Characterization of bioactive compounds of Annona cherimola L. leaves using a combined approach based on HPLC-ESI-TOF-MS and NMR

Díaz‐de‐Cerio, Elixabet; Aguilera-Sáez, Luis Manuel; Gómez-Caravaca, Ana María; Verardo, Vito; Fernández‐Gutiérrez, Alberto; Fernández, Ignacio; Arráez‐Román, David

doi:10.1007/s00216-018-1051-5

Cited by 40 publications

(56 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other compounds in AELE remain unidentified and require further investigation. A study performed by Díaz-de-Cerio E. et al reports the presence of polar compounds in Annona cherimola leaves using a combined approach of MS and NMR techniques, as well as amino acids, carbohydrates, organic acids, phenolic acids and derivatives, cholines, flavonoids and phenylpropanoids [61].…”

Section: Discussionmentioning

confidence: 99%

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Ammoury

Younes

Khoury

et al. 2019

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundThe edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1).MethodsThe anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS).ResultsOur results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS.ConclusionAll the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.

show abstract

Section: Discussionmentioning

confidence: 99%

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Ammoury

Younes

Khoury

et al. 2019

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…Different bioactive compounds have been characterized in the plant, classified as sugars, amino acids, organic acids, carbohydrates, choline, phenolic acids and derivatives, flavonoids, phenylpropanoids, alkaloids, saponins, tannins, phenols, and phytosterols [27][28][29].…”

Section: Chemical Compositionmentioning

confidence: 99%

Emerging Exotic Fruits: New Functional Foods in the European Market

Cornara

Xiao

Smeriglio

et al. 2020

eFood

View full text Add to dashboard Cite

KeywordsSuperfruits functional food dragon fruit cherimoya finger lime star fruit A B S T R A C TThe consumption of exotic fruits is rapidly increasing in European countries. Some of these products have attracted much interest due to their alleged properties of preventing malnutrition, over-nutrition, and disease, maintaining a healthy body. Scientific studies on these fruits are multiplying, including chemical characterizations and biological investigations on in vitro and in vivo experimental models. This review concerns four edible fruits: Hylocereus undatus (dragon fruit), Annona cherimola (cherimoya), Citrus australasica (finger lime), and Averrhoa carambola (carambola or star fruit). By screening biomedical databases, viz. Scopus, WOS, and PubMed, a total of 131 papers have been selected. Data reveals a wide series of biological effects that confirm traditional medicinal uses or suggest new therapeutic applications. Most studies concern problems related to nutrition, such as body redox balance, metabolic syndrome, and hepatoprotective effects, but other properties have been highlighted, including anticancer, antimicrobial, anti-inflammatory, and neuroprotective effects, as well as cardiovascular and skin protection. Pharmacological investigations have also been focused on specific compounds, assuming a potential role in drug discovery. In summary, food products, byproducts, and single compounds derived from these plants could be exploited in the prevention of disease or for specific treatments of health problems.

show abstract

“…The RP C18 columns are the most used in various applications, including the study of bioactive compounds belonging to various chemical classes [26,27,28].…”

Section: Liquid Chromatography Modesmentioning

confidence: 99%

Liquid Chromatographic Strategies for Separation of Bioactive Compounds in Food Matrices

et al. 2018

View full text Add to dashboard Cite

Nowadays, there is an increasing attention for nutraceuticals and, in general, bioactive compounds naturally present in food. Indeed, the possibility of preserving human health and preventing disease (e.g., cardiovascular diseases, cancer etc.) by the intake of healthy food is attractive for both consumers and food industries. In turn, research in this field was also prompted significantly, with the aim of characterizing these bioactive compounds and ascribe to them a specific activity. The bioactive compounds can belong to several chemical classes. However, their chemical diversity and presence in complex matrices, such as food, make it challenging both their isolation and characterization. To tackle this issue, efficient separation systems are needed, which are mainly based on chromatography. In this context, this mini-review aims to provide the reader with an overview of the most relevant and recent approaches for the separation of the most common bioactive compounds in food, in particular polyphenols, phenols, carotenoids, and peptides, by liquid chromatography approaches.

show abstract

Characterization of bioactive compounds of Annona cherimola L. leaves using a combined approach based on HPLC-ESI-TOF-MS and NMR

Cited by 40 publications

References 39 publications

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Emerging Exotic Fruits: New Functional Foods in the European Market

Liquid Chromatographic Strategies for Separation of Bioactive Compounds in Food Matrices

Contact Info

Product

Resources

About