Characterization of Biomphalaria orbignyi, Biomphalaria peregrina and Biomphalaria oligoza by polymerase chain reaction and restriction enzyme digestion of the internal transcribed spacer region of the RNA ribosomal gene

Spatz, Linus; Vidigal, Teofânia H. D. A.; Silva, Marcia C.A; Cappa, S. M. González; Carvalho, Omar S.

doi:10.1590/s0074-02762000000600010

Cited by 17 publications

(29 citation statements)

References 23 publications

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“…Some researcher used the same method (e.g. Oliveira et al, 2010) but others used the alternative polyacrylamide gel electrophoresis method (Abdel-Hamid et al, 2006;Oliveira et al, 2008;Spatz et al, 2000;Teixeira et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

Dajem¹,

Ibrahim²,

Al‐Quraishy³

et al. 2011

Gene

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Section: Discussionmentioning

confidence: 99%

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

Dajem¹,

Ibrahim²,

Al‐Quraishy³

et al. 2011

Gene

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“…In the Americas, schistosomiasis currently occurs in Brazil, Venezuela, Surinam, Puerto Rico, the Dominican Republic, and on several islands of the Lesser Antilles, with recent evidence indicating a spread from northeastern Brazil southward (Spatz et al, 2000; Kloos et al, 2008; Noya et al, 2015). An expansion of the current disease-distribution area can be expected, since the geographical range of snails that can act as intermedial hosts (IHs) is wider than that of the pathogen (Pan American Health Organization, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Paraense, 1966; Paraense, 1975a; Paraense, 2003; Rumi, 1991). Character similarity among the species, however, has in fact hampered classification, as the case of B. peregrina, B. orbignyi and B. oligoza found in Argentina whose morphological similarities difficult correct identification (Paraense, 1988; Spatz et al, 2000; Vidigal et al, 2000a; Estrada et al, 2006). Within the historical context, several of the South-American species were described in genera of doubtful taxonomic position, as the example of Taphius Adams & Adams, 1855; Biomphalaria Preston, 1910; Tropicorbis Brown & Pilsbry, 1914; Platytaphius Pilsbry, 1924; and Australorbis Pilsbry, 1934.…”

Section: Introductionmentioning

confidence: 99%

The South-American distribution and southernmost record of Biomphalaria peregrina—a potential intermediate host of schistosomiasis

Rumi

Vogler

Beltramino

2017

PeerJ

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Schistosomiasis remains a major parasitic disease, endemic in large parts of South America. Five neotropical species of Biomphalaria have been found to act as intermediate hosts of Schistosoma mansoni in natural populations, while others have been shown to be susceptible in experimental infections, although not found infected in the field. Among these potential intermediate hosts, Biomphalaria peregrina represents the most widespread species in South America, with confirmed occurrence records from Venezuela to northern Patagonia. In this study, we report the southernmost record for the species at the Pinturas River, in southern Patagonia, which finding implies a southward reassessment of the limit for the known species of this genus. The identities of the individuals from this population were confirmed through morphological examination, and by means of two mitochondrial genes, cytochrome oxidase subunit I (COI) and 16S-rRNA. With both markers, phylogenetic analyses were conducted in order to compare the genetic background of individuals from the Pinturas River with previously genetically characterized strains of B. peregrina from various South-American locations. In addition, we produced a potential distribution model of B. peregrina in South America and identified the environmental variables that best predict that distribution. The model was estimated through a maximum entropy algorithm and run with occurrence points obtained from several sources, including the scientific literature and international databases, along with climatic and hydrographic variables. Different phylogenetic analyses with either the COI or 16S-rRNA sequences did not conflict, but rather gave very similar topological organizations. Two major groups were identified, with sequences from the Pinturas River grouping together with haplotypes from subtropical and temperate regions. The model developed had a satisfactory performance for the study area. We observed that the areas with higher habitat suitability were found to be mainly linked to subtropical and temperate regions of South America between 15° and 45° south latitude, with different moderate- and low-suitability areas outside this range. We also identified the coldest temperatures as the main predictors of the potential distribution of this snail. Susceptibility surveys would be required to evaluate if southern populations of B. peregrina still retain their potential as intermediate hosts of S. mansoni.

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“…This species is morphologically very similar to B. peregrina, which is one of the most widespread planorbid species in the Neotropical region and a potential intermediate host of S. mansoni (Paraense 1966, 1975a, Paraense & Corrêa 1973. Recently, Spatz et al (2000), using PCR-RFLP, showed that it is possible to separate B. havanensis from B. peregrina and B. orbignyi.…”

Section: Discussionmentioning

confidence: 99%

Identification of Biomphalaria havanensis and Biomphalaria obstructa populations from Cuba using polymerase chain reaction and restriction fragment length polymorphism of the ribosomal RNA intergenic spacer

Vidigal

Caldeira

Simpson³

et al. 2001

Mem. Inst. Oswaldo Cruz

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Characterization of Biomphalaria orbignyi, Biomphalaria peregrina and Biomphalaria oligoza by polymerase chain reaction and restriction enzyme digestion of the internal transcribed spacer region of the RNA ribosomal gene

Abstract: The correct identification of

Cited by 17 publications

References 23 publications

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

The South-American distribution and southernmost record of Biomphalaria peregrina—a potential intermediate host of schistosomiasis

Identification of Biomphalaria havanensis and Biomphalaria obstructa populations from Cuba using polymerase chain reaction and restriction fragment length polymorphism of the ribosomal RNA intergenic spacer

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