Characterization of caseins from Mongolian yak, khainak, and bactrian camel

Ochirkhuyag, Baldorj; Chobert, Jean‐Marc; Dalgalarrondo, Michèle; Choiset, Yvan; Haertlé, Thomas

doi:10.1051/lait:1997543

Cited by 38 publications

(26 citation statements)

References 17 publications

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“…L -1 and 40 g·L -1 acrylamide, respectively. The acrylamide gel (100 g·L -1 in 4 mol·L -1 urea, Tris-HCl, pH 8.8, buffer solution) for urea-PAGE was prepared as described previously [24]. The silver staining of the gel was carried out according to Nesterenko et al [23], and staining with Schiff's reagent was according to Grooves et al [12].…”

Section: Polyacrylamide Gel Electrophoresis (Page)mentioning

confidence: 99%

“…The amino acids were derivatized with phenylisothiocyanate (PITC) according to the method of Bidlingmeyer et al [4] and separated by RP-HPLC on a Pico-Tag C 18 column (3.9 × 150 mm). The column was equilibrated in solvent A (94% 0.14 mol·L -1 CH 3 COONa, 0.5 mL·L -1 triethylamine, pH 6.4/6% acetonitrile) and the elution was performed by using a gradient from solvent A to solvent B (40% H 2 O/60% acetonitrile) as previously described [24]. Both the column and solvents were maintained at 38 °C.…”

Section: Amino Acid Composition Analysismentioning

confidence: 99%

“…* Data on cow, goat, mouse and pig caseins are fiom the Swissprot database; ¦ according to Ochirkhuyag et al [24]; § according to Kappeler et al [17].…”

Section: Thermally Precipitated Casein Subfractionsmentioning

confidence: 99%

“…* Les données concernant les caséines de vache, chèvre, souris et porc sont issues de la base de données Swissprot ; ¦ selon Ochirkhuyag et al [24] ; § selon Kappeler et al [17].…”

Section: Thermally Precipitated Casein Subfractionsunclassified

“…Surprisingly, the situation is quite the opposite in the case of equine caseins, about which even less is known than caseins from different Camelidae [2,9,17,20,22,24].…”

Section: Introductionmentioning

confidence: 96%

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Characterization of mare caseins. Identification of $\alpha_{{\bf S1}}$- and $\alpha_{{\bf S2}}$- caseins

Ochirkhuyag

Chobert²,

Dalgalarrondo³

et al. 2000

Lait

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-Whole mare (Mongolian and French breeds) casein was obtained from skim milk by isoelectric precipitation (pH 4.6) at 22 °C. In another series of experiments, equine caseins were fractionated after isoelectric precipitation at 4 °C, according to their sensitivity to temperature, on one hand, the pH of the resulting pellet was adjusted to 6.5 before centrifugation (45 000 g for 30 min) at 4 °C; on the resulting casein fraction which precipitated was named the cold precipitated (CP) fraction. On the other hand, the supernatant obtained from the centrifugation of skim milk at pH 4.6 and 4 °C was warmed to 30 °C before being centrifuged at 30 °C, 45 000 g, for 30 min. The resulting casein fraction which precipitated was named the thermally precipitated (TP) fraction. Equine caseins were then purified by high resolution gel chromatography on an anion-exchange column followed by reversed phase-high performance liquid chromatography. The casein fractions were analyzed by urea-and SDS-polyacrylamide gel electrophoresis, their amino acid compositions were determined and their first 15 N-terminal amino acids were sequenced. This analysis showed the presence of α slike caseins isolated from the CP fraction. α s1 -Like-casein showed 4 major double bands by electrofocusing with a pI range of 4.3-4.8. In the same conditions, α s2 -like casein showed 2 major bands with a pI range of 4.3-5.1. The TP fraction revealed the existence in equine milk of 6 subfractions of β-like caseins, occurring in the following ratios: 1:5:18:20:15:10, differing at least in their degree of phosphorylation (as shown also by the action of acid phosphatase). Since no evidence of the presence of κ-casein was found in equine milk, it is proposed that part of its functions in the milk of the Equidae could be assumed by the population of less phosphorylated β-caseins.

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Section: Polyacrylamide Gel Electrophoresis (Page)mentioning

confidence: 99%

Section: Amino Acid Composition Analysismentioning

confidence: 99%

“…* Data on cow, goat, mouse and pig caseins are fiom the Swissprot database; ¦ according to Ochirkhuyag et al [24]; § according to Kappeler et al [17].…”

Section: Thermally Precipitated Casein Subfractionsmentioning

confidence: 99%

“…* Les données concernant les caséines de vache, chèvre, souris et porc sont issues de la base de données Swissprot ; ¦ selon Ochirkhuyag et al [24] ; § selon Kappeler et al [17].…”

Section: Thermally Precipitated Casein Subfractionsunclassified

“…Surprisingly, the situation is quite the opposite in the case of equine caseins, about which even less is known than caseins from different Camelidae [2,9,17,20,22,24].…”

Section: Introductionmentioning

confidence: 96%

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