Characterization of carp seminal plasma Wap65-2 and its participation in the testicular immune response and temperature acclimation

Dietrich, Mariola A.; Adamek, Mikołaj; Jung‐Schroers, Verena; Rakus, Krzysztof; Chadzińska, Magdalena; Hejmej, Anna; Hliwa, Piotr; Bilińska, Barbara; Karol, Halina; Ciereszko, Andrzej

doi:10.1186/s13567-020-00858-x

Cited by 7 publications

(3 citation statements)

References 53 publications

(84 reference statements)

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“…The data are expressed in arbitrary units according to the manufacturer’s instructions (Bio-Rad) and Posch et al [ 69 ]. To confirm the specificity of the antibodies used, protein bands corresponding to the detected bands were manually removed from the gels and prepared for digestion and protein identification using MALDI-TOF/TOF [ 70 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

Kodzik,

Ciereszko,

Szczepkowska

et al. 2024

BMC Genomics

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Background Sturgeon species are living fossils that exhibit unique reproductive characteristics, and elucidation of the molecular processes governing the formation and quality of sturgeon eggs is crucial. However, comprehensive data on the protein composition of sturgeon ovarian fluid (OF) and eggs and their functional significance are lacking. To address this knowledge gap, the aim of the present study was to conduct a comprehensive comparative proteomic analysis of Siberian sturgeon OF and eggs using liquid chromatography–mass spectrometry (LC–MS/MS). Results A total of 617 proteins were identified in OF, and 565 proteins were identified in eggs. A total of 772 proteins showed differential abundance. Among the differentially abundant proteins, 365 were more abundant in OFs, while 407 were more abundant in eggs. We identified 339 proteins unique to OFs and 287 proteins specific to eggs, and further investigated the top 10 most abundant proteins in each. The functional annotation of the OF proteins highlighted their predominant association with immune system processes, including the complement and coagulation cascade, neutrophil and leukocyte-mediated immunity, cholesterol metabolism, and regulation of the actin cytoskeleton. Analysis of egg proteins revealed enrichment in metabolic pathways, such as oxidative phosphorylation and fatty acid metabolism, and protein ubiquitination and translation. OF-specific proteins included extracellular matrix and secretory vesicles, and eggs were enriched in proteins localized to mitochondria and ribosome components. Conclusions This study presents the first comprehensive characterization of the protein composition of sturgeon OF and eggs and elucidates their distinct functional roles. These findings advance our understanding of sturgeon reproduction, OF-egg signaling and the origin of OF proteins. The mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier PXD044168 to ensure accessibility for further research.

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Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

Kodzik,

Ciereszko,

Szczepkowska

et al. 2024

BMC Genomics

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“…In case of both viral infection liver tissues were used. Bacterial infection with Aeromonas salmonicida was performed by intraperitoneal injection of R3xR8 cross as described here ( 26 ), liver, spleen, kidney and testis were used. Infections were performed at specific temperatures optimal for each pathogen: 18°C for CEV, 23°C for KHV and 25°C for A. salmonicida .…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was isolated from the tissues using the TRI reagent (Sigma) and transcribed to cDNA using 100 U Maxima Reverse Transcriptase (Thermo Fisher Scientific) as described earlier ( 7 , 24 – 26 ). cDNA samples were diluted 1:40 with nuclease-free water (Thermo Fisher Scientific) before qPCR analysis.…”

Section: Methodsmentioning

confidence: 99%

Secreted novel AID/APOBEC-like deaminase 1 (SNAD1) – a new important player in fish immunology

Majewska,

Dietrich,

Budzko

et al. 2024

Front. Immunol.

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The AID/APOBECs are a group of zinc-dependent cytidine deaminases that catalyse the deamination of bases in nucleic acids, resulting in a cytidine to uridine transition. Secreted novel AID/APOBEC-like deaminases (SNADs), characterized by the presence of a signal peptide are unique among all of intracellular classical AID/APOBECs, which are the central part of antibody diversity and antiviral defense. To date, there is no available knowledge on SNADs including protein characterization, biochemical characteristics and catalytic activity. We used various in silico approaches to define the phylogeny of SNADs, their common structural features, and their potential structural variations in fish species. Our analysis provides strong evidence of the universal presence of SNAD1 proteins/transcripts in fish, in which expression commences after hatching and is highest in anatomical organs linked to the immune system. Moreover, we searched published fish data and identified previously, “uncharacterized proteins” and transcripts as SNAD1 sequences. Our review into immunological research suggests SNAD1 role in immune response to infection or immunization, and interactions with the intestinal microbiota. We also noted SNAD1 association with temperature acclimation, environmental pollution and sex-based expression differences, with females showing higher level. To validate in silico predictions we performed expression studies of several SNAD1 gene variants in carp, which revealed distinct patterns of responses under different conditions. Dual sensitivity to environmental and pathogenic stress highlights its importance in the fish and potentially enhancing thermotolerance and immune defense. Revealing the biological roles of SNADs represents an exciting new area of research related to the role of DNA and/or RNA editing in fish biology.

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Temperature alters the oxidative and metabolic biomarkers and expression of environmental stress-related genes in chocolate mahseer (Neolissochilus hexagonolepis)

Dash

Siva

Tandel

et al. 2023

Environ Sci Pollut Res

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Characterization of carp seminal plasma Wap65-2 and its participation in the testicular immune response and temperature acclimation

Cited by 7 publications

References 53 publications

Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

Comparative proteomic analysis of the ovarian fluid and eggs of Siberian sturgeon

Secreted novel AID/APOBEC-like deaminase 1 (SNAD1) – a new important player in fish immunology

Temperature alters the oxidative and metabolic biomarkers and expression of environmental stress-related genes in chocolate mahseer (Neolissochilus hexagonolepis)

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