Characterization of Boxwood Shoot Bacterial Communities and Potential Impact from Fungicide Treatments

Li, Xiaoping; Tseng, Hsien Tzer; Omolehin, Olanike; Taylor, Chad; Taylor, Amanda; Kong, Ping; Daughtrey, Margery; Gouker, Fred E.; Hong, Chuanxue

doi:10.1128/spectrum.04163-22

Cited by 3 publications

(7 citation statements)

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“…We discovered a wealth of fungal genera known as pathogens and saprophytes dominating the boxwood shoots. We also found that the predominant bacteria included many genera that have species and strains known to promote plant growth and health, agreeing with our previous study [ 10 ]. Foliar application of three film-forming anti-desiccants had more marked impact on fungi than bacteria in both community composition and structure, especially with Vapor Gard and Wilt-Pruf.…”

Section: Discussionsupporting

confidence: 93%

“…Diverse bacteria and fungi were identified from the two boxwood cultivars, extending our understanding of microbial assemblies in boxwood phyllosphere. Boxwood shoots were dominated by Proteobacteria at the phylum level and Methylobacterium at the genus level, consistent with our previous observation [ 10 ]. Many predominant bacterial genera, including Methylobacterium , Stenotrophomonas , Bacillus , Sphingomonas , and Burkholderia , have species and strains known to promote plant growth and health.…”

Section: Discussionsupporting

confidence: 92%

“…PCR amplification of full length 16 S rRNA amplicon followed the protocol in our previous study [ 10 ]. The extended ITS regions were amplified with the fungal-specific primers NSA3 and NLC2 [ 53 ].…”

Section: Methodsmentioning

confidence: 99%

“…They also isolated a Burkholderia SSG strain from boxwood leaf tissues and showed its beneficial activities in nutrient acquisition [ 3 ] and pathogen antagonism [ 9 ]. Recently, we revealed diverse bacterial communities on the boxwood shoot surface and in the internal tissue and found that canopy cover sprayed with contact and systemic fungicides had a broad and strong impact on the epiphytic bacterial communities [ 10 ]. These studies have advanced our knowledge of the biology of boxwood and also highlighted the importance of evaluating other agrochemicals for their potential impacts on the boxwood phyllosphere microbiota—an essential step to establishing a systems approach to utilize the full potential of microbiomes for better boxwood management.…”

Section: Introductionmentioning

confidence: 99%

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Boxwood phyllosphere fungal and bacterial communities and their differential responses to film-forming anti-desiccants

Li,

Omolehin,

Hemmings

et al. 2023

BMC Microbiol

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Background Anti-desiccant is a class of agrochemicals widely used to protect plants from water stresses, rapid temperature variations, heat and sunburn, frost and freeze damages, transplant shock, and pathogen and pest attack. Although anti-desiccants are generally considered non-toxic to organisms, it is unclear whether they may impact the phyllosphere microbial communities. In this study, three film-forming anti-desiccant products, TransFilm, Vapor Gard, and Wilt-Pruf were applied to the canopy of two boxwood cultivars ‘Vardar Valley’ and ‘Justin Brouwers’ on April 13 and August 26, 2021. Shoot samples were collected from boxwood plants treated with each of the three products, as well as nontreated control on June 16, August 26 (before the second treatment), and October 18. Microbial and plant genomic DNA was isolated together and 16S rRNA gene and the extended internal transcribed spacer regions were amplified with PCR and sequenced on a Nanopore MinION platform for bacterial and fungal identification. Results Bacterial communities were more diverse than fungal communities. At the phylum level, the boxwood phyllosphere was dominated by Proteobacteria and Ascomycota; at the genus level, Methylobacterium and Shiraia were the most abundant bacteria and fungi, respectively. Among the three film-forming anti-desiccants, Vapor Gard and Wilt-Pruf had more impact than TransFilm on the microbial communities. Specifically, broader impacts were observed on fungal than bacterial community composition and structure, with most affected fungi being suppressed while bacteria promoted. Conclusion This study addressed several major knowledge gaps regarding boxwood phyllosphere microbiota and the impact of anti-desiccants on plant microbiome. We identified diverse microbial communities of boxwood, a major evergreen woody crop and an iconic landscape plant. We also found differential effects of three film-forming anti-desiccants on the composition and structure of bacterial and fungal communities. These findings advanced our understanding of the associated microbiome of this landmark plant, enabling growers to fully utilize the potentials of microbiome and three anti-desiccants in improving boxwood health and productivity.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Boxwood phyllosphere fungal and bacterial communities and their differential responses to film-forming anti-desiccants

Li,

Omolehin,

Hemmings

et al. 2023

BMC Microbiol

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show abstract

“…Leaves provide a specific environment for microbial colonization, where the microbiota plays crucial roles in host performance and resilience to environmental perturbations [ 14 – 16 ]. For instance, there are many beneficial microbes in the plant phyllosphere, which can directly promote plant growth by improving nutrient acquisition or hormone stimulation, or indirectly affect plant health by inhibiting the growth of plant pathogens through competition and antagonism [ 16 – 18 ]. Moreover, phyllosphere microbes can degrade compounds harmful to plants, humans, or the environment, such as phenols [ 19 ], hydroquinones [ 20 ], and polyaromatic hydrocarbons [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Exploration of phyllosphere microbiomes in wheat varieties with differing aphid resistance

Li,

Wang,

Zhu

et al. 2023

Environmental Microbiome

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Background Leaf-associated microbes play an important role in plant development and response to exogenous stress. Insect herbivores are known to alter the phyllosphere microbiome. However, whether the host plant’s defense against insects is related to the phyllosphere microbiome remains mostly elusive. Here, we investigated bacterial communities in the phyllosphere and endosphere of eight wheat cultivars with differing aphid resistance, grown in the same farmland. Results The bacterial community in both the phyllosphere and endosphere showed significant differences among most wheat cultivars. The phyllosphere was connected to more complex and stable microbial networks than the endosphere in most wheat cultivars. Moreover, the genera Pantoea, Massilia, and Pseudomonas were found to play a major role in shaping the microbial community in the wheat phyllosphere. Additionally, wheat plants showed phenotype-specific associations with the genera Massilia and Pseudomonas. The abundance of the genus Exiguobacterium in the phyllosphere exhibited a significant negative correlation with the aphid hazard grade in the wheat plants. Conclusion Communities of leaf-associated microbes in wheat plants were mainly driven by the host genotype. Members of the genus Exiguobacterium may have adverse effects on wheat aphids. Our findings provide new clues supporting the development of aphid control strategies based on phyllosphere microbiome engineering.

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Enhanced control efficacy of Bacillus subtilis NM4 via integration of chlorothalonil on potato early blight caused by Alternaria solani

Noh,

Hwang,

Maung

et al. 2024

Microbial Pathogenesis

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Characterization of Boxwood Shoot Bacterial Communities and Potential Impact from Fungicide Treatments

Abstract: Agrochemicals are important tools for safeguarding plants from invasive pathogens, insects, mites, and weeds. How they may affect the plant microbiome, a critical component of crop health and production, was poorly understood.

Cited by 3 publications

References 91 publications

Boxwood phyllosphere fungal and bacterial communities and their differential responses to film-forming anti-desiccants

Boxwood phyllosphere fungal and bacterial communities and their differential responses to film-forming anti-desiccants

Exploration of phyllosphere microbiomes in wheat varieties with differing aphid resistance

Enhanced control efficacy of Bacillus subtilis NM4 via integration of chlorothalonil on potato early blight caused by Alternaria solani

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