Characterization of carp seminal plasma proteome in relation to blood plasma

Dietrich, Mariola A.; Arnold, Georg J.; Nynca, Joanna; Fröhlich, Thomas; Otte, Kerstin; Ciereszko, Andrzej

doi:10.1016/j.jprot.2014.01.005

Cited by 57 publications

(40 citation statements)

References 46 publications

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“…Specific isoforms of apolipoproteins were also detected: Apolipoproteins A‐I, A‐II, B and C were previously identified in fish blood (Loir et al, ), while apolipoproteins A and C were reported in fish seminal plasma (Babin and Vernier, ; Ciereszko et al, ); this is the first study to identify multiple isoforms of apolipoprotein E in carp seminal plasma. This new finding indicates that different testis‐specific isoforms are expressed and/or the presence of potential post‐translational modifications (Dietrich et al, ). The variety of distinct apolipoprotein isoforms in carp seminal plasma are likely linked to sperm energy resources and the maintenance of spermatozoon membrane lipid composition.…”

Section: Discussionmentioning

confidence: 89%

Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Shaliutina‐Kolešová

Kotas

Štěrba

et al. 2016

Molecular Reproduction Devel

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The purpose of the study was to analyze seminal plasma composition, sperm production, and sperm motility over the course of the spawning season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss). The highest mean percent of motile sperm (carp, 98.3 ± 1.6%; rainbow trout, 92.8 ± 5.6%) and highest spermatozoon velocity (carp, 286.3 ± 7.8 μm sec ; rainbow trout, 245.3 ± 8.3 μm sec ) were observed in the middle phase of the spawning period, at 5 sec post-activation. Sperm volume and concentration increased in the early spawning period, then decreased significantly toward the end of the season in both species. Seminal plasma osmolality was 262-270 mOsmol kg from carp and 232-248 mOsmol kg from rainbow trout, with little variation over the spawning period. Protein concentration in carp seminal plasma was stable throughout the reproductive season, whereas the highest protein level (2.1 ± 0.3 mg mL ) in rainbow trout was observed in the middle phase of the spawning period. Seminal plasma composition in both species was analyzed using two-dimensional polyacrylamide gel electrophoresis combined with mass spectrometry. Thirteen differentially expressed protein spots in carp seminal plasma and sixteen spots in rainbow trout seminal plasma varied over the course of the reproductive season. The unique proteins identified are involved in the regulation of spermatogenesis, sperm motility, maintenance of sperm membrane lipid stability, and antioxidant protection. These results provide a deeper understanding of the role of fish seminal plasma proteins as well as a list of novel markers of sperm quality. Mol. Reprod. Dev. 83: 968-982, 2016 © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 89%

Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Shaliutina‐Kolešová

Kotas

Štěrba

et al. 2016

Molecular Reproduction Devel

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“…Similarly to rainbow trout, the quantitative comparison of proteome profile between SP and BP performed using 2D‐DIGE revealed that the majority of carp SP proteins are also present in BP . There is substantial overlap in the composition of the major proteins of carp and rainbow trout, suggesting that the general protective mechanisms in teleosts are well conserved.…”

Section: Discussionmentioning

confidence: 99%

“…The apparent duplication of many of the teleost genes contributes to the complexity of the fish immune system. So far, LC‐MS/MS has been applied to carp BP and has led to the identification of 88 plasma proteins . Although studies leading to the identification of a few or several blood proteins have been undertaken in some salmonid species , there is no comprehensive portrait of the salmonid BP proteome, which is needed to understand the molecular mechanisms taking place in the blood, particularly those related to immune reactions.…”

Section: Introductionmentioning

confidence: 99%

Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins

et al. 2017

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The characterisation of fish blood proteomes is important for comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study, LC-MS/MS and 2D-DIGE were applied to compare rainbow trout seminal (SP) and blood plasma (BP) proteomes. The 54 differentially abundant proteins identified in SP are involved in a variety of signalling pathways, including protein ubiquitination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoid X receptor activation, cell cycle and acute phase signalling. These findings may indicate the prevalence of acute phase signalling pathways in trout SP, and its essential role in protecting spermatozoa and reproductive tissues. Our study provides the first in-depth analysis of the trout BP proteome, with a total of 119 proteins identified. The major proteins of rainbow trout BP were recognised as acute phase proteins. Analysis of BP proteins indicated that acute phase response signalling, the complement system, liver X receptor/retinoid X receptor and farnesoid X receptor activation and the coagulation system are the top canonical pathways. This study enhances knowledge of the blood origin of trout SP proteins and understanding of fish reproductive biology. Our results provide new insight into blood proteins specifically important for fish physiology and innate immunity. The mass spectrometry data are available via ProteomeXchange with the identifier PXD005988 and https://doi.org/10.6019/PXD005988.

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“…Mass spectrometric experiments were performed using a nano LC LTQ-Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with a nano-electrospray ion source and an Easy II nano LC, as described previously with some modifications28. Animal horn samples were divided into soluble and insoluble fractions as described above, and 5 μL samples (1 μg of total protein) were loaded onto a self-packed 5 μm Reprosil C18AQ column (75 μm × 150 mm).…”

Section: Methodsmentioning

confidence: 99%

“…All MS/MS samples were analyzed using X!Tandem (thegpm.org; version TORNADO 2010.01.01.4) which was set up to search the bovidae_uniprot-taxonomy_9895 database (downloaded on the 26 th April, 2014). Spectra were also searched against an equal number of decoy sequences to estimate the false discovery rate (FDR) as described previously28. The specified enzyme was trypsin and up to two missed cleavages were allowed.…”

Section: Methodsmentioning

confidence: 99%

Available sustainable alternatives replace endangered animal horn based on their proteomic analysis and bio-effect evaluation

Liu¹,

Wang

Huang

et al. 2016

Sci Rep

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The use of endangered animal products in traditional Chinese medicine (TCM) and other ethno-medicines is culturally widespread across many regions of Asia. In the present study, traditional efficacies of seven types of animal horn including antipyretic, sedative and procoagulant activities were evaluated. Shotgun proteomic analysis was performed on material from horns following separation into soluble and insoluble fractions. Over 200 proteins were identified in each sample using nano LC-MS/MS, and these were classified according to their molecular function and cellular component using principal component analysis (PCA). The results indicated that seven horns showed antipyretic, sedative and procoagulant effect. Proteomic analysis showed that YH and WBH were similar to RH in terms of protein profile, and GH was similar to SAH. In addition, YH and GH were similar to RH in their cellular component classification profile. PCA based on the composition of keratin and keratin-associated proteins showed that constituents of WBH and GH were similar to RH and SAH, respectively. This is the first analysis of the protein content of animal horns used in TCM, and it is effective to substitute the horn of endangered animals with sustainable alternatives from domestic animals.

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Characterization of carp seminal plasma proteome in relation to blood plasma

Cited by 57 publications

References 46 publications

Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins

Available sustainable alternatives replace endangered animal horn based on their proteomic analysis and bio-effect evaluation

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