Characterization of BoLA-DRB3.2 Alleles in Hanwoo (Korean cattle) by Sequence Based Typing (SBT)

Abstract

A study was conducted with 70 Hanwoo (Korean cattle) for genotyping bovine leukocyte antigen (BoLA)-DRB3.2 gene by using the polymerase chain reaction (PCR) and sequence based typing (SBT). Two-step PCR was carried out for amplifying a 284 bp fragment of the target gene and the PCR products were digested with three restriction enzymes namely RsaI, BstYI and HaeIII. Seventeen alleles were detected with frequencies ranging from 1.43 to 18.57% and one (x'aa) of these alleles was identified as a new allele that has not been reported before. The frequency of the new x'aa allele identified in this breed was 12.86%. In addition, the seven most frequently observed alleles (DRB3.2 *10, *15, *16, *26, *27, *54 and x'aa) accounted for 74.28% of the alleles in this population. The phylogenetic tree showed that the BoLA-DRB3.2 allele sequences of Hanwoo were shared with other Bos taurus breeds and no specific clade for Hanwoo was identified. It indicates high heterogeneity of the BoLA-DRB3 gene in this population and may give some ideas for breeding animals having better disease resistance.

Genetic assessment of BoLA-DRB3 polymorphisms by comparing Bangladesh, Ethiopian, and Korean cattle

“…We observed a close genetic relationship between the Ethiopian cattle breeds and Asian breeds from the inferred phylogenetic tree and median joining-network tree. The close genetic relationship between Ethiopian cattle and Bangladesh Red Chittagong is consistent with the evidence of all Ethiopian cattle populations shared a higher proportion of Asian zebu ancestry [47][48][49].…”

The Diversity of BoLA-DRB3 Gene in Iranian Native Cattle

“…Many studies have indicated that differences exist between breeds of cattle and other animals with regard to frequencies of MHC class II alleles. In cattle, interpretations of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing studies indicate there are significant differences in allelic frequencies of BoLA-DRB3.2 in Jersey (Gilliespie et al, 1999), Holstein (Dietz et al, 1997), Argentine Creole (Gimovambattista et al, 2001, Japanese Shorthorn (Takeshima et al, 2002) and Brazilian dairy Gir cattle (da Mota et al, 2002), Black Pied (Sulimova et al, 1997), Ayrshire (Udina et al, 2003), Hanwoo (Jeong et al, 2007) and Iranian Holstein cattle (Nassiry et al, 2004). Thus, the frequencies of alleles of BoLA-DRB3 genes in different populations allow the differentiation and reconstruction of genetic distance among populations, providing a molecular basis for determination of the possible common origin of populations and can be used to reduce spreading of alleles providing susceptibility to disease in a cattle herd.…”