Characterization of casein peptones by HPLC profiles and microbiological growth parameters

Reissbrodt, Rolf; Beer, W.; Claus, H.; Müller, Rita

doi:10.1002/abio.370150212

Cited by 18 publications

(6 citation statements)

References 4 publications

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“…The results were in agreement with results reported by others about alcalase efficiency in fish by-product hydrolysis (Ovissipour et al 2009). However, Reissbrodt et al (1995) mentioned that to evaluate peptone efficiency in growth media, physical and chemical data alone are not sufficient to understand the overall effect on microbial growth. Regarding the fact that peptones in microbial media provides the main sources of nitrogen and carbon, a successful peptone source is better evaluated by the growth of the organism of interest (Vieira et al 2005).…”

Section: Chemical Compositionmentioning

confidence: 99%

Fish peptone development using enzymatic hydrolysis of silver carp by‐products as a nitrogen source in Staphylococcus aureus media

Fallah

Soleymani

Javadian

2015

Food Science & Nutrition

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Fish peptone was produced using enzymatic hydrolysis of silver carp filleting by-products by alcalase and trypsin. Also, the efficiency of the hydrolysates as a nitrogen source in Staphylococcus aureus medium was compared with commercial TSB. The results indicated that the protein hydrolysate from alcalase and trypsin had high protein content (92.92%, 91.53 respectively), and degree of hydrolysis (4.94%, 4.6% respectively).The results showed that silver carp filleting waste can be an efficient source for fish peptone production as a nitrogen source for S. aureus medium. However, the type of the used proteolytic enzyme considerably affected the performance of the resulting peptone despite the same DH. Fish peptone produced by alcalese performed significantly (P < 0.05) better than commercial TSB as a media for the bacteria while the performance of the trypsin peptone was not as good as the commercial medium.

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Section: Chemical Compositionmentioning

confidence: 99%

Fish peptone development using enzymatic hydrolysis of silver carp by‐products as a nitrogen source in Staphylococcus aureus media

Fallah

Soleymani

Javadian

2015

Food Science & Nutrition

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“…Organic nitrogen substrates, such as peptones, are widely used in many biological and biotechnological applications, such as microbial biomass production (Singh et al 1995), and metabolite biosynthesis including enzymes (Haltrich et al 1994;Rapp 1995). At present, peptones are obtained from casein, soy protein, gelatin and meat (Reed Hamer and West 1994;Reissbrodt et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Preparation and testing of Sardinella protein hydrolysates as nitrogen source for extracellular lipase production by Rhizopus oryzae

Ghorbel¹,

Souissi²,

Triki-Ellouz³

et al. 2005

World J Microbiol Biotechnol

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Various fish protein hydrolysates (FPH) from sardinella (Sardinella aurita) were used as nitrogen sources for the production of extracellular lipase by the filamentous fungus Rhizopus oryzae. The best results were obtained with defatted meat-fish protein hydrolysates (DMFPH), indicating the presence in the lipid fraction of some constituents which may repress lipase synthesis. Furthermore, it was found that the extensive hydrolysis of fish proteins resulted in a higher lipase production. The use of 40 g DMFPH l )1 for the growth of Rhizopus oryzae in medium R1 resulted in a lipase production of 394 U ml )1 , higher than the yield obtained with standard soy peptone as nitrogen source (373 U ml )1 ). The most appropriate medium for the growth and the production of lipase is composed only of 24 g DMFPH l )1 and 10 g glucose l )1 , indicating that the strain can obtain its nitrogen and salts requirements directly from fish substrate.

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“…Peptone is generally used in culture media because of its rich amino acid and low-molecular weight peptide content. Peptones are generally obtained from casein (Reed and West 1994), gelatin and soya meal (Reissbrodt et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of extracellular protease of mutant Aspergillus niger AB100 grown on fish scale

Basu

Banik

Das

2007

World J Microbiol Biotechnol

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Fish scale, the chief waste material of fish processing industries was processed and tested for production of extracellular protease by mutant Aspergillus niger AB 100 . Protease production by A. niger AB 100 was greatly enhanced in presence of processed fish scale powder. Where as among the three complex nutrients tested, soya bean meal shows maximum stimulatory effect over protease production (2,776 lmol/ml/min) when used in combination with glucose (5% w/v) and urea (2.5% w/v). The protease was optimally active at pH 7.0, retaining more than 60% of its activity in the pH range of 5-9. The enzyme was found to be most active at 50°C and stable at 30°C for 1 h. Purification of enzyme by CM-Cellulose and SDS-PAGE resulted in about 26-fold increase in the specific activity of the enzyme with a molecular weight of 30.9 kDa. HPLC study shows the purity of the enzyme as 75.92%. By the activating effect of divalent cations (Fe 2+ , Zn 2+ , Mn 2+ , Ca 2+ and Mg 2+ ) and inhibiting effect of chelating agent (EDTA) and Hg 2+ , the enzyme was found to be a metalloprotease.

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Characterization of casein peptones by HPLC profiles and microbiological growth parameters

Cited by 18 publications

References 4 publications

Fish peptone development using enzymatic hydrolysis of silver carp by‐products as a nitrogen source in Staphylococcus aureus media

Fish peptone development using enzymatic hydrolysis of silver carp by‐products as a nitrogen source in Staphylococcus aureus media

Preparation and testing of Sardinella protein hydrolysates as nitrogen source for extracellular lipase production by Rhizopus oryzae

Production and characterization of extracellular protease of mutant Aspergillus niger AB100 grown on fish scale

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