Characterization of blaOxA-23 gene regions in isolates of Acinetobacter baumannii

Wang, Dongguo; Yan, D.; Hou, Wei; Zeng, Xiaohua; Qi, Yue; Chen, Jiayu

doi:10.1016/j.jmii.2014.01.007

Cited by 18 publications

(9 citation statements)

References 35 publications

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“…While carbapenems have the most extended spectrum of antibacterial activity of all the beta‐lactams, the carbapenem resistance rate in A. baumannii has reached more than 50% worldwide in the past decade as reported in previous studies (Chagas et al ; Wang et al ; Ning et al ; Pfeifer et al ; Rieber et al ). Overall, CRAB isolates are resistant to multiple drugs which forcefully contribute to further outbreaks due to the limited treatment alternatives (Migliavacca et al ; Ning et al ; Rieber et al ).…”

Section: Discussionmentioning

confidence: 70%

Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

et al. 2019

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Aims: The present study was conducted to investigate the mechanism of carbapenem resistance and the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates collected from two nearby hospitals in Tehran, Iran. Methods and Results: A total of 180 CRAB isolates were studied. Antimicrobial susceptibility testing was performed using disk diffusion and Epsilometer tests. The detection of OXA-23, -24 and -58 was implemented for all isolates using polymerase chain reaction. Subsequently, isolates harbouring OXA-24 and -58 were investigated for the presence of resistance determinants of Ambler class A, metallo-b-lactamases (MBLs), and carbapenem-hydrolysing class D b-lactamases, ISAba1, and the genetic relatedness between them was analysed using pulsed-field gel electrophoresis (PFGE). All isolates were found to be resistant to imipenem with a MIC of ≥8 µg ml À1 and were susceptible to colistin with a MIC of ≤1Á5 µg ml À1 . Sixty percent of the isolates had OXA-23. OXA-24 and -58 were detected in 31 of 180 CRAB isolates. These chosen isolates were devoid of MBLs and bla SHV , bla CTX-M , bla VEB ESBL genes. The PER determinant was detected in 38% of isolates as the most common extended spectrum b-lactamases (ESBLs). Of these isolates, 51Á6% had OXA-23, and ISAba1 was found to be upstream of OXA-23 and OXA-51 in 16 and 8 isolates, respectively. The band patterns produced by PFGE showed nine clonal pulsotypes distributed between the two hospitals. Conclusion: The findings showed that the refractory CRAB isolates were transmitted intra-and inter-hospital, particularly in the ICU due to shortcomings in infection control surveillance. Significance and Impact of the Study: Carbapenem resistance is a substantial threat in the treatment of infections caused by A. baumannii due to limitations in the therapeutic options.

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Section: Discussionmentioning

confidence: 70%

Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

et al. 2019

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“…At the genus level, the presence of Acinetobacter , Aliivibrio , Marinomonas , Pseudoalteromonas , and Shewanella had positive associations with obesity ( Table 3 ). Acinetobacter is a genus of Gram-negative bacteria, and the species Acinetobacter baumannii is a key pathogen of infections in hospitals [ 48 ]. Aliivibrio is a reclassified genus from the “ Vibrio fischeri species group” [ 49 ], and species of Aliivibrio are symbiotic with marine animals or are described as fish pathogens [ 50 – 52 ].…”

Section: Discussionmentioning

confidence: 99%

Systematic Analysis of the Association between Gut Flora and Obesity through High-Throughput Sequencing and Bioinformatics Approaches

Chiu

Huang

Weng

et al. 2014

BioMed Research International

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Eighty-one stool samples from Taiwanese were collected for analysis of the association between the gut flora and obesity. The supervised analysis showed that the most, abundant genera of bacteria in normal samples (from people with a body mass index (BMI) ≤ 24) were Bacteroides (27.7%), Prevotella (19.4%), Escherichia (12%), Phascolarctobacterium (3.9%), and Eubacterium (3.5%). The most abundant genera of bacteria in case samples (with a BMI ≥ 27) were Bacteroides (29%), Prevotella (21%), Escherichia (7.4%), Megamonas (5.1%), and Phascolarctobacterium (3.8%). A principal coordinate analysis (PCoA) demonstrated that normal samples were clustered more compactly than case samples. An unsupervised analysis demonstrated that bacterial communities in the gut were clustered into two main groups: N-like and OB-like groups. Remarkably, most normal samples (78%) were clustered in the N-like group, and most case samples (81%) were clustered in the OB-like group (Fisher's P value = 1.61E − 07). The results showed that bacterial communities in the gut were highly associated with obesity. This is the first study in Taiwan to investigate the association between human gut flora and obesity, and the results provide new insights into the correlation of bacteria with the rising trend in obesity.

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“…Tn2007, which was identified in plasmids pAb-B004d-c_3 and pAb-D10a-a_3 is relatively uncommon with no plasmids listed on PLSDB and only one in GenBank (pAB14) carrying it. However, its detection has been reported in isolates from Algeria, France, Spain, China, and Tanzania (Corvec et al, 2007;Guerrero-Lozano et al, 2015;Wang et al, 2015;Kumburu et al, 2019). The identification of Tn2007 in samples from Ghana, within a plasmid that share a high level of sequence identity with pAB14 (identified in Algeria and Tanzania) strongly supports the importance of plasmids in the spread of carbapenemase genes, especially in Africa.…”

Section: Discussionmentioning

confidence: 81%

Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana

et al. 2020

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Characterization of blaOxA-23 gene regions in isolates of Acinetobacter baumannii

Abstract: Our results indicate that pattern A was the most prevalent molecular type based on PFGE, and that different clones might be widespread with a majority of ISAba1-bla(OxA-23) clonal lineages in the 15 PCR positive isolates of A. baumannii in the hospital.

Cited by 18 publications

References 35 publications

Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

Clonal relatedness and resistance characteristics of OXA‐24 and ‐58 producing carbapenem‐resistantAcinetobacter baumanniiisolates in Tehran, Iran

Systematic Analysis of the Association between Gut Flora and Obesity through High-Throughput Sequencing and Bioinformatics Approaches

Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana

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