A nano-magnetic catalyst CaO/NaY-Fe 3 O 4 was synthesized and used as a catalyst for biodiesel production from canola oil. The structure of the synthesized catalysts was characterized using several methods such as XRD, FESEM, BET, FT-IR and VSM. In order to optimize the influence of the operating variables, such as methanol to canola oil molar ratio, the amount of catalyst and reaction time on the yield of transesterification reaction, an experimental design was applied based on the Box-Behnken method. The optimum values of these variables were predicted by the Cubic model. Maximum amount of methyl esters (95.37%) was achieved under the optimum operating conditions and it was in excellent agreement with the experimental result within an error less than 5%.