Characterization of catechol 2,3-dioxygenase from Planococcus sp. strain S5 induced by high phenol concentration.

Hupert-Kocurek, Katarzyna; Guzik, Urszula; Wojcieszyńska, Danuta

doi:10.18388/abp.2012_2119

Cited by 51 publications

(32 citation statements)

References 70 publications

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“…In contrast, C23O from Planococcus sp. strain S5 showed higher activity towards 4chlorocatechol than catechol and methylcatechols (Hupert-Kocurek et al 2012). As 4,5-dichlorocatechol is a product of 2,4-dichlorophenol degradation in KB2, activity of C23O for this substrate was also observed.…”

Section: Substrate Specifi City Of C23o From Kb2mentioning

confidence: 89%

Factors affecting activity of catechol 2,3-dioxygenase from 2-chlorophenol-degradingStenotrophomonas maltophiliastrain KB2

Wojcieszyńska

Hupert-Kocurek

2013

Biocatalysis and Biotransformation

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The effect of phenol on 2-chloro-and 2,4-dichlorophenol degradation by Stenotrophomonas maltophilia KB2 has been studied. During this study, we observed induction of catechol 2,3-dioxygenase (C23O). Since, in the environment, compounds which inhibit C23O activity are frequently present together with the main dioxygenase substrates, the main aim of this work was to determine the infl uence of various inhibitors and activators on the enzyme activity. Hydrogen peroxide of 60 μ M concentration caused total inhibition of the enzyme. Addition of ascorbic acid suppressed the inhibitory effect of hydrogen peroxide. In its presence, 60 μ M hydrogen peroxide caused only 40% inhibition of C23O activity. A positive effect in preventing C23O activity was observed also in the presence of chelators (8-hydroxyquinoline, EDTA, and phenanthroline). Most metal ions and aliphatic and aromatic hydroxylated derivatives caused a 20 -40% decrease in enzyme activity. The results obtained indicate that C23O from Stenotrophomonas maltophilia strain KB2 holds great potential for bioremediation.

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Section: Substrate Specifi City Of C23o From Kb2mentioning

confidence: 89%

Factors affecting activity of catechol 2,3-dioxygenase from 2-chlorophenol-degradingStenotrophomonas maltophiliastrain KB2

Wojcieszyńska

Hupert-Kocurek

2013

Biocatalysis and Biotransformation

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“…The degradative potential of six strains: Planococcus sp. S5, Stenotrophomonas maltophilia KB2, Pseudomonas putida N6, Pseudomonas sp., Pseudomonas putida mt-2, and Pseudomonas fluorescens towards a variety of organic compounds has previously been reported in the literature [36][37][38], whereas four strains: Pseudomonas putida OR45a, Pseudomonas putida KB3, Glutamicibacter soli OR45b, and Rhodococcus erythropolis KB4 have only recently been isolated from the environment and are presented here for the first time.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Selecting Bacteria Candidates for the Bioaugmentation of Activated Sludge to Improve the Aerobic Treatment of Landfill Leachate

Michalska

Piński

Żur

et al. 2020

Water

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In this study, a multifaceted approach for selecting the suitable candidates for bioaugmentation of activated sludge (AS) that supports leachate treatment was used. To determine the exploitation of 10 bacterial strains isolated from the various matrices for inoculating the AS contaminated with the Kalina pond leachate (KPL), their degradative potential was analyzed along with their aptitude to synthesize compounds improving remediation of pollutants in wastewater and ability to incorporate into the AS flocs. Based on their capability to degrade aromatic compounds (primarily catechol, phenol, and cresols) at a concentration of 1 mg/mL and survive in 12.5% of the KPL, Pseudomonas putida OR45a and P. putida KB3 can be considered to be the best candidates for bioaugmentation of the AS among all of the bacteria tested. Genomic analyses of these two strains revealed the presence of the genes encoding enzymes related to the metabolism of aromatic compounds. Additionally, both microorganisms exhibited a high hydrophobic propensity (above 50%) and an ability to produce biosurfactants as well as high resistance to ammonium (above 600 µg/mL) and heavy metals (especially chromium). These properties enable the exploitation of both bacterial strains in the bioremediation of the AS contaminated with the KPL.

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“…The majority of studies on the detection of catabolic genes in contaminated environments have focused on gram-negative bacteria, such as Pseudomonas , Burkholderia , Acinetobacter and Sphingomonas 26 . These strains usually carry one or two dioxygenase genes, 27 but catechol 2,3-dioxygenases constitute a group of enzymes that are considered crucial for the degradation of a wide range of aromatic compounds in contaminated habitats, and they are present in most aromatic compound-degrading strains 28, 29. Because these dioxygenase genes are commonly distributed between plasmids of different sizes and are found on the chromosome, 30 a suggestion has been made that these genes may spread to divergent bacteria by horizontal transfer 31 .…”

Section: Discussionmentioning

confidence: 99%

Phenol degradation and genotypic analysis of dioxygenase genes in bacteria isolated from sediments

Tian

Zhou

et al. 2017

Brazilian Journal of Microbiology

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The aerobic degradation of aromatic compounds by bacteria is performed by dioxygenases. To show some characteristic patterns of the dioxygenase genotype and its degradation specificities, twenty-nine gram-negative bacterial cultures were obtained from sediment contaminated with phenolic compounds in Wuhan, China. The isolates were phylogenetically diverse and belonged to 10 genera. All 29 gram-negative bacteria were able to utilize phenol, m-dihydroxybenzene and 2-hydroxybenzoic acid as the sole carbon sources, and members of the three primary genera Pseudomonas, Acinetobacter and Alcaligenes were able to grow in the presence of multiple monoaromatic compounds. PCR and DNA sequence analysis were used to detect dioxygenase genes coding for catechol 1,2-dioxygenase, catechol 2,3-dioxygenase and protocatechuate 3,4-dioxygenase. The results showed that there are 4 genotypes; most strains are either PNP (catechol 1,2-dioxygenase gene is positive, catechol 2,3-dioxygenase gene is negative, protocatechuate 3,4-dioxygenase gene is positive) or PNN (catechol 1,2-dioxygenase gene is positive, catechol 2,3-dioxygenase gene is negative, protocatechuate 3,4-dioxygenase gene is negative). The strains with two dioxygenase genes can usually grow on many more aromatic compounds than strains with one dioxygenase gene. Degradation experiments using a mixed culture representing four bacterial genotypes resulted in the rapid degradation of phenol. Determinations of substrate utilization and phenol degradation revealed their affiliations through dioxygenase genotype data.

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Characterization of catechol 2,3-dioxygenase from Planococcus sp. strain S5 induced by high phenol concentration.

Cited by 51 publications

References 70 publications

Factors affecting activity of catechol 2,3-dioxygenase from 2-chlorophenol-degradingStenotrophomonas maltophiliastrain KB2

Factors affecting activity of catechol 2,3-dioxygenase from 2-chlorophenol-degradingStenotrophomonas maltophiliastrain KB2

Selecting Bacteria Candidates for the Bioaugmentation of Activated Sludge to Improve the Aerobic Treatment of Landfill Leachate

Phenol degradation and genotypic analysis of dioxygenase genes in bacteria isolated from sediments

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