Characterization of cDNAs Encoding Small and Large Subunits of ADP-Glucose Pyrophosphorylases from Watermelon (Citrullus vulgaris S.).

Kim, In-Jung; Kahng, Hyung-Yeel; Chung, Won Sang

doi:10.1271/bbb.62.550

Cited by 13 publications

(19 citation statements)

References 23 publications

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“…AF032472) (Kim et al , 1998): P1, 5′-AAACTTCCTCTAACTTTCTCACC-3′; and P2, 5′-TCCAGACCACCCCAATTACCCTT-3′. Uneven PCRs were conducted according to the methods of Chen et al (1997).…”

Section: Methodsmentioning

confidence: 99%

Two negative cis-regulatory regions involved in fruit-specific promoter activity from watermelon (Citrullus vulgaris S.)

Yin¹,

Wu²,

Zhang³

et al. 2008

Journal of Experimental Botany

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A 1.8 kb 5′-flanking region of the large subunit of ADP-glucose pyrophosphorylase, isolated from watermelon (Citrullus vulgaris S.), has fruit-specific promoter activity in transgenic tomato plants. Two negative regulatory regions, from –986 to –959 and from –472 to –424, were identified in this promoter region by fine deletion analyses. Removal of both regions led to constitutive expression in epidermal cells. Gain-of-function experiments showed that these two regions were sufficient to inhibit RFP (red fluorescent protein) expression in transformed epidermal cells when fused to the cauliflower mosaic virus (CaMV) 35S minimal promoter. Gel mobility shift experiments demonstrated the presence of leaf nuclear factors that interact with these two elements. A TCCAAAA motif was identified in these two regions, as well as one in the reverse orientation, which was confirmed to be a novel specific cis-element. A quantitative β-glucuronidase (GUS) activity assay of stable transgenic tomato plants showed that the activities of chimeric promoters harbouring only one of the two cis-elements, or both, were ∼10-fold higher in fruits than in leaves. These data confirm that the TCCAAAA motif functions as a fruit-specific element by inhibiting gene expression in leaves.

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“…AF032472) (Kim et al , 1998): P1, 5′-AAACTTCCTCTAACTTTCTCACC-3′; and P2, 5′-TCCAGACCACCCCAATTACCCTT-3′. Uneven PCRs were conducted according to the methods of Chen et al (1997).…”

Section: Methodsmentioning

confidence: 99%

Two negative cis-regulatory regions involved in fruit-specific promoter activity from watermelon (Citrullus vulgaris S.)

Yin¹,

Wu²,

Zhang³

et al. 2008

Journal of Experimental Botany

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“…The same authors suggested this disulfide is formed through a non‐enzymatic oxidation of 4‐mercaptobutyl‐GLS during the extraction and purification steps 9–12. A second compound, observed as a prominent peak in extracts of rocket salad, was isolated and identified to be 4‐(β‐D‐glucopyranosyldisulfanyl)butyl‐GLS (4‐GDB‐GLS) by Kim et al 13, 14. The simultaneous occurrence of both these compounds having two glucose moieties and an S‐S bond as intact GLSs has not been described before.…”

mentioning

confidence: 97%

Naturally occurring glucosinolates in plant extracts of rocket salad (Eruca sativa L.) identified by liquid chromatography coupled with negative ion electrospray ionization and quadrupole ion‐trap mass spectrometry

Cataldi

Rubino

Lelario

et al. 2007

Rapid Comm Mass Spectrometry

101

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A method for the comprehensive profiling of intact glucosinolates (GLSs), major and minor, occurring in leaves and seeds of rocket salad (Eruca sativa L.) is presented using optimized reversed-phase liquid chromatography (RP-LC) with electrospray ionization (ESI) ion trap mass spectrometry (ITMS). ESI-ITMS in the negative mode was confirmed to be very suitable to analyze these compounds in crude extracts. After extraction from the plant material with methanol/water (70:30 v/v) at 70 degrees C, the analytes of interest were separated on a C18 column using an eluent acidified with formic acid (0.1%) and modified with acetonitrile. All the GLSs found in leaves of rocket salad gave good signals corresponding to the deprotonated precursor ion, [M-H]-. Although the mass spectra also exhibited an analytically important non-covalent adduct ion at [2M-H]-, the structures of glucosinolates were confirmed by extensive sequential MS analysis, thereby substantially improving the identification of unknown compounds. The results obtained not only revealed in leaves of E. sativa at least twelve species of GLSs including seven aliphatic compounds (glucoraphanin with [M-H]- at m/z ratio of 436, glucoerucin at m/z 420, 4-mercaptobutyl-GLS at m/z 406, progoitrin/epiprogoitrin at m/z 388, sinigrin at m/z 358, 4-methylpentyl- and n-hexyl-GLS at m/z 402) and three indole glucosinolates (i.e., three N-heterocyclic compounds: 4-hydroxyglucobrassicin and 5-hydroxyglucobrassicin at m/z 463, and 4-methoxy-glucobrassicin at m/z 477), but also two structurally related compounds containing one intermolecular disulfide linkage (4-(beta-D-glucopyranosyldisulfanyl)butyl-GLS at m/z 600 and a dimeric 4-mercaptobutyl-GLS at m/z 811). This latter symmetric disulfide was previously considered as an artefact formed during extraction of GLSs from vegetative tissues. Glucosinolates were detected in the leaves with a wide range of contents (10-200 micromol/g) and a great variation in the composition. Only three GLSs were identified in seeds of rocket salad, namely glucoraphanin, glucoerucin and 4-methoxyglucobrassicin. As expected, the most abundant GLS in seeds is glucoerucin. The feasibility of the strategy was also demonstrated using a rapeseed extract of certified reference material (BCR367R). The results indicated the usefulness of this method for a rapid, sensitive and comprehensive profiling of the GLS family naturally occurring in extracts of crude plant matter.

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“…Geralmente, ocorrem decréscimos nos teores de açúcares, durante o armazenamento, principalmente de frutos que não acumulam reservas de amido, haja vista que são utilizados como substrato respiratório para manutenção do metabolismo celular (Kader, 2002;Taiz & Zeiger, 2004). Não obstante, em melancia, há evidencias de acúmulo de amido no final do desenvolvimento do fruto (Kim et al, 1998) o que pode explicar o comportamento de acréscimo (nos frutos cultivados com 80 e 289 kg/ ha P 2 O 5 aplicadas em fundação e cobertura) ou manutenção dos teores de AS nos frutos aos 35 dias de armazenamento.…”

Section: Resultsunclassified

Efeito de diferentes doses, formas de aplicação e fontes de P na conservação de melancia sem sementes

et al. 2016

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RESUMOO trabalho teve como objetivo avaliar o efeito de doses, fontes e formas de aplicação de P na conservação de melancia sem semente. Para isto, foi realizado o plantio da melancia cv. 'Style'. Os tratamentos consistiram de duas doses de P (80 e 289 kg/ha P 2 O 5 ) em duas formas de aplicação (fundação e cobertura), utilizando o superfosfato triplo, na fundação, e o fosfato monoamônico (MAP) em cobertura. Ainda, foi acrescentado um tratamento adicional com a maior dose (289 kg/ha P 2 O 5 ) parcelada com MAP em fundação e cobertura, totalizando cinco tratamentos. No laboratório, foi instalado um experimento inteiramente casualizado em esquema fatorial 5x5, sendo as cinco adubações fosfatadas e cinco períodos de armazenamento dos frutos (0, 14, 21, 28 e 35 dias após a colheita), com quatro repetições. Uma amostra de oito frutos foi avaliada no tempo zero e os demais armazenados em temperatura de 9±2°C e 85±2% UR, onde permaneceram por 7, 14, 21 e 28 dias e mais sete dias à temperatura de 23±2°C e 60±2% UR. Foram avaliados nos frutos a massa, comprimento, diâmetro, espessura de polpa, formato, perda de massa (PM), firmeza da polpa (FP), sólidos solúveis (SS), acidez titulável (AT), açúcares solúveis (AS), relação SS/AT e licopeno. A adubação (doses, forma de aplicação e fontes de P) não afetou a massa, comprimento, diâmetro, espessura de polpa, formato do fruto, perda de massa, firmeza de polpa e teor de licopeno dos frutos. Todavia, houve interação entre os fatores adubação e armazenamento, para AS e relação SS/AT. Houve acréscimos na PM e decréscimos nos teores de SS, AS, AT e licopeno dos frutos durante o armazenamento. Porém, a adubação fosfatada, na menor dose (80 kg/ha) aplicada via fundação, resultou em melhor resultado na conservação pós-colheita da melancia sem semente, mantendo-se maior teor de AS e relação SS/AT da polpa até 35 dias de armazenamento. Palavras-chave:Citrullus lanatus, adubação, pós-colheita. ABSTRACT Effect of different doses, application forms and sources of P in the conservation of seedless watermelonThe effect of doses, sources and application forms of P was evaluated for the conservation of seedless watermelon. With this purpose we planted the watermelon cv. Style. Treatments consisted of two doses of P (80 and 289 kg/ha P 2 O 5 ) in two application forms (foundation and cover), using triple superphosphate on the foundation, and monoammonium phosphate (MAP) on coverage. Moreover, an additional treatment was evaluated, using the highest dose (289 kg/ ha P 2 O 5 ) splitted with MAP in the foundation and cover, totalling five treatments. In the lab, a complete randomized factorial research was installed, in a factorial 5x5, with five phosphate fertilizations and five fruit storage periods (0, 14, 21, 28 and 35 days after harvest), with four replications. A sample of eight fruits was evaluated at time zero and the other fruits were stored at a temperature of 9±2°C and 85±2% RH, remaining for 7, 14, 21 and 28 days and, additional seven days at a temperature of 23±2°C and 60±2% RH. We ev...

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Characterization of cDNAs Encoding Small and Large Subunits of ADP-Glucose Pyrophosphorylases from Watermelon (Citrullus vulgaris S.).

Cited by 13 publications

References 23 publications

Two negative cis-regulatory regions involved in fruit-specific promoter activity from watermelon (Citrullus vulgaris S.)

Two negative cis-regulatory regions involved in fruit-specific promoter activity from watermelon (Citrullus vulgaris S.)

Naturally occurring glucosinolates in plant extracts of rocket salad (Eruca sativa L.) identified by liquid chromatography coupled with negative ion electrospray ionization and quadrupole ion‐trap mass spectrometry

Efeito de diferentes doses, formas de aplicação e fontes de P na conservação de melancia sem sementes

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