2023
DOI: 10.3389/fmicb.2023.1247211
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Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios

Annika Dreyer,
Christof Lenz,
Uwe Groß
et al.

Abstract: In dynamic microbial ecosystems, bacterial communication is a relevant mechanism for interactions between different microbial species. When C. jejuni resides in the intestine of either avian or human hosts, it is exposed to diverse bacteria from the microbiome. This study aimed to reveal the influence of co-incubation with Enterococcus faecalis, Enterococcus faecium, or Staphylococcus aureus on the proteome of C. jejuni 81–176 using data-independent-acquisition mass spectrometry (DIA-MS). We compared the prote… Show more

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Cited by 1 publication
(2 citation statements)
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“…While Chen et al achieved a coverage of approximately 35% in their analysis of the proteome profiles of C. jejuni under peracetic acid exposure [32], the coverage was approximately 61% in the analysis of deoxycholate-induced stress by Man et al [33]. Our analysis, with a coverage of 58.9%, shows a similarly high coverage [18], which we were able to increase to 83.6% using a modernized technique [34].…”
Section: Discussionmentioning
confidence: 54%
See 1 more Smart Citation
“…While Chen et al achieved a coverage of approximately 35% in their analysis of the proteome profiles of C. jejuni under peracetic acid exposure [32], the coverage was approximately 61% in the analysis of deoxycholate-induced stress by Man et al [33]. Our analysis, with a coverage of 58.9%, shows a similarly high coverage [18], which we were able to increase to 83.6% using a modernized technique [34].…”
Section: Discussionmentioning
confidence: 54%
“…In the studies of category B, we often observe a significantly lower coverage rate of the measured or quantified proteome compared to the predicted proteome. Großeholz et al analyzed the pH adaptation of Enterococcus faecalis at approximately 53% coverage rate [25], and in a recent study of our group examining the bile acid response of E. faecalis and Enterococcus faecium the coverage rates were 43.5% and 45.8% [26], while Kang et al studied the stress response of Methicillin-resistant Staphylococcus aureus to lactobionic acid at approximately 47% coverage rate [27]. Furthermore, other studies targeting specific protein fractions, such as membrane proteins or cytosolic proteins, using different pre-separation methods, cannot be compared here as the coverage is inherently less than 10% due to the method used.…”
Section: Discussionmentioning
confidence: 99%