Characterization of bone marrow stromal cells from multiple myeloma

Gregoretti, M. G.; Gottardi, Daniela; Bergui, Luciana; Merico, F.; Marchisio, Pier Carlo; Caligaris-Cappio, Federico

doi:10.1016/0145-2126(94)90067-1

Cited by 37 publications

(34 citation statements)

References 44 publications

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“…These cells communicate through a complex series of molecular pathways including adhesion molecules, cytokines, chemokines, growth factors and their respective receptors that may be critical for disease progression. Previous studies suggest that bone marrow stromal cells (BMSCs) in myeloma patients differ from those of healthy donors [1][2][3][4]8,19,20]. We reported elsewhere that BMSCs of multiple myeloma patients, in comparison to a control, produced more IL-6, IL-10, TNF-α, HGF, OPN and BAFF in response to RPMI8226 cells [21].…”

Section: Introductionmentioning

confidence: 87%

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

Zdzisińska

Roliński²,

Piersiak³

et al. 2009

Folia Histochem Cytobiol.

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Abstract:We examined cytokine production by bone marrow stromal cells (BMSCs) of patients with multiple myeloma (MM) in response to contact with myeloma RPMI8226 cells in standard 2-dimensional (2D) cultures and in 3-dimensional (3D) cultures on a gelatine sponge scaffold. It was detected that BMSCs in the 3D cultures produced more IL-11 and HGF and less IL-10 than in the 2D cultures. Moreover, RPMI8226 cells after contact with BMSCs in 3D cultures produced more sIL-6R than in the classic 2D cultures. We concluded that 3D cultures of BMSCs with myeloma cells offered a promising model for in vitro examination of interactions between myeloma cells and the bone marrow stroma and for examination of potent antimyeloma agents.

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Section: Introductionmentioning

confidence: 87%

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

Zdzisińska

Roliński²,

Piersiak³

et al. 2009

Folia Histochem Cytobiol.

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“…In addition, we found some increase in the proportions of CD45 low D7-FIB þ LNGFR þ cells in myeloma and MGUS. Interestingly, an increased stromal proliferation has been reported for myeloma in vitro (30), and this study provides a strategy for the investigation of MSCs in this disease prior to culture manipulation.…”

Section: Discussionmentioning

confidence: 99%

Optimization of a flow cytometry‐based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow

Jones

English

Kinsey

et al. 2006

Cytometry Part B Clinical

162

112

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“…[15][16][17][18] Previous studies suggested that the BMMSCs in MM differ from those of healthy donors. 19,20 In particular, MM BMMSCs expressed less CD106 and fibronectin, and 2.8 times more IL1b as compared with normal BMMSCs 21 and showed abnormal synthesis of hyaluronan. 22,23 Taken together, these data suggest that MM BMMSCs differ from their normal counterparts.…”

Section: Introductionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells are abnormal in multiple myeloma

et al. 2007

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Recent literature suggested that cells of the microenvironment of tumors could be abnormal as well. To address this hypothesis in multiple myeloma (MM), we studied bone marrow mesenchymal stem cells (BMMSCs), the only long-lived cells of the bone marrow microenvironment, by gene expression profiling and phenotypic and functional studies in three groups of individuals: patients with MM, patients with monoclonal gamopathy of undefined significance (MGUS) and healthy agematched subjects. Gene expression profile independently classified the BMMSCs of these individuals in a normal and in an MM group. MGUS BMMSCs were interspersed between these two groups. Among the 145 distinct genes differentially expressed in MM and normal BMMSCs, 46% may account for a tumor-microenvironment cross-talk. Known soluble factors implicated in MM pathophysiologic features (i.e. IL (interleukin)-6, DKK1) were revealed and new ones were found which are involved in angiogenesis, osteogenic differentiation or tumor growth. In particular, GDF15 was found to induce dosedependent growth of MOLP-6, a stromal cell-dependent myeloma cell line. Functionally, MM BMMSCs induced an overgrowth of MOLP-6, and their capacity to differentiate into an osteoblastic lineage was impaired. Thus, MM BMMSCs are abnormal and could create a very efficient niche to support the survival and proliferation of the myeloma cells.

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Characterization of bone marrow stromal cells from multiple myeloma

Cited by 37 publications

References 44 publications

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

A comparison of cytokine production in 2-dimensional and 3-dimensional cultures of bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

Optimization of a flow cytometry‐based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow

Bone marrow mesenchymal stem cells are abnormal in multiple myeloma

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