Characterization of biofilm-forming cyanobacteria for biomass and lipid production

Bruno, Laura; Pippo, Francesca Di; Antonaroli, Simonetta; Gismondi, Alessandra; Valentini, C; Albertano, Patrizia

doi:10.1111/j.1365-2672.2012.05416.x

Cited by 60 publications

(31 citation statements)

References 43 publications

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“…Some pathogens may survive better in recycled water than in clean water [7–11]. Water quality may deteriorate, resulting in reduced availability of nutrient elements, underperformance of applied pesticides [12–14], and clogging of irrigation pipelines or emitters by microbial mats [15, 16]. …”

Section: Introductionmentioning

confidence: 99%

Diversity and community structure of cyanobacteria and other microbes in recycling irrigation reservoirs

2017

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Recycling irrigation reservoirs (RIRs) are emerging aquatic environments of global significance to crop production, water conservation and environmental sustainability. This study characterized the diversity and population structure of cyanobacteria and other detected microbes in water samples from eight RIRs and one adjacent runoff-free stream at three ornamental crop nurseries in eastern (VA1 and VA3) and central (VA2) Virginia after cloning and sequencing the 16S rRNA gene targeting cyanobacteria and chloroplast of eukaryotic phytoplankton. VA1 and VA2 utilize a multi-reservoir recycling irrigation system with runoff channeled to a sedimentation reservoir which then overflows into transition and retention reservoirs where water was pumped for irrigation. VA3 has a single sedimentation reservoir which was also used for irrigation. A total of 208 operational taxonomic units (OTU) were identified from clone libraries of the water samples. Among them, 53 OTUs (358 clones) were cyanobacteria comprising at least 12 genera dominated by Synechococcus species; 59 OTUs (387 clones) were eukaryotic phytoplankton including green algae and diatoms; and 96 were other bacteria (111 clones). Overall, cyanobacteria were dominant in sedimentation reservoirs, while eukaryotic phytoplankton and other bacteria were dominant in transition/retention reservoirs and the stream, respectively. These results are direct evidence demonstrating the negative impact of nutrient-rich horticultural runoff, if not contained, on natural water resources. They also help in understanding the dynamics of water quality in RIRs and have practical implications. Although both single- and multi-reservoir recycling irrigation systems reduce the environmental footprint of horticultural production, the former is expected to have more cyanobacterial blooming, and consequently water quality issues, than the latter. Thus, a multi-reservoir recycling irrigation system should be preferred where feasible.

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Section: Introductionmentioning

confidence: 99%

Diversity and community structure of cyanobacteria and other microbes in recycling irrigation reservoirs

2017

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“…Areal productivities of Tolypothrix sp. in the outdoor biofilm system from our study (0.8-1.2 g m −2 day −1 measured in single systems; simulated overall areal productivities of 0.6-1.1 g m −2 day −1 ) were in the range of low productivities of 0.02-3.3 g m −2 day −1 previously reported in biofilms of pure cyanobacterial cultures (Anabaena, Trichormus, Phormidium) (Bruno et al 2012) and mixed consortia of green algae and cyanobacteria (Synechococcus, Phormidium, Pseudanabaena) (Guzzon et al 2008) under controlled laboratory conditions (Table 2). No reliable data could be found in the literature regarding productivities of cyanobacterialdominated biofilm systems in outdoor conditions.…”

Section: Fig 5 Bioproduct Synthesis Bymentioning

confidence: 95%

“…Biofilm systems have been little studied for mass production of photosynthetic microorganisms aiming at bioproducts, with most focusing on green microalgae (e.g., Botryococcus braunii) (Ozkan et al 2012) and less frequently on cyanobacteria (Bruno et al 2012). However, there is extensive knowledge on the use of biofilm-based indoor and outdoor systems for remediation of a variety of waste waters (Kesaano and Sims 2014), with the "algal turf scrubber" being one of the most widely used systems.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, strains of certain N 2 -fixing cyanobacteria, e.g., Tolypothrix tenuis, are renowned for their self-flocculating ability ) that could potentially reduce dewatering costs in suspension culture. Additionally, as usual components of biofilms naturally present in waters and soils of all latitudes (Whitton 2012), cyanobacteria offer a source for exploring biofilm cultivation as an alternative to produce biomass with lower content of extracellular water than suspension cultures (Bruno et al 2012;Ozkan et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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First outdoor cultivation of the N2-fixing cyanobacterium Tolypothrix sp. in low-cost suspension and biofilm systems in tropical Australia

et al. 2015

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Tropical N 2 -fixing cyanobacteria offer an attractive alternative for production of biomass and bioproducts with potentially low cultivation and harvesting costs. The present study evaluated the biomass productivity of the N 2 -fixing cyanobacterium Tolypothrix sp. NQAIF319 grown in nitrogen-free medium in outdoor suspension and biofilm prototype cultivation systems in tropical Australia (Queensland). One-week cycles yielded maximum biomass productivitiesestimated based on ground area occupied by single systemsof 45-49 g dry weight m −2 day −1 (suspension) and 1.0-1.2 g dry weight m −2 day −1 (biofilm) with minimal biological contamination (Tolypothrix sp. biomass representing 94-98 % of the photosynthetic community). Moderate productivities of t h e pi g m e n t s ph y c o c y a n in/p hyc oe ryth rin ( 0.1 -2.8 g m −2 day −1 ), fatty acids (0.1-2.0 g m −2 day −1 ), and nitrogen stored in the biomass (0.1-5.9 g m −2 day −1 ) were reached in biofilm and suspension systems, respectively, opening avenues for production of low-value commodities with potentially big markets (nitrogen-rich biofertilizers and a q u a c u l t u r e f e e d ) a n d h i g h e r-v a l u e c h e m i c a l s (phycobiliproteins and fatty acids). Simulated multi-system arrangements yielded theoretical overall areal productivities four to six times lower than those in single systems thus highlighting the need for future tests fine-tuning inter-system separation to minimize shadowing while maximizing the efficiency in land use in larger-scale production plants. Biofilm and self-flocculated biomass showed 80-fold and 53-fold reduced extracellular-water contents compared to suspension cultures, respectively, which will need to be considered for techno-economic and water/carbon footprint evaluation of each of the possible bioproduct synthesis pathways. In conclusion, the flexible and simple prototypes developed together with the good properties of Tolypothrix sp. represent a promising platform for low-cost production of cyanobacterial bioproducts in tropical regions using low nitrogen-containing water sources.

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“…The conserved nature, variation in an orderly fashion across phylogenetic boundaries and lack of lateral gene transfer make 16S rDNA a suitable chronometer for taxonomic and phylogenetic studies. Widespread sequencing of ribosomal RNA genes (Bruno et al 2012;Ř eháková et al 2014;Keshari et al 2015Keshari et al , 2016Sciuto and Moro 2016) has added potentially significant information to the evolutionary phylogenetics of several cyanobacteria. Ribosomal RNA gene sequences or their polymorphisms are playing a pivotal role in studying molecular systematics and phylogenies of cyanobacteria by serving genetic markers for cyanobacterial species (Gordon and Giovannoni 1996).…”

Section: Introductionmentioning

confidence: 99%

A comparative study reveals the higher resolution of RAPD over ARDRA for analyzing diversity of Nostoc strains

Chakdar

Pabbi

2017

3 Biotech

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Nostoc is a diverse genus of filamentous cyanobacteria with tremendous potential for agricultural and industrial applications. Morphometric methods and routine 16S rDNA-based identification undermines the genetic diversity and impedes strain-level differentiation. A comparative study to deduce the discriminatory power of random amplified polymorphic DNA (RAPD) and amplified ribosomal DNA restriction analysis (ARDRA) for analyzing the genetic diversity of 20 Nostoc strains of diverse geographical origin was carried out. The RAPD primer used in the study generated 100% polymorphic profile. HIP TG primer produced the highest number of bands and fragments. Five primers, viz. OPA 08, OPA 11, HIP GC, OPAH 02 and OPF 05 could produce unique bands for 11 strains. Cluster analysis using the RAPD profile showed 12.5-25% similarity among the strains. Following in silico restriction analysis, two restriction enzymes, viz. HaeIII and HinfI were selected for ARDRA. However, clustering based on the restriction pattern showed 22.5-100% similarity. Results of the present study clearly indicate higher resolution of RAPD which can be reliably used for strain-level differentiation of Nostoc strains.

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Characterization of biofilm-forming cyanobacteria for biomass and lipid production

Cited by 60 publications

References 43 publications

Diversity and community structure of cyanobacteria and other microbes in recycling irrigation reservoirs

Diversity and community structure of cyanobacteria and other microbes in recycling irrigation reservoirs

First outdoor cultivation of the N2-fixing cyanobacterium Tolypothrix sp. in low-cost suspension and biofilm systems in tropical Australia

A comparative study reveals the higher resolution of RAPD over ARDRA for analyzing diversity of Nostoc strains

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