Characterization of "Campylobacter pyloridis" by culture, enzymatic profile, and protein content

Abstract

Campylobacter pyloridis" has been recently described as a gastritis-associated bacterium. We studied 20 strains. The bacteria had most of the characteristics of Campylobacter spp. strains. They were hippurate negative and tolerant to triphenyl tetrazolium chloride (at 0.4 and 1 mg/ml). They grew on all the media commonly used in laboratories, although chocolate agar was the most effective for isolation. They grew in a microaerophilic atmosphere as well as in an atmosphere enriched in C02 and when incubated at 37°C but not at 30 or 42°C. A total of 31 enzymes were present among the 78 studied. y-Glutamyl-transpeptidase activity was, in addition to urea hydrolysis, an interesting feature for the identification of these bacteria. The protein profiles of the 20 strains were similar.

Helicobacter pylori: A Fickle Germ

“…pylori BM46 was the clinical isolate used for this work. Strain, obtained from patient who underwent endoscopy with gastroduodenal disease, was identified by standard methods (7,18). H. pylori NCTC 11639 was used as a standard control strain.…”

Novel Genotypes in Helicobacter pylori Involving Domain V of the 23S rRNA Gene

“…Fresh and frozen homogenates of biopsies were streaked onto clarithromycin-free Trypticase Soy agar plates supplemented with 5% horse serum, culture supplement Vitox (Oxoid, Barsingstoke, Hampshire, England), and antibiotic supplement Dent (Oxoid) (TSA agar) [13] and incubated for 3 -5 days. H. pylori colonies were identified by standard methods [14]. To evaluate antibiotic resistance, 10 colonies from the resulting growing cells were randomly selected and streaked onto TSA agar plates to grow.…”

An Alternative Non‐Macrolide, Non‐Imidazole Treatment Regimen for Curing Helicobacter pylori and Duodenal Ulcers: Ranitidine Bismuth Citrate Plus Amoxicillin

“…All samples were assigned a unique random number and were sent to a central laboratory where H. pylori detection tests were evaluated in a double-blind fashion by laboratory personnel. The presence or absence of H. pylori infection was determined by assessment of the gastric antral biopsies for culture growth [26], and the results of CLOtests and histological studies. Two of the four antral biopsies were used for the CLOtest and culture.…”