Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging

Arbab, Ali S.; Bashaw, Lindsey A.; Miller, Bradley R.; Jordan, Elaine; Lewis, Bobbi K.; Kalish, Heather; Frank, Joseph A.

doi:10.1148/radiol.2293021215

Cited by 535 publications

(423 citation statements)

References 25 publications

(28 reference statements)

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“…Another labeling approach is based on combining a commercially available dextran-coated SPIO, such as Feridex s36 or Sinerem s , 37 and a commercially available transfection agent, for example, Superfectt poly-L-lysine, Lipofectamin or Fugenet. 35,38 Transfection agents effectively transport nanoparticles into cells through electrostatic interactions. However, each combination of transfection agent and dextran-coated SPIO nanoparticle has to be carefully titrated and optimized for different cell cultures, as lower concentrations of transfection agent may result in insufficient cellular uptake, whereas higher concentrations may induce the precipitation of complexes or may be toxic to the cells.…”

Section: Cell Labelingmentioning

confidence: 99%

Migration, fate and in vivo imaging of adult stem cells in the CNS

Syková

Jendelová

2007

Cell Death Differ

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Section: Cell Labelingmentioning

confidence: 99%

Migration, fate and in vivo imaging of adult stem cells in the CNS

Syková

Jendelová

2007

Cell Death Differ

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“…To account for effects of the admixed cytokines on host cells, we also included the corresponding "cytokine-alone" groups. To track the cells following implantation, hMAPCs were labeled with CFSE or iron particles (Resovist) 39,44 before injection. Irrespective of the cytokine cocktail used, localized areas of CFSE-labeled cells ( Figure 4A) and single Resovistlabeled hMAPC-derived cells ( Figure 4B) persisted for at least 10 days in the Matrigel plug as determined by in vivo live imaging and electron microscopy, respectively.…”

Section: Shh and Dll-4 Boost Arterial Ec Differentiation Of Hmapcs Anmentioning

confidence: 99%

In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells

Aranguren

Luttun

Clavel

et al. 2006

Blood

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IntroductionThe vascular system is a bipolar complex network of arteries that transport oxygen-rich blood to all tissues and veins that bring oxygendeprived blood back to the heart. 1 Because of this bipolar set-up, arteries and veins feature anatomic and physiological differences. Unlike venous endothelium, arterial endothelium is surrounded by several layers of smooth muscle cells (SMCs), separated by elastic laminae, and embedded in a thick layer of fibrillar collagen. 2 Moreover, both vessel types have a differential susceptibility to atherosclerotic disease, possibly due to exposure to different levels of shear stress. Arterial and venous endothelial cells (ECs) also have a distinct molecular signature, and such molecular specification occurs before the onset of blood flow. 3 Indeed, arteriovenous (AV) specification of ECs is accomplished early in development and is associated with the expression of a specific complement of factors: venous endothelium is characterized by the expression of EphB4, 4 Lefty-1, 5 Lefty-2, 5 COUP-TFII, 6 and MYO1-␤, 5 and arterial ECs express high levels of Notch 1 and 4, 7 Dll-4, 8 EphrinB1 and EphrinB2, 4 Jagged-1 and -2, 7 connexin-40, and Hey-2 (gridlock zebrafish ortholog). 9,10 Studies in Xenopus, zebrafish, and mice have revealed that, besides blood flow, 11 vessel-intrinsic cues and-later in development-signals from outside the vasculature 12,13 are implicated in defining arterial or venous fate such as members of the TGF-␤ pathway, 14,15 VEGF isoforms,13,[16][17][18]17 angiopoietins, 19 the Notch pathway, 7,9,20-22 the patched pathway, 20 and COUP-TFII, a member of the orphan nuclear receptor superfamily. 6 Although it has been shown that some of these pathways are well conserved from zebrafish to mouse, less information is available on whether they have a similar role in humans. Because these molecular differences between arterial and venous ECs exist independently of blood flow and some of these factors work in an EC-intrinsic way, 2 it should be possible to manipulate some or all of these to endow ECs with an arterial or venous fate. Consistent with this notion, recent studies have suggested that arterial markers can be induced in primary mature ECs. 5,13,21,23,24 Many different stem cell populations, including bone marrow (BM) mononuclear cells, AC133 ϩ endothelial progenitor cells, and embryonic stem cells have the potential to differentiate in vitro and in vivo into mature and functional ECs. 4,[25][26][27][28] We have recently described another stem cell population, multipotent adult progenitor cells (MAPCs), that differentiates into most somatic cell types, including functional ECs, in vitro and in vivo. [29][30][31][32][33] The question of whether and how these stem cells can be coaxed into arterial or venous ECs has thus far not been addressed. In this study, we analyzed the in vitro and in vivo arterial and venous endothelial differentiation of human MAPCs (hMAPCs) and hAC133 ϩ cells. Materials and methodsAdditional and extended descriptions of methods are inc...

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“…The nonefficient capture of SPION-DX has been reduced by coating these SPIONs with polymers (2) . Cationic compounds, such as Poly-L-Lysine (PLL) (8) have been used to help in the interaction between the SPION and the surface of the cells that present with negative charges, and consequently endosomal absorption (2,8) . PLL is a cationic polymer that presents with high density of positive changes in its chain, which leads to an attraction and consequent bond to molecules with predominantly negative charges (8) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine

Sibov

Miyaki

Mamani

et al. 2012

Einstein (São Paulo)

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OBJECTIVE: The objective of this study was to evaluate the effect of the labeling of umbilical cord vein derived mesenchymal stem cells with superparamagnetic iron oxide nanoparticles coated with dextran and complexed to a non-viral transfector agent transfector poly-L-lysine. METHODS: The labeling of mesenchymal stem cells was performed using the superparamagnetic iron oxide nanoparticles/dextran complexed and not complexed to poly-L-lysine. Superparamagnetic iron oxide nanoparticles/dextran was incubated with poly-L-lysine in an ultrasonic sonicator at 37°C for 10 minutes for complex formation superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine by electrostatic interaction. Then, the mesenchymal stem cells were incubated overnight with the complex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine and superparamagnetic iron oxide nanoparticles/dextran. After the incubation period the mesenchymal stem cells were evaluated by internalization of the complex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine and superparamagnetic iron oxide nanoparticles/dextran by Prussian Blue stain. Cellular viability of labeled mesenchymal stem cells was evaluated by cellular proliferation assay using 5,6-carboxy-fluorescein-succinimidyl ester method and apoptosis detection by Annexin V- Propidium Iodide assay. RESULTS: mesenchymal stem cells labeled with superparamagnetic iron oxide nanoparticles/dextran without poly-L-lysine not internalized efficiently the superparamagnetic iron oxide nanoparticles due to its low presence detected within cells. Mesenchymal stem cells labeled with the complex superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine efficiently internalized the superparamagnetic iron oxide nanoparticles due to greater presence in the cells interior. The viability and apoptosis assays demonstrated that the mesenchymal stem cells labeled and not labeled respectively with the superparamagnetic iron oxide nanoparticles/dextran/poly-L-lysine continue to proliferate over seven days and the percentage of cells in early or late apoptosis is low compared to the percentage of live cells over the three days. CONCLUSION: Our results showed that the use of poly-L-lysine complexed with superparamagnetic iron oxide nanoparticles/dextran provides better internalization of these superparamagnetic iron oxide nanoparticles in mesenchymal stem cells Thus, we demonstrated that this type of labeling is not cytotoxic to the mesenchymal stem cells, since the viability and apoptosis assays showed that the cells remain alive and proliferating. The efficiency of this type of labeling in mesenchymal stem cells can provide non-invasive methods for monitoring these cells in vivo.

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Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging

Abstract: Magnetic cellular labeling with the ferumoxides-PLL complex had no short- or long-term toxic effects on tumor or stem cells.

Cited by 535 publications

References 25 publications

Migration, fate and in vivo imaging of adult stem cells in the CNS

Migration, fate and in vivo imaging of adult stem cells in the CNS

In vitro and in vivo arterial differentiation of human multipotent adult progenitor cells

Evaluation of umbilical cord mesenchymal stem cell labeling with superparamagnetic iron oxide nanoparticles coated with dextran and complexed with Poly-L-lysine

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