cells are known to have different elasticity values, there have been inconsistent reports in terms of the actual and relative values for these two cell types depending on the experimental conditions. This paper investigated the mechanical characterization of normal hepatocytes (THLE-2) and hepatocellular carcinoma cells (HepG2) using atomic force microscopy indentation experiments and the HertzÁSneddon model, and the results were confirmed by an independent de-adhesion assay. To improve the reliability of the data, we considered the effects of tip geometry and indentation depth on the measured elasticity of the cells. This study demonstrated that THLE-2 cells had a higher elastic modulus compared with the HepG2 cells and that this difference was more significant when a conical tip was used. The inhibitor study indicated that this difference in the mechanical properties of THLE-2 and HepG2 cells was mainly attributed to differential arrangements in the cytoskeletal networks of actin filaments. An independent de-adhesion assay also confirmed that THLE-2 cells had a higher elastic modulus compared with the HepG2 cells, which resulted in a shorter time constant for cellular contractility.

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Characterization of cellular elastic modulus using structure based double layer model

Cited by 18 publications

References 45 publications

Oncotripsy: Targeting cancer cells selectively via resonant harmonic excitation

Oncotripsy: Targeting cancer cells selectively via resonant harmonic excitation

Neuron Biomechanics Probed by Atomic Force Microscopy

Comparative study on the differential mechanical properties of human liver cancer and normal cells

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