Characterization of Cannabis sativa allergens

Nayak, Ajay P.; Green, Brett J.; Sussman, Gordon; Berlin, Noam; Lata, Hemant; Chandra, Suman; ElSohly, Mahmoud A.; Hettick, Justin M.; Beezhold, Donald H.

doi:10.1016/j.anai.2013.04.018

Cited by 80 publications

(66 citation statements)

References 40 publications

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“…73–76 kDa IgE-reactive protein bands in cashew and pistachio nut. Rubisco has been suggested as an allergen in spinach, tomato, and cannabis [47, 48], and additional putative BiP allergens have been identified in cannabis seed [48] and chickpea [49]. The clinical relevance of IgE-reactive rubisco and/or BiPs proteins for a cashew or pistachio nut allergy, also in relation to cross-reactivity towards tree nuts and stability during heat processing, has yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

IgE Cross-Reactivity of Cashew Nut Allergens

Bastiaan‐Net

Reitsma

Cordewener

et al. 2018

Int Arch Allergy Immunol

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Background: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. Objectives: Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew. We aimed to determine IgE cross-sensitisation and cross-reactivity profiles in cashew nut-sensitised subjects, towards botanically related proteins of other Anacardiaceae family members and related tree nut species. Method: Sera from children with a suspected cashew nut allergy (n = 56) were assessed for IgE sensitisation to common tree nuts, mango, pink peppercorn, and sumac using dot blot technique. Allergen cross-reactivity patterns between Anacardiaceae species were subsequently examined by SDS-PAGE and immunoblot inhibition, and IgE-reactive allergens were identified by LC-MS/MS. Results: From the 56 subjects analysed, 36 were positive on dot blot for cashew nut (63%). Of these, 50% were mono-sensitised to cashew nuts, 19% were co-sensitised to Anacardiaceae species, and 31% were co-sensitised to tree nuts. Subjects co-sensitised to Anacardiaceae species displayed a different allergen recognition pattern than subjects sensitised to common tree nuts. In pink peppercorn, putative albumin- and legumin-type seed storage proteins were found to cross-react with serum of cashew nut-sensitised subjects in vitro. In addition, a putative luminal binding protein was identified, which, among others, may be involved in cross-reactivity between several Anacardiaceae species. Conclusions: Results demonstrate the in vitro presence of IgE cross-sensitisation in children towards multiple Anacardiaceae species. In this study, putative novel allergens were identified in cashew, pistachio, and pink peppercorn, which may pose factors that underlie the observed cross-sensitivity to these species. The clinical relevance of this widespread cross-sensitisation is unknown.

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Section: Discussionmentioning

confidence: 99%

IgE Cross-Reactivity of Cashew Nut Allergens

Bastiaan‐Net

Reitsma

Cordewener

et al. 2018

Int Arch Allergy Immunol

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“…The oilcake remaining after oil is expressed from the seeds is employed as a very nutritious feed supplement for livestock, but it also has potential for production of a high-protein flour. Proteins are potential allergens, but human allergies to hemp protein have rarely been reported (Nayak et al, 2013).…”

Section: Nutritional Aspects Of Hempseed Oilmentioning

confidence: 99%

Evolution and Classification of Cannabis sativa (Marijuana, Hemp) in Relation to Human Utilization

2015

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Cannabis sativa has been employed for thousands of years, primarily as a source of a stem fiber (both the plant and the fiber termed "hemp") and a resinous intoxicant (the plant and its drug preparations commonly termed "marijuana"). Studies of relationships among various groups of domesticated forms of the species and wildgrowing plants have led to conflicting evolutionary interpretations and different classifications, including splitting C. sativa into several alleged species. This review examines the evolving ways Cannabis has been used from ancient times to the present, and how human selection has altered the morphology, chemistry, distribution and ecology of domesticated forms by comparison with related wild plants. Special attention is given to classification, since this has been extremely contentious, and is a key to understanding, exploiting and controlling the plant. Differences that have been used to recognize cultivated groups within Cannabis are the results of disruptive selection for characteristics selected by humans. Wild-growing plants, insofar as has been determined, are either escapes from domesticated forms or the results of thousands of years of widespread genetic exchange with domesticated plants, making it impossible to determine if unaltered primeval or ancestral populations still exist. The conflicting approaches to classifying and naming plants with such interacting domesticated and wild forms are examined. It is recommended that Cannabis sativa be recognized as a single species, within which there is a narcotic subspecies with both domesticated and ruderal varieties, and similarly a non-narcotic subspecies with both domesticated and ruderal varieties. An alternative approach consistent with the international code of nomenclature for cultivated plants is proposed, recognizing six groups: two composed of essentially non-narcotic fiber and oilseed cultivars as well as an additional group composed of their hybrids; and two composed of narcotic strains as well as an additional group composed of their hybrids.

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“…15,18,29e33,42 Differences in source material and extraction techniques can introduce significant variability. Contaminants, additives, and inherent variability in the Table 2 Potential allergens of Cannabis sativa Delta-9-tetrahydrocannabinol (THC) 17,18 Nonspecific lipid transfer protein 7,29,30,38 (Can s 3) 31 Thaumatin-like protein 7 Ribulose-1,5-biphosphonate carboxylase/oxygenase (RuBisCO) 55 Oxygen-evolving enhancer protein 2 55 native allergen extracts might lead to irrelevant IgE binding components that can cloud diagnostic evaluation. 56 Some studies have demonstrated skin test reaction positivity in atopic controls and those without prior exposure or clinical symptoms, thus emphasizing that further study is needed to establish skin test sensitivity and specificity.…”

Section: Diagnosismentioning

confidence: 99%