Characterization of Candida albicans mannan-induced, mannan-specific delayed hypersensitivity suppressor cells

Abstract

We have shown previously that CBA/J mice immunized with Candida albicans developed delayed hypersensitivity (DH) demonstrable with mannan (MAN) extracted from the same organism and that the intravenous (i.v.) injection of MAN prior to or during the immunization phase resulted in the suppression of the MAN-specific DH response. In this study, we demonstrate that MAN-induced suppression of DH is a T-lymphocyte-mediated phenomenon. Suppressor cells induced in vivo by the i.v. injection of MAN into naive mice 1 to 7 days prior to harvest were passaged through nylon wool, treated with various surface-specific antibodies and complement, and then injected i.v. into immunized syngeneic recipients. Enrichment of splenic T cells by passage over nylon wool and transfer of the nylon-wool-nonadherent populations to immunized recipient mice suppressed DH in a dose-dependent manner. Depletion of Thy' or Lyt-2+ cells from nylon-wool-nonadherent populations regularly ablated the ability of such suspensions to transfer suppression. Treatment of the same transfer suspensions with anti-Lyt-1 had variable effects, suggesting that the surface density of the Lyt-1 antigen was not as constant from population to population as was the Lyt-2 antigen. In addition, C. albicans MAN-induced suppressor cells were able to suppress DH demonstrable with Candida tropicalis MAN in animals immunized with C. tropicalis. Suppression of DH by MAN in this model, therefore, is mediated by Thy' Lyt-2+ lymphocytes.

Neutralizing antibody to interleukin 4 induces systemic protection and T helper type 1-associated immunity in murine candidiasis.

“…In addition, both specific and nonspecific modulation of immune responses have been reported as a consequence of C. albicans infection in mice. Garner et al (23) showed that mannan administered intravenously to naive mice induced a population of suppressor T lymphocytes that, when transferred to immunized mice, suppressed antigen-specific DTH responses. Ashman (24) suggested that active downregulation of protective immunity might occur in mice that are genetically susceptible to candidiasis and are characterized by high antibody levels and poor DTH responses.…”

“…Values represent the means _+ SEM of three experiments. Specific antibody levels in uninfected mice were negligible or undetectable 23. Romani et al…”

Effects of preinduced Candida-specific systemic cell-mediated immunity on experimental vaginal candidiasis

“…We reasoned that Candida-specific Ts cells would be a useful tool to study vaginal candidiasis under reduced Candida-specific CMI. The Ts cell approach was also relevant since Ts cells characterized both in patients with chronic mucocutaneous candidiasis (9, 35) and in murine models of cutaneous candidiasis (8,17) have been implicated in the development of Candida infections. Indeed, the presence of Candida-specific Ts cells at the time of vaginal inoculation effectively reduced the subsequent vaginal infection-derived systemic CMI.…”

Candida-specific cell-mediated immunity is demonstrable in mice with experimental vaginal candidiasis

“…In contrast, the importance of CMI in gastrointestinal host resistance to C. albicans has been demonstrated in both humans (4,11,25) and animal models (2, 6-8, 12, 30). The use of murine models has allowed the identification of both lymphocyte populations that protect against Candida gastrointestinal infection (7,8,12) and those that down-regulate Candida-specific CMI, thus creating susceptibility to infection (3,17). Additionally, athymic (2, 6) and severe combined immunodeficient (Tand B-cell-deficient) (30) mice have been particularly useful for studying susceptibility to gastrointestinal candidiasis.…”