Characterization of C-, J- and V-region-genes of the feline T-cell receptor γ

Clonality Testing in Veterinary Medicine

“…The rearrangement of antigen receptor Improvement of clonality assays subsequently focused on the identification of additional genes to increase primer coverage. Weiss et al [55][56][57] and Henrich et al 18 developed clonality assays targeting the feline TRG and IGH locus, respectively. Their assays were based on sequence data obtained from diligent mining of the TRACE archive and sequencing of cDNA clones.…”

Feline T-Cell Receptor V- and J-Region Sequences Retrieved from the Trace Archive and from Transcriptome Analysis of Cats

“…A BLAST search of the Trace Archive yielded four previously unidentified V-region genes designated as fTRGV5P ($\underset{¯}{\text{gnl}|\text{ti}|663092402}$), -4.1P ($\underset{¯}{\text{gnl}|\text{ti}|838072588}$, $\underset{¯}{\text{gnl}|\text{ti}|827924706)}$, -4.2P ($\underset{¯}{\text{gnl}|\text{ti}|643947952}$, $\underset{¯}{\text{gnl}|\text{ti}|646819523}$, $\underset{¯}{\text{gnl}|\text{ti}|644534307}$), and -4.3P ($\underset{¯}{\text{gnl}|\text{ti}|631627457}$, $\underset{¯}{\text{gnl}|\text{ti}|630610594}$, $\underset{¯}{\text{gnl}|\text{ti}|2159892447}$) (see Figures 1(a) and 1(b) and one potential J-region gene designated as fTRGJ2.3 (see Figure 2(a) besides those already described [8, 9]. One further J-region gene (fTRGJ2.4, see Figures 2(a) and 3) was identified in one clone (fTGII.2) amplified by the SMART RACE procedure described above.…”

“…Previously generated J-region and V-region sequences [9] were used to search the Trace Archive of feline genomic sequences (NCBI, Bethesda, USA; http://www.ncbi.nlm.nih.gov/Traces/) employing the BLAST Search algorithm (NCBI, Bethesda, USA; http://www.ncbi.nlm.nih.gov/blast/). Sequence analyses were carried out using ClustalW (EMBL-EBI, Heidelberg, Germany; http://www.ebi.ac.uk/Tools/clustalw/) and V-Quest software (IMGT, Montpellier, France; http://imgt.cines.fr/IMGT_vquest/share/textes/ [16]).…”

“…In the second round we used 70°C with a touch-down of 0.5°C over 12 cycles followed by 64°C for 24 cycles. DNA was handled, cloned, and sequenced as described [9]. Sequences containing a CAN-SINE and one particular sequence containing a new fTRGJ-variant were submitted to EMBL Nucleotide Sequence Database: AM502837, AM502838, AM502846, and AM747390 (fTGII.2).…”

“…In the feline TRG repertoire four different V-region genes (fTRGV1 – 4), eight different J-region genes (fTRGJ1.1 to -1.5, fTRGJ2.1 and -2.2, fTRGJ3), and six different C-region genes (fTRGC1 to -6) have already been described [8, 9]. Compared to the known human TRG repertoire fewer V-region genes are known in the cat (4) than in humans (12– 15) [7, 10].…”