Characterization of calcium signaling proteins from the fat body of the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae): Implications for diapause and lipid metabolism

Doğan, Cansu; Hänniger, Sabine; Heckel, David G.; Coutu, Cathy; Hegedus, Dwayne D.; Crubaugh, Linda K.; Groves, Russell L.; Mutlu, Damla Amutkan; Suludere, Zekiye; Bayram, Şerife; Toprak, Umut

doi:10.1016/j.ibmb.2021.103549

Cited by 16 publications

(7 citation statements)

References 119 publications

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“…In 2021, Dogan et al investigated the key binding proteins, finding calmodulin, calcineurin, and regucalcin in the Leptinotarsa decemlineata fat body. This indicates additional adaptation of this tissue to lipid metabolism [73].…”

Section: Lipid Metabolism Under Various Environmental Conditionsmentioning

confidence: 80%

Fat Body—Multifunctional Insect Tissue

Skowronek

Wójcik

Strachecka

2021

Insects

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The biodiversity of useful organisms, e.g., insects, decreases due to many environmental factors and increasing anthropopressure. Multifunctional tissues, such as the fat body, are key elements in the proper functioning of invertebrate organisms and resistance factors. The fat body is the center of metabolism, integrating signals, controlling molting and metamorphosis, and synthesizing hormones that control the functioning of the whole body and the synthesis of immune system proteins. In fat body cells, lipids, carbohydrates and proteins are the substrates and products of many pathways that can be used for energy production, accumulate as reserves, and mobilize at the appropriate stage of life (diapause, metamorphosis, flight), determining the survival of an individual. The fat body is the main tissue responsible for innate and acquired humoral immunity. The tissue produces bactericidal proteins and polypeptides, i.e., lysozyme. The fat body is also important in the early stages of an insect’s life due to the production of vitellogenin, the yolk protein needed for the development of oocytes. Although a lot of information is available on its structure and biochemistry, the fat body is an interesting research topic on which much is still to be discovered.

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Section: Lipid Metabolism Under Various Environmental Conditionsmentioning

confidence: 80%

Fat Body—Multifunctional Insect Tissue

Skowronek

Wójcik

Strachecka

2021

Insects

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“…Specifically, the decreases were around 14 and 12% in third and fifth instar larvae, respectively. Various studies in mammals and insects revealed the involvement of dietary Ca 2+ and specific Ca 2+ signaling genes in lipid metabolism, leading to lipolysis ( Allen and Beck, 1986 ; Shi et al, 2001 ; Zemel, 2002 ; Jacqmain et al, 2003 ; Arruda and Hotamisligil, 2015 ; Maus et al, 2017 ; Doğan et al, 2021a , b ; Toprak et al, 2021 ). In Drosophila, elevated cytosolic Ca 2+ levels lead to activation of lipolysis ( Subramanian et al, 2013a , b ; Baumbach et al, 2014a , b ; Bi et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…HFD, HSD, a calcium-rich diet (CRD), and a plant-based diet (PBD) were supplied throughout the larval development and compared to a standard artificial bean diet. The CRD was included as studies have indicated the involvement of Ca 2+ signaling molecules, cellular, and dietary Ca 2+ in lipid and carbohydrate metabolism, body weight regulation, obesity, and diabetes in insects and mammals ( Zemel et al, 2000 ; Shi et al, 2001 ; Zemel, 2002 ; Jacqmain et al, 2003 ; Baumbach et al, 2014a , b ; Arruda and Hotamisligil, 2015 ; Maus et al, 2017 ; Alomaim et al, 2019 ; Doğan et al, 2021a , b ; Toprak et al, 2021 ). The PBD (lettuce leaves) was used as lettuce is a natural host for S .…”

Section: Introductionmentioning

confidence: 99%

What You Eat Matters: Nutrient Inputs Alter the Metabolism and Neuropeptide Expression in Egyptian Cotton Leaf Worm, Spodoptera littoralis (Lepidoptera: Noctuidae)

et al. 2021

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Graphical AbstractThe parameters examined in Spodoptera littoralis larvae in response to four different diets, high-fat, high-sugar, calcium-rich and plant-based are shown starting with the developmental duration, weight, triglyceride and trehalose levels, lipid droplet structure, and finally with expression of four neuropeptide genes encoding adipokinetic hormone (AKH), insulin-like peptide 1 (ILP1), insulin-like peptide 2 (ILP2) and short neuropeptide F (sNPF).

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“…LdABCB1 and LdCAD were silenced via RNAi (Doğan et al, 2021; Güney et al, 2021) to examine the involvement of these genes in the Cry3Aa mode of action. The dsRNAs specific to a region of 461 and 470 bp in LdABCB1 and LdCAD , respectively, were amplified using the T7 RiboMAX Express RNAi System (Promega) and the corresponding dsFP‐RP primers (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Silencing of an ABC transporter, but not a cadherin, decreases the susceptibility of Colorado potato beetle larvae to Bacillus thuringiensis ssp. tenebrionis Cry3Aa toxin

Güney

Cedden

Hänniger

et al. 2021

Arch Insect Biochem Physiol

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The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae), is a major pest of potato plants worldwide and is notorious for its ability to develop resistance to insecticides. Cry3 toxins synthesized by Bacillus thuringiensis ssp. tenebrionis have been used successfully to manage this pest. Resistance to Cry toxins is a concerning problem for many insect pests; therefore, it is important to determine the mechanisms by which insects acquire resistance to these toxins. Cadherin‐like and ABC transporter proteins have been implicated in the mode of action of Cry toxins as mutations in these genes render lepidopterans resistant to them; however, clear consensus does not exist on whether these proteins also play a role in Cry3 toxin activity and/or development of resistance in coleopterans. In the current study, we identified the L. decemlineata orthologues of the cadherin (LdCAD) and the ABCB transporter (LdABCB1) that have been implicated in the mode of action of Cry toxins in other coleopterans. Suppression of LdABCB1 via RNA interference reduced toxin‐related larval mortality, whereas partial silencing of LdCAD did not. Our results suggest that the ABCB is involved in the mode of action of Cry3Aa toxins; however, no evidence was found to support the role of cadherin as a receptor of Cry3Aa in L. decemlineata.

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Characterization of calcium signaling proteins from the fat body of the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae): Implications for diapause and lipid metabolism

Cited by 16 publications

References 119 publications

Fat Body—Multifunctional Insect Tissue

Fat Body—Multifunctional Insect Tissue

What You Eat Matters: Nutrient Inputs Alter the Metabolism and Neuropeptide Expression in Egyptian Cotton Leaf Worm, Spodoptera littoralis (Lepidoptera: Noctuidae)

Silencing of an ABC transporter, but not a cadherin, decreases the susceptibility of Colorado potato beetle larvae to Bacillus thuringiensis ssp. tenebrionis Cry3Aa toxin

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