Characterization of bone‐marrow‐derived rat mesenchymal stem cells depending on donor age

Abstract

It is generally accepted that autologous transfers, as non-immunogenic, constitute the safest approach in cellular transplantations. However, this attitude is often associated with the need for isolation and extracorporeal propagation of cells derived from aged patients. Thus the knowledge about relationship between aging and the properties of MSCs (mesenchymal stem cells) is crucial in developing new clinical strategies. The aim of this study was to perform complex comparison of MSC derived from young and aged individuals, which included phenotype, proliferating rate, osteogenic and adipogenic potential and secretory activity. Evaluated populations were isolated from bone marrow of 3-month-old and 24-month-old rats. There was no significant difference in membrane antigen expression and PDT (population doubling time). Additionally, the adipogenic and osteogenic potential did not vary between studied populations. The reaction of MSCs to either mitogen [bFGF (basic fibroblas t growth factor)] or oxidative stress (H2O2) in vitro displayed a very similar pattern in both analysed populations. There was no difference in TGFβ1 (transforming growth factor β1) and VEGF (vascular endothelial growth factor) secretion measured by ELISA test and gene expression evaluated by real-time PCR. However, the expression of the gene for IL-1α (interleukin-1α) was 8-fold lower in oMSC (MSC isolated from old rats). These results indicate that aging individuals can be considered as candidates for autologous transplantation of bone-marrow-derived MSCs.

Isolation of a Stable Subpopulation of Mobilized Dental Pulp Stem Cells (MDPSCs) with High Proliferation, Migration, and Regeneration Potential Is Independent of Age

“…On the other hand, no changes were observed in MSCs with age has been reported in their osteogenic and adipogenic differentiation potential in vivo [15], [16] and in vitro [17], [18]. Furthermore, isolated CD105 + MSCs from the aged does not influence the adipogenic, myogenic [19], and osteogenic potential [20].…”

Effects of Aging on Osteogenic Response and Heterotopic Ossification Following Burn Injury in Mice

“…Sufficient evidence suggests that this traumainduced HO forms through an endochondral ossification process which begins with the condensation of MSCs [12,[30][31][32][33]. The reduction in osteogenic potential of MSCs from aged sources has already been reported [13,14]. However, the specific relationships between the inflammatory insult of a burn injury, age, and their respective contributions to MSC osteogenic differentiation have not been adequately explored.…”

“…With this paradigm of MSC involvement in mind, and the discrepancy between young and old patients seen clinically, questions arise about the effect of age on MSC differentiation. Recent studies have compared the in vitro differentiation capability of MSCs with varying donor age, but report some conflicting results [13,14]. Of particular interest regarding the role of MSCs in HO formation is their osteogenic response to global inflammatory insult.…”

The Effect of Endoscopic Administration of Autologous Porcine Muscle-derived Cells Into the Urethral Sphincter

“…Additionally, the porcine MDCs (pMDCs) were exposed to adipogenic media, as described previously. 18 Fibroblasts isolated from the skin of the same donors served as control. After 10 days of induction, the lipid droplets were visualized with Oil Red O staining (SigmaAldrich).…”