Characterization of Beta-Lactam Resistance Genes and Virulence Factors Associated with Multidrug-Resistant Klebsiella pneumoniae Isolated from Patients at Major Hospitals in Trinidad, West Indies

Pustam, Aarti; Jayaraman, Jayaraj; Ramsubhag, Adash

doi:10.1007/s00284-022-02972-9

Cited by 2 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nine K . pneumoniae isolates were confirmed as previously predicted [ 32 ], while the isolate that was inaccurately characterized as K . pneumoniae using clinical laboratory methods was identified as K .…”

Section: Discussionsupporting

confidence: 75%

“…While the hypervirulent isolate from this study was obtained from a patient who was warded at the National Organ Transplant Unit in the country, and it was not surprising that the traits of hypervirulence were observed, the corroborating data on the specimen at the time of collection did not specify links to any specific disease. However, based on the basic background information on the host of this isolate [ 32 ] and the prevalence of several hvKp biomarkers in its virulome, we can speculate that this isolate may have been linked to invasive disease.…”

Section: Discussionmentioning

confidence: 99%

“…The ten clinical isolates used in this study are from a larger 2015–2017 study [ 32 ] and represent varying combinations of resistance genotypes and phenotypes as shown in S1 Table (Origin of local isolates). The isolates were selected randomly from genotype-phenotype profiles using the INDEX and Random functions in Microsoft ® Excel ® (Version 2301).…”

Section: Methodsmentioning

confidence: 99%

“…All the isolates were from clinical specimens including urine, sputum, and wound swabs, and originated from patients of 3 major hospitals in Trinidad, West Indies. Species identification, antibiotic resistance profiles, and virulence gene characterization of the isolates were previously reported [ 32 ]. While the fimH gene was present in most of the local isolates, there were differences in resistance phenotypes and genotypes of strains, e.g.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Comparative genomics and virulome analysis reveal unique features associated with clinical strains of Klebsiella pneumoniae and Klebsiella quasipneumoniae from Trinidad, West Indies

2023

Self Cite

View full text Add to dashboard Cite

Klebsiella pneumoniae and Klebsiella quasipneumoniae are closely related human pathogens of global concern. The more recently described K. quasipneumoniae shares similar morphological characteristics with K. pneumoniae and is commonly misidentified as this species using traditional laboratory techniques. The vast mobilome in these pathogenic bacteria influences the dissemination of virulence factors in high-risk environments and it is, therefore, critical to monitor strains for developing effective clinical management strategies. Herein, this study utilized Illumina sequencing to characterize the whole genomes of nine clinical K. pneumoniae and one K. quasipneumoniae isolate obtained from patients of 3 major hospitals in Trinidad, West Indies. Reconstruction of the assembled genomes and implementation of several bioinformatic tools revealed unique features such as high pathogenicity islands associated with the isolates. The K. pneumoniae isolates were categorized as classical (n = 3), uropathogenic (n = 5), or hypervirulent (n = 1) strains. In silico multilocus sequence typing, and phylogenetic analysis showed that isolates were related to several international high-risk genotypes, including sequence types ST11, ST15, ST86, and ST307. Analysis of the virulome and mobilome of these pathogens showed unique and clinically important features including the presence of genes associated with Type 1 and Type 3 fimbriae, the aerobactin and yersiniabactin siderophore systems, the K2 and O1/2, and the O3 and O5 serotypes. These genes were either on or in close proximity to insertion sequence elements, phage sequences, and plasmids. Several secretion systems including the Type VI system and relevant effector proteins were prevalent in the local isolates. This is the first comprehensive study investigating the genomes of clinical K. pneumoniae and K. quasipneumoniae isolates from Trinidad, West Indies. The data presented illustrate the diversity of Trinidadian clinical K. pneumoniae isolates as well as significant virulence biomarkers and mobile elements associated with these isolates. Additionally, the genomes of the local isolates will add to global databases and thus can be used in future surveillance or genomic studies in this country and the wider Caribbean region.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Comparative genomics and virulome analysis reveal unique features associated with clinical strains of Klebsiella pneumoniae and Klebsiella quasipneumoniae from Trinidad, West Indies

2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although, no post-operative complications due to this bacterium were observed, patient 7 remained colonized with one of the strains found pre-operatively. The presence of this bacterium in these patients suggests a community-acquired infection, not associated with the surgical or hospital procedures, which is usually the case for this microorganism [29]. This highlights the potential for the spread of multidrugresistant bacteria in the community, even in the absence of symptoms, which could lead to infection in susceptible hosts and pose a public health threat.…”

Section: Discussionmentioning

confidence: 79%

Detection of multidrug-resistant bacteria in the nasal cavities and evaluation of sinus disorders in patients undergoing Le Fort I osteotomy

Mendes,

Bortoli,

Zaleski

et al. 2024

BMC Oral Health

View full text Add to dashboard Cite

Introduction Orthognathic surgery can lead to sinus alterations, including sinusitis, attributed to the exposure of maxillary sinuses during Le Fort I osteotomy. Furthermore, being a hospital-based procedure, there is potential risk of complications arising from bacteria prevalent in such environments. This study evaluated maxillary sinusitis occurrence and the presence of multidrug-resistant bacteria in the nasal cavity before and after orthognathic surgery. Methods Ten patients with dentofacial deformities underwent Le Fort I osteotomy. Clinical evaluations using SNOT-22 questionnaire were performed, and nasal cavity samples were collected pre-surgery and 3–6 months post-surgery to quantify total mesophilic bacteria and detect Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae. Cone Beam Computed Tomography (CBCT) was performed pre- and post-operatively, and the results were evaluated using the Lund-Mackay system. This study was registered and approved by the Research Ethics Committee of PUCRS (No. 4.683.066). Results The evaluation of SNOT-22 revealed that five patients showed an improvement in symptoms, while two remained in the same range of interpretation. One patient developed post-operative maxillary sinusitis, which was not detected at the time of evaluation by SNOT-22 or CBCT. CBCT showed a worsening sinus condition in three patients, two of whom had a significant increase in total bacteria count in their nasal cavities. The Brodsky scale was used to assess hypertrophy in palatine tonsils, where 60% of the subjects had grade 1 tonsils, 20% had grade 2 and 20% had grade 3. None of the patients had grade 4 tonsils, which would indicate more than 75% obstruction. Two patients harboured S. aureus and K. pneumoniae in their nasal cavities. Notably, K. pneumoniae, which was multidrug-resistant, was present in the nasal cavity of patients even before surgery, but this did not result in maxillary sinusitis, likely due to the patients’ young and healthy condition. Conclusion There was an improvement in signs and symptoms of maxillary sinusitis and quality of life in most patients after orthognathic surgery. However, some patients may still harbour multidrug-resistant bacteria, even if they are asymptomatic. Therefore, a thorough pre-operative assessment is essential to avoid difficult-to-treat post-operative complications.

show abstract

Characterization of Beta-Lactam Resistance Genes and Virulence Factors Associated with Multidrug-Resistant Klebsiella pneumoniae Isolated from Patients at Major Hospitals in Trinidad, West Indies

Cited by 2 publications

References 44 publications

Comparative genomics and virulome analysis reveal unique features associated with clinical strains of Klebsiella pneumoniae and Klebsiella quasipneumoniae from Trinidad, West Indies

Comparative genomics and virulome analysis reveal unique features associated with clinical strains of Klebsiella pneumoniae and Klebsiella quasipneumoniae from Trinidad, West Indies

Detection of multidrug-resistant bacteria in the nasal cavities and evaluation of sinus disorders in patients undergoing Le Fort I osteotomy

Contact Info

Product

Resources

About