Characterization of cadmium-responsive transcription factors in wolf spider Pardosa pseudoannulata

Wang, Juan; He, Yu; Peng, Xian‐Jin; Lv, Bo; Wang, Zhi; Song, Qisheng

doi:10.1016/j.chemosphere.2020.129239

Cited by 18 publications

(6 citation statements)

References 42 publications

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“…It has been observed that spiders exhibit distinct responses to the toxicities of heavy metals by modulating DEGs and DEPs expression associated with protein synthesis, energy supply, antioxidant mechanisms, and neural regulation. 32,36,49,61,73 Through the utilization of an integrated multidisciplinary research approach, our study proposed that the toxicities of Cd and Pb combined exposure, but not limited to oxidative damage, degenerative changes of cell, and disrupting of neuromodulation and amino acid anabolic, could subsequently lead to impeding the silk production and web-weaving capabilities of spiders. Based on the transcriptomics findings, it could be inferred that the observed downregulation of genes involved in protein synthesis and alteration of spidroin genes as well as the upregulation of AChEs and Glu genes potentially tend to contribute to the inhibition of silk protein synthesis and disruption of neuromodulation in web-weaving behavior, respectively.…”

Section: Potential Mechanism Of CD and Pb Combined Exposure On Silk P...mentioning

confidence: 99%

Transcriptome Changes Reveal the Toxic Mechanism of Cadmium and Lead Combined Exposure on Silk Production and Web-Weaving Behavior of Spider A. ventricosus

Yang,

Wang

et al. 2023

Environ. Sci. Technol.

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The combined exposure of multiple metals imposes a substantial burden on the ecophysiological functions in organisms; however, the precise mechanism(s) remains largely unknown. Here, adult female A. ventricosus were exposed to single and combined exposure to cadmium (Cd) and lead (Pb) through the food chain. The aim was to explore the combined toxicity of these metals on silk production and web-weaving behavior at physiological, cellular morphological, and transcriptomic levels. The Cd and Pb combined exposure significantly inhibited the ability of silk production and web-weaving, including reduced silk fiber weight and diameter of single strands, lowered weaving position, induced nocturnal weaving, and increased instances of no-web, and showed a dose–response relationship on the Cd and Pb bioaccumulation. Concurrently, severe oxidative stress and degenerative changes in cells were observed. In addition, the combined pollution of Cd and Pb demonstrated synergistic effects, influenced by variations in concentration, on the enrichment of metals, inhibition of silk weight, oxidative damage, and cellular degeneration. At the transcriptome level, the upregulated ampullate spidroin genes and downregulated amino acid anabolic genes, upregulated Far genes and downregulated cytoskeleton-related TUBA genes, and overexpressed AChE and Glu genes may tend to present promising potential as biomarkers for silk protein synthesis, cellular degeneration, and neurotransmitter induction. This study offers an enormous capability for a comprehensive understanding of the eco-toxicological effects and mechanisms of multiheavy metals pollution.

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Section: Potential Mechanism Of CD and Pb Combined Exposure On Silk P...mentioning

confidence: 99%

Transcriptome Changes Reveal the Toxic Mechanism of Cadmium and Lead Combined Exposure on Silk Production and Web-Weaving Behavior of Spider A. ventricosus

Yang,

Wang

et al. 2023

Environ. Sci. Technol.

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“…Alternatively, peroxiredoxins are also the major cellular enzymatic scavengers that control H 2 O 2 level, which are known gene targets of FOXO3a. The up-regulation of these antioxidant enzymes can reduce cellular ROS production, consequently ameliorating oxidative injury, and raise cellular survival in the stressed liver [ 98 , 118 , 119 , 120 , 121 ]. In this respect, a better understanding is necessary for the function of FOXO3a in mediating nuclear–mitochondrial crosstalk because they can influence each other’s activities, such as oxidative stress response.…”

Section: Role Of Foxo3a In the Livermentioning

confidence: 99%

Role of FOXO3a Transcription Factor in the Regulation of Liver Oxidative Injury

Jin

Zhang

et al. 2022

Antioxidants

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Oxidative stress has been identified as a key mechanism in liver damage caused by various chemicals. The transcription factor FOXO3a has emerged as a critical regulator of redox imbalance. Multiple post-translational changes and epigenetic processes closely regulate the activity of FOXO3a, resulting in synergistic or competing impacts on its subcellular localization, stability, protein–protein interactions, DNA binding affinity, and transcriptional programs. Depending on the chemical nature and subcellular context, the oxidative-stress-mediated activation of FOXO3a can induce multiple transcriptional programs that play crucial roles in oxidative injury to the liver by chemicals. Here, we mainly review the role of FOXO3a in coordinating programs of genes that are essential for cellular homeostasis, with an emphasis on exploring the regulatory mechanisms and potential application of FOXO3a as a therapeutic target to prevent and treat liver oxidative injury.

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“…The wolf spider Pardosa pseudoannulata (Araneae: Lycosidae), which is a hunting spider, 30 mainly feeds on rice pests such as planthoppers, leafhoppers, and other Lepidoptera insects. [31][32][33] It has strong adaptability to the environment and is widely distributed, [34][35][36][37][38] generally being found in wet habitats near the edge of water bodies. 39 Research has shown that the vitellogenin receptor gene 38 and the functional ecdysteroid Ponasterone A (PA) 40 are vital to the growth and development of Pardosa pseudoannulata.…”

Section: Introductionmentioning

confidence: 99%

RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata

Li,

Zhang

et al. 2023

Pest Management Science

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BACKGROUNDInsulin signalling pathways play crucial roles in regulating growth and development in insects, but their effects on the growth and development of Arachnids, such as spiders, have rarely been studied. As a valuable pest natural enemy in agricultural fields, the molecular mechanisms of insulin signalling pathway‐mediated growth and development of the wolf spider, Pardosa pseudoannulata, are of particular interest.RESULTSIn this study, we identified and characterized six insulin signalling pathway genes—InR, InR2, IRS1, PI3K1, PI3K2, and PDK—in P. pseudoannulata. Real‐time quantitative PCR results were used to analyse the relative expression levels of the six genes in different developmental instars and tissues, and in response to starvation treatment. In addition, the function of the insulin receptor substrate (IRS1) gene was investigated using RNA interference technology, which found that IRS1 significantly influenced nutrient content, developmental duration, body weight, and gonad development.CONCLUSIONThis study revealed the roles of six key insulin signalling pathway genes in P. pseudoannulata, and in particular the importance of the IRS1 gene in regulating growth and development in the spider. The results lay the foundation for further research on the internal regulation mechanisms of growth and development in Araneae species, and also provide a reference for the artificial breeding of spiders.This article is protected by copyright. All rights reserved.

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Characterization of cadmium-responsive transcription factors in wolf spider Pardosa pseudoannulata

Cited by 18 publications

References 42 publications

Transcriptome Changes Reveal the Toxic Mechanism of Cadmium and Lead Combined Exposure on Silk Production and Web-Weaving Behavior of Spider A. ventricosus

Transcriptome Changes Reveal the Toxic Mechanism of Cadmium and Lead Combined Exposure on Silk Production and Web-Weaving Behavior of Spider A. ventricosus

Role of FOXO3a Transcription Factor in the Regulation of Liver Oxidative Injury

RNA interference against the putative insulin receptor substrate gene IRS1 affects growth and development in the pest natural enemy Pardosa pseudoannulata

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