Characterization of brassinosteroid-regulated proteins in a nuclear-enriched fraction of Arabidopsis suspension-cultured cells

Shigeta, Tomoaki; Yasuda, Daisuke; Mori, Takafumi; Yoshimitsu, Yuya; Nakamura, Yasushi; Asami, Tadao; Okamoto, Shigehisa; Matsuo, Tomoaki

doi:10.1016/j.plaphy.2011.04.012

Cited by 12 publications

(10 citation statements)

References 38 publications

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“…Consistently, lack of SDG725 resulted in a reduced level of H3K36me2/3 in several BR-related genes, leading to the down-regulation of their expression (Sui et al, 2012). In a third study aiming at identifying BR-regulated proteins in nuclear-enriched fractions of Arabidopsis cells, several chromatin-remodeling proteins were identified, suggesting that BR-induced chromatin remodeling may be required for BR-regulated gene expression (Shigeta et al, 2011a(Shigeta et al, , 2011b.…”

Section: Regulation Of Br Signalingmentioning

confidence: 83%

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

Vriet¹,

Russinova²,

Reuzeau³

2013

Molecular Plant

154

132

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The plant steroid hormones, brassinosteroids (BRs), and their precursors, phytosterols, play major roles in plant growth, development, and stress tolerance. Here, we review the impressive progress made during recent years in elucidating the components of the sterol and BR metabolic and signaling pathways, and in understanding their mechanism of action in both model plants and crops, such as Arabidopsis and rice. We also discuss emerging insights into the regulations of these pathways, their interactions with other hormonal pathways and multiple environmental signals, and the putative nature of sterols as signaling molecules.

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Section: Regulation Of Br Signalingmentioning

confidence: 83%

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

Vriet¹,

Russinova²,

Reuzeau³

2013

Molecular Plant

154

132

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“…Moreover, H3K36 methylation was shown to regulate BR-related gene expression and plant development in rice (Oryza sativa; Sui et al, 2012). In addition, a proteomic study identified several BR-responsive nucleus-enriched proteins, including nucleosome assembly proteins, HISTONE DEACETY-LASE 2B, and VERNALIZATION INDEPENDENCE3, suggesting that BR-induced gene expression is regulated by chromatin remodeling (Shigeta et al, 2011a(Shigeta et al, , 2011b. Furthermore, a genomewide analysis in Arabidopsis of histone modifications during photomorphogenesis demonstrated that H3K27me3 is a major regulator of the GA metabolic pathway, because dioxygenase genes are highly targeted by H3K27me3 and the level of H3K27me3 modification is correlated with expression changes (Charron et al, 2009).…”

Section: Br and Ga Signaling Regulate Histone Modificationmentioning

confidence: 99%

The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis

et al. 2014

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Plant cell elongation is controlled by endogenous hormones, including brassinosteroid (BR) and gibberellin (GA), and by environmental factors, such as light/darkness. The molecular mechanisms underlying the convergence of these signals that govern cell growth remain largely unknown. We previously showed that the chromatin-remodeling factor PICKLE/ ENHANCED PHOTOMORPHOGENIC1 (PKL/EPP1) represses photomorphogenesis in Arabidopsis thaliana. Here, we demonstrated that PKL physically interacted with PHYTOCHROME-INTERACTING FACTOR3 (PIF3) and BRASSINAZOLE-RESISTANT1 (BZR1), key components of the light and BR signaling pathways, respectively. Also, this interaction promoted the association of PKL with cell elongation-related genes. We found that PKL, PIF3, and BZR1 coregulate skotomorphogenesis by repressing the trimethylation of histone H3 Lys-27 (H3K27me3) on target promoters. Moreover, DELLA proteins interacted with PKL and attenuated its binding ability. Strikingly, brassinolide and GA 3 inhibited H3K27me3 modification of histones associated with cell elongation-related loci in a BZR1-and DELLA-mediated manner, respectively. Our findings reveal that the PKL chromatin-remodeling factor acts as a critical node that integrates light/darkness, BR, and GA signals to epigenetically regulate plant growth and development. This work also provides a molecular framework by which hormone signals regulate histone modification in concert with light/dark environmental cues.

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“…To achieve this, quantitation of protein phosphorylation by quantitative phosphoproteomic methods is certainly important. Previous attempts to identify BR-induced protein phosphorylation by coupling gel electrophoresis with liquid chromatography/mass spectrometry (MS) have at best identified only a small number of phosphoproteins in Arabidopsis (Deng et al, 2007; Tang et al, 2008a; Shigeta et al, 2011). Until recently, novel phosphopeptide enrichment methods, advanced MS, and sophisticated algorithms have promoted the process of wide-scale phosphoprotein identification, especially in a quantitative manner.…”

Section: Introductionmentioning

confidence: 99%

A Quantitative Proteomic Analysis of Brassinosteroid-induced Protein Phosphorylation in Rice (Oryza sativa L.)

et al. 2017

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The group of polyhydroxysteroid phytohormones referred to as the brassinosteroids (BRs) is known to act on plant development and the stress response. BR signal transduction relies largely on protein phosphorylation. By employing a label-free, MS (Mass Spectrometry)-based phosphoproteomic approach, we report here the largest profiling of 4,034 phosphosites on 1,900 phosphoproteins from rice young seedlings and their dynamic response to BR. 1,821 proteins, including kinases, transcription factors and core components of BR and other hormone signaling pathways, were found to be differentially phosphorylated during the BR treatment. A Western blot analysis verified the differential phosphorylation of five of these proteins, implying that the MS-based phosphoproteomic data were robust. It is proposed that the dephosphorylation of gibberellin (GA) signaling components could represent an important mechanism for the BR-regulated antagonism to GA, and that BR influences the plant architecture of rice by regulating cellulose synthesis via phosphorylation.

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Characterization of brassinosteroid-regulated proteins in a nuclear-enriched fraction of Arabidopsis suspension-cultured cells

Cited by 12 publications

References 38 publications

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

From Squalene to Brassinolide: The Steroid Metabolic and Signaling Pathways across the Plant Kingdom

The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis

A Quantitative Proteomic Analysis of Brassinosteroid-induced Protein Phosphorylation in Rice (Oryza sativa L.)

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