Characterization of Cationic Modified Short Linear Glucan and Fabrication of Complex Nanoparticles with Low and High Methoxy Pectin

Abstract

In this study, we chemically modified the short linear glucan (SLG) using the 3-chloro-2-hydroxypropyl trimethylammonium chloride to introduce a positive surface charge via cationization (CSLG). We then prepared CSLG-based binary nanocomplex particles through electrostatic interactions with low and high methoxyl pectin. The two new types of binary nanocomplex were comprehensively characterized. It was found that the nanocomplex particles showed a spherical shape with the particle size of < 700 nm, smooth surface, homogeneous distribution, and negative surface charge. Fourier transform infrared spectroscopy (FTIR) revealed that the driving forces to form nanocomplex were primarily electrostatic interactions and hydrogen bonding. In addition, increasing the CSLG concentration in the nanocomplex significantly enhanced both thermal stability and digestive stability. By comparing the two complex nanoparticles, the HMP-CSLG has a larger particle size and better stability under the GI condition due to the high content of the methoxy group. Additionally, the HMP-CSLG nanoparticle has a higher encapsulation efficiency and slower release rate under simulated gastrointestinal fluid for tangeretin compared with the LMP-CSLG. These results provide new insights into designing the CSLG-based nanocomplex as a potential oral delivery system for nutraceuticals or active ingredients.

Elaboration of Cationic Soluble Soybean Polysaccharides-Epigallocatechin Gallate Nanoparticles with Sustained Antioxidant and Antimicrobial Activities

“…Liu et al reported that the ζ-potentials of cationic debranched starch increased from +15.7 to +35.05 mV with the quaternization degree increasing from 0.29 to 1.14 . Wu et al observed that the ζ-potentials of cationic short linear glucan increased from +22.18 to +34.52 mV with the quaternization degree increasing from 0.36 to 0.94 …”

“…23 Wu et al observed that the ζ-potentials of cationic short linear glucan increased from +22.18 to +34.52 mV with the quaternization degree increasing from 0.36 to 0.94. 25 The combination of cationic polysaccharides with anionic bioactives/drugs may result in inter-polyelectrolyte complexes with micro-and nanoscale structures, further expanding the applications of the latter. This is largely based on the protection and slow-release effect of the former on the latter.…”

“…Cationic polysaccharides are commonly adopted in various fields, including paper-making, water treatment, food, biopharmaceuticals, cosmetics, and the chemical industry. 22 Cationization of polysaccharides including debranched starch, 23 cellulose and its derivatives, 24 and glucan 25 has been investigated. Cationized polysaccharides may be acquired through different reagent reactions.…”