Characterization of blue green algae isolated from Egyptian rice field with potential anti-hepatitis C active components

Amer, Ranya; Wahab, Abeer Abdel; Fathy, Sahar M.F.; Salama, Osama; Demellawy, Maha A El

doi:10.5897/ajb2013.13177

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…The toxic bloom-forming species include the genera Microcystis , Nodularia , Aphanizomenon , Anabaena , Cylindrospermopsis , and Oscillaroria [ 23 , 26 – 28 ]. Their toxins are classified according to their mode of action in vertebrates as hepatotoxins, cytotoxins, neurotoxins, dermatotoxins, and irritants [ 24 , 27 , 29 ]. Among all cyanotoxins, microcystin and nodularin are the most common hepatotoxins produced by a wide range of blue-green algae [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Harmful Algal Blooms in Freshwater Sources of Mindu and Nyumba ya Mungu Dams, Tanzania

Gobry,

Bachwenkizi,

Kimambo

et al. 2023

Journal of Toxicology

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Harmful algal blooms (HABs) pose a significant threat to aquatic ecosystems and human health due to the production of toxins. The identification and quantification of these toxins are crucial for water quality management decisions. This study used DNA analysis (PCR techniques) to identify toxin-producing strains and liquid-chromatography-tandem mass spectrometry (LC-MS/MS) to quantify microcystins in samples from Mindu and Nyumba ya Mungu Dams in Tanzania. The results showed that HABs were detected in both dams. The BLAST results revealed that the 16S gene sequences of uncultured samples were very similar to an Antarctic cyanobacterium, Leptolyngbya sp, Anabaena sp, and Microcystis aeruginosa. Sequences of the cultured samples were most similar to Nodularia spumigena, Amazoninema brasiliense, Anabaena sp, and Microcystis aeruginosa. Further analyses showed that the nucleotide sequence similarity of uncultured isolates from this study and those from the GenBank ranged from 85 to 100%. For cultured isolates from this study and others from the GenBank, nucleotide identity ranged from 81 to 100%. The molecular identification of Microcystis aeruginosa confirmed the presence of HABs in both Mindu and Nyumba ya Mungu Dams in Tanzania. At Mindu Dam, the mean concentrations (± standard deviation) of microcystin-LR, -RR, and -YR were 1.08 ± 0.749 ppm, 0.120 ± 0.0211 ppm, and 1.37 ± 0.862 ppm, respectively. Similarly, at Nyumba ya Mungu Dam, the concentrations of microcystin-LR, -RR, and -YR were 1.07 ± 0.499 ppm, 0.124 ± 0.0224 ppm, and 0.961 ± 0.408 ppm, respectively. This paper represents the first application of PCR and LC-MS/MS to study microcystins in small freshwater reservoirs in Tanzania. This study confirms the presence of toxin-producing strains of Microcystis aeruginosa in both dams and also provides evidence of the occurrence of microcystins from these strains. These findings contribute in improving the monitoring of HABs contamination and their potential impact on water quality in Tanzanian reservoirs.

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Section: Introductionmentioning

confidence: 99%

Occurrence of Harmful Algal Blooms in Freshwater Sources of Mindu and Nyumba ya Mungu Dams, Tanzania

Gobry,

Bachwenkizi,

Kimambo

et al. 2023

Journal of Toxicology

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Ocean acidification alters the responses of invertebrates to wound-activated infochemicals produced by epiphytes of the seagrass Posidonia oceanica

Mutalipassi

Fink

Maibam

et al. 2020

Journal of Experimental Marine Biology and Ecology

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Seasonal Occurrence of Cyanobacteria and First Detection of Microcystin-LR in Water Column of Foum-Gleita Reservoir, Mauritania

Sadegh¹,

Sidoumou²,

Dia³

et al. 2021

Preprint

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This work was carried out to study the seasonal occurrence of cyanobacteria and their microcystin-LR in water column of Foum-Gleita reservoir (Mauritania). Limnological and biological factors were investigated at three depths (surface, -3, and -6 m) in this reservoir during a full year. Nutrients were analyzed by Spectrophotometry, phytoplankton was analyzed by Inverted Microscopy, microsystins were analyzed by High Performance Liquid Chromatography-tandem Mass Spectrometry and environmental factors relationships were analyzed by Pearson's correlation and Multiple Linear Regression. Physicochemical analyzes have shown that this reservoir is hypereutrophic with dissolved inorganic nitrogen and total phosphorus concentrations relatively high, varying from 1.39 to 6.53 and 0.21 to 0.57 mg/L, respectively. Annual surface water temperature was exceptionally high (27.8 ± 3.6°C), characterizing of a Sahelian climatic conditions. Phytoplankton analyzes have shown dominance of two toxic cyanobacteria species Microcystis aeruginosa and Dolichospermum flos-aquae during warm season (May-September). Microcystins analysis revealed presence of only most toxic variant, microcystin-LR. Microcystin-LR concentration in the surface water samples, during cyanobacterial blooms, was consistently high (5.638 µg/L), exceeding 5-times the World Health Organization drinking water limit (1 µg/L), however, it was much lower (0.83 µg / L) at depth (-6 m). Analysis of environmental factors relationships showed that the most influential factors on abundance of Microcystis aeruginosa and Dolichospermum flos-aquae and variability of microcystin-LR concentrations were total phosphorus, dissolved inorganic nitrogen, iron, temperature and pH. Finally, the study clearly demonstrated need for regular monitoring of cyanobacteria and cyanotoxins in the waters of studied reservoir.

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Characterization of blue green algae isolated from Egyptian rice field with potential anti-hepatitis C active components

Cited by 3 publications

References 38 publications

Occurrence of Harmful Algal Blooms in Freshwater Sources of Mindu and Nyumba ya Mungu Dams, Tanzania

Occurrence of Harmful Algal Blooms in Freshwater Sources of Mindu and Nyumba ya Mungu Dams, Tanzania

Ocean acidification alters the responses of invertebrates to wound-activated infochemicals produced by epiphytes of the seagrass Posidonia oceanica

Seasonal Occurrence of Cyanobacteria and First Detection of Microcystin-LR in Water Column of Foum-Gleita Reservoir, Mauritania

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