Characterization of bovine viral diarrhea virus proteins

Purchio, Anthony F.; Larson, Ruby J.; Collett, Marc S.

doi:10.1128/jvi.50.2.666-669.1984

Cited by 71 publications

(35 citation statements)

References 13 publications

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“…While this protein apparently remains unchanged after infections with nonopBVDV, processing of p125 has been observed in cells infected with cpBVDV strains . A cleavage product of about 80 kDa (p80) has been described as a marker for the cytopathogenic viruses (Purchio et al ., 1984 ;Donis and Dubovi, 1 987a,b) . Using a monospecific antiserum the second processing product of 54 kDa (p54) could be detected in cells infected with BVDV NADL (Collett et al ., 1988b) .…”

Section: Proteins Expressed From the Recombinant Rnamentioning

confidence: 99%

Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences

Meyers¹,

Tautz²,

et al. 1991

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The RNA genomes of cytopathogenic bovine viral diarrhea virus (BVDV) isolates contain insertions highly homologous to cellular sequences. For two of them the insert was identified as ubiquitin coding sequence. The genome of BVDV Osloss contains exactly one ubiquitin gene monomer. In the case of BVDV CP1 the cellular insertion comprises one complete ubiquitin gene and part of a second monomer. The host cell-derived element in the CP1 genome is embedded in a large duplication of about 2.4 kb of viral sequences. Cellular insertion and duplication were not found in the genome of NCP1, the noncytopathogenic counterpart of CP1. These results strongly suggest that recombination between viral and cellular RNA is responsible for development of the cytopathogenic viruses, which is linked to pathogenesis of a lethal disease in cattle.

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Section: Proteins Expressed From the Recombinant Rnamentioning

confidence: 99%

Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences

Meyers¹,

Tautz²,

et al. 1991

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“…The 55K virion glycoprotein is presumably synthesized on membrane-bound polyribosomes. Our inability to detect virionspecific proteins programmed by membrane-bound polyribosomes could be due to the fact that our antisera, obtained from a BVDV-infected calf (10), may recognize only the native, properly glycolysated protein; proper glycolysation may not occur in vitro. Attempts to produce antibodies against the denatured 55K protein are in progress.…”

Section: Infection Of Bovine Kidney Cells With Bovine Viral Diarrheamentioning

confidence: 99%

“…results in the appearance of a single virusspecific RNA species of 8.2 kilobases (kb) (9). Three major virus-specific proteins have been detected in these cells by immunoprecipitation with antisera generated against BVDV in infected calves (10). These proteins have molecular weights of 115,000 (115K), 80K, and 55K.…”

Section: Infection Of Bovine Kidney Cells With Bovine Viral Diarrheamentioning

confidence: 99%

“…These proteins have molecular weights of 115,000 (115K), 80K, and 55K. The 55K protein was found to be glycosylated, and all three proteins were associated with BVDV virions (10). Since the only virusspecific RNA detected in infected cells is the 8.2-kb RNA species, it presumably must code for both structural and nonstructural proteins of the virus.…”

Section: Infection Of Bovine Kidney Cells With Bovine Viral Diarrheamentioning

confidence: 99%

“…Infection of bovine kidney cells with BVDV results in the synthesis of a single virus-specific nonpolyadenylated RNA species of 8.2 kb (9). This RNA is found in purified virions and presumably represents the genome of the virus (10). Infection of mosquito and BHK-21 cells with the flaviviruses Uganda S and West Nile virus also results in the synthesis of a single RNA species which is nonpolyadenylated (13).…”

Section: Infection Of Bovine Kidney Cells With Bovine Viral Diarrheamentioning

confidence: 99%

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Cell-free translation of bovine viral diarrhea virus RNA

Purchio¹,

Larson²,

Torborg³

et al. 1984

J Virol

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Bovine viral diarrhea virus RNA was translated in a reticulocyte cell-free protein synthesizing system. The purified, 8.2-kilobase, virus-specific RNA species was unable to serve as an efficient message unless it was denatured immediately before translation. In this case, several polypeptides, ranging in molecular weight from 50,000 to 150,000 and most of which were immunoprecipitated by bovine viral diarrhea virus-specific antiserum, were synthesized in vitro. When polyribosomes were used to program cell-free synthesis, mature viral 80,000and 115,000-molecular-weight proteins were detected; no precursor to the viral 55,000-molecularweight glycoprotein was noted. The implications of these results with respect to virus-specific protein synthesis are discussed.

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Genome Instability in BVDV: An Examination of the Sequence and Structural Influences on RNA Recombination

1998

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The cytopathogenic biotype of the pestivirus, bovine viral diarrhea virus, is frequently a product of nonhomologous recombination in the region of the genome encoding the viral NS2-NS3 proteins. The possibility that sequences or structures in this region contributed to a hotspot for RNA recombination was examined. A PCR-based strategy was used to examine viral genomic RNA isolated from tissue samples of cattle persistently infected with the noncytopathogenic biotype of the virus. Analysis of two different regions of the viral genome revealed that recombination was not restricted to particular sequences. Alignment of the genomic sequences undergoing recombination and examination of the predicted secondary structures of the participating RNAs revealed that the dissociation of partial, newly synthesized negative strand RNAs from the positive strand template occurred at many different sites on the molecule. Similarly, it appeared that the reassociation of the RNA polymerase complex with a second positive strand template was frequently influenced by short regions of homology between the nascent RNA strand and open secondary structures in the template molecule.

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Characterization of bovine viral diarrhea virus proteins

Cited by 71 publications

References 13 publications

Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences

Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences

Cell-free translation of bovine viral diarrhea virus RNA

Genome Instability in BVDV: An Examination of the Sequence and Structural Influences on RNA Recombination

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