Characterization of biklf/klf17-deficient zebrafish in posterior lateral line neuromast and hatching gland development

Suzuki, Hiroaki; Ishizaka, Tomoe; Yanagi, Kanoko; Sone, Ryota; Sunaga, Yuto; Ohga, Rie; Kawahara, Atsuo

doi:10.1038/s41598-019-50149-5

Cited by 13 publications

(9 citation statements)

References 30 publications

(47 reference statements)

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“…In chicken, embryonic klf17 expression was detected in the primitive streak (44). Disruption of the klf17 gene in zebrafish prevents the embryos from hatching, resulting in lethality (45). Similar results were found in Xenopus embryos when klf17, also called Nepture, was blocked by antisense oligonucleotides (46).…”

Section: Discussionsupporting

confidence: 58%

Krüppel-like factor 17 upregulates uterine corin expression and promotes spiral artery remodeling in pregnancy

Wang

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Spiral artery remodeling is an important physiological process in the pregnant uterus which increases blood flow to the fetus. Impaired spiral artery remodeling contributes to preeclampsia, a major disease in pregnancy. Corin, a transmembrane serine protease, is up-regulated in the pregnant uterus to promote spiral artery remodeling. To date, the mechanism underlying uterine corin up-regulation remains unknown. Here we show that Krüppel-like factor (KLF) 17 is a key transcription factor for uterine corin expression in pregnancy. In cultured human uterine endometrial cells, KLF17 binds to the CORIN promoter and enhances the promoter activity. Disruption of the KLF17 gene in the endometrial cells abolishes CORIN expression. In mice, Klf17 is up-regulated in the pregnant uterus. Klf17 deficiency prevents uterine Corin expression in pregnancy. Moreover, Klf17-deficient mice have poorly remodeled uterine spiral arteries and develop gestational hypertension and proteinuria. Together, our results reveal an important function of KLF17 in regulating Corin expression and uterine physiology in pregnancy.

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Section: Discussionsupporting

confidence: 58%

Krüppel-like factor 17 upregulates uterine corin expression and promotes spiral artery remodeling in pregnancy

Wang

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…These cell types are linked through a large set of genes, including proteins involved in vesicular protein trafficking and several conserved transcription factors (TFs) such as myb , foxa1, xbp1 , and klf17 ( Figure 2D ), which all have documented functions in controlling the differentiation of secretory cell types ( Bennett et al, 2007 ; Dubaissi et al, 2014 ; Pan et al, 2014 ). For example, klf17 is expressed in zebrafish and frog hatching glands, and plays essential roles in regulating gland cell specification in both species ( Kurauchi et al, 2010 ; Suzuki et al, 2019 ). Together, the conserved cell type specification programs ( Erwin and Davidson, 2009 ) between developmentally distinct secretory cells support the notion that they may be transcriptionally and evolutionarily related despite having different developmental origins ( Arendt et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Mapping single-cell atlases throughout Metazoa unravels cell type evolution

Tarashansky

Musser

Khariton

et al. 2021

eLife

160

133

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Comparing single-cell transcriptomic atlases from diverse organisms can elucidate the origins of cellular diversity and assist the annotation of new cell atlases. Yet, comparison between distant relatives is hindered by complex gene histories and diversifications in expression programs. Previously, we introduced the self-assembling manifold (SAM) algorithm to robustly reconstruct manifolds from single-cell data (Tarashansky et al., 2019). Here, we build on SAM to map cell atlas manifolds across species. This new method, SAMap, identifies homologous cell types with shared expression programs across distant species within phyla, even in complex examples where homologous tissues emerge from distinct germ layers. SAMap also finds many genes with more similar expression to their paralogs than their orthologs, suggesting paralog substitution may be more common in evolution than previously appreciated. Lastly, comparing species across animal phyla, spanning mouse to sponge, reveals ancient contractile and stem cell families, which may have arisen early in animal evolution.

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“…Embryos were dehydrated in 80% ethanol and embedded using a Technovit kit (Kulzer) 30 . Embedded embryos were sectioned on a Leica RM2125 microtome at 7 μm and mounted on slides.…”

Section: Methodsmentioning

confidence: 99%

Critical roles of the ddx5 gene in zebrafish sex differentiation and oocyte maturation

Sone

Taimatsu

Ohga

et al. 2020

Sci Rep

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DEAD-box helicase 5 (Ddx5) functions as an ATP-dependent RNA helicase and as a transcriptional coactivator for several transcription factors; however, the developmental function of the ddx5 gene in vertebrates is not fully understood. We found that the zebrafish ddx5 gene was expressed in developing gonads. Using the genome editing technology transcription activator-like effector nuclease, we established a ddx5-disrupted zebrafish and examined the morphological phenotypes of the mutant. We found that the majority of ddx5-deficient mutants developed as fertile males with normal testes and a small number of ddx5-deficient mutants developed as infertile females with small ovaries. Apoptotic cell death at 31 days post fertilization was increased in thick immature gonads (presumptive developing ovaries) of the ddx5-deficient mutant compared to those of heterozygous wild-type fish, while the number of apoptotic cells in thin immature gonads (presumptive developing testes) was comparable between the mutant and wild-type animals. Histological analysis revealed that ovaries of adult ddx5-deficient females had fewer vitellogenic oocytes and a larger number of stage I and II oocytes. The amount of cyclic adenosine monophosphate in the ddx5-deficient ovaries was high compared to that of wild-type ovaries, presumably leading to the mitotic arrest of oocyte maturation. Therefore, the ddx5 gene is dispensable for testis development, but it is essential for female sex differentiation and oocyte maturation in zebrafish.

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Characterization of biklf/klf17-deficient zebrafish in posterior lateral line neuromast and hatching gland development

Cited by 13 publications

References 30 publications

Krüppel-like factor 17 upregulates uterine corin expression and promotes spiral artery remodeling in pregnancy

Krüppel-like factor 17 upregulates uterine corin expression and promotes spiral artery remodeling in pregnancy

Mapping single-cell atlases throughout Metazoa unravels cell type evolution

Critical roles of the ddx5 gene in zebrafish sex differentiation and oocyte maturation

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