Characterization of Bt Transgenic Plants: A Review

Keshavareddy, G.; Kumar, Arun

doi:10.20546/ijcmas.2018.702.368

Cited by 3 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular gene stacking or simultaneous gene pyramiding is another strategy where a few genes are introduced at the same time in a plant using transgenic approaches such as Agrobacterium‐mediated transformation or by biolistic methods (Keshavareddy & Kumar, 2018; Srivastava et al, 2017). The molecular gene stacking can be achieved in plants via two routes: co‐transformation or re‐transformation, for which the details are presented below.…”

Section: Introductionmentioning

confidence: 99%

Stacking for future: Pyramiding genes to improve drought and salinity tolerance in rice

Shailani

Joshi

Singla‐Pareek

et al. 2020

Physiologia Plantarum

View full text Add to dashboard Cite

Abiotic stresses, such as drought and salinity, adversely affect rice production and cause a severe threat to food security. Conventional crop breeding techniques alone are inadequate for achieving drought stress tolerance in crop plants. Using transgenic technology, incremental improvements in tolerance to drought and salinity have been successfully attained via manipulation of gene(s) in several crop species. However, achieving the goal via pyramiding multiple genes from the same or different tolerance mechanisms has received little attention. Pyramiding of multiple genes can be achieved either through breeding, by using marker-assisted selection, or by genetic engineering through molecular stacking co-transformation or re-transformation. Transgene stacking into a single locus has added advantages over breeding or re-transformation since the former assures co-inheritance of genes, contributing to more effective tolerance in transgenic plants for generations. Drought, being a polygenic trait, the potential candidate genes for gene stacking are those contributing to cellular detoxification, osmolyte accumulation, antioxidant machinery, and signaling pathways. Since cellular dehydration is inbuilt in salinity stress, manipulation of these genes results in improving tolerance to salinity along with drought in most of the cases. In this review, attempts have been made to provide a critical assessment of transgenic plants developed through transgene stacking and approaches to achieve the same. Identification and functional validation of more such candidate genes is needed for research programs targeting the gene stacking for developing crop plants with high precision in the shortest possible time to ensure sustainable crop productivity under marginal lands. | INTRODUCTIONRice is a staple food crop consumed by nearly half of the human population. Asia alone contributes to 90% of the rice produced worldwide (Maclean et al., 2002). However, changing climatic conditions, biotic stress (such as fungi, bacteria, insects, viruses), and abiotic stress (including salinity, drought, submergence, extreme temperature, wounding, mineral deficiency, and oxidative stress) affect rice plants in many ways, ultimately contributing to reduction in crop yield (Akos et al., 2019;Kaya et al., 2020). Among these abiotic stresses, drought alone affects more than 50% of the rice production (23 million ha of rain-fed rice globally)

show abstract

Section: Introductionmentioning

confidence: 99%