Characterization of Beauveria bassiana isolates from Japan using inter-simple-sequence-repeat-anchored polymerase chain reaction (ISSR-PCR) amplification

Abstract

DNA from 59 Beauveria bassiana isolates and one B. brongniartii isolate were subjected to inter-simple-sequence-repeat-anchored polymerase chain reaction (ISSR-PCR) amplification with three short, 14-18-nucleotide primers that included tandemly repeated sequences. ISSR-PCR was found to be highly reproducible since DNA amplification from several single-spore-isolate subcultures of the B. bassiana F-263 isolate resulted in identical banding patterns. Each primer produced highly polymorphic bands, and high gene diversities for each primer were estimated with a range from 0.24 to 0.28. Consequently, all B. bassiana isolates, including F-263, were characterized by unique banding patterns. A dendrogram created by unweighted pair group method analysis (UPGMA) of ISSR-PCR data showed several small clades composed of isolates that originated from the insect order Coleoptera. They were dispersed throughout the dendrogram and did not comprise a large clade. A similar pattern was found for isolates from Lepidoptera and Hymenoptera.

Investigation of the internal bacterial flora of Eurygaster integriceps (Hemiptera: Scutelleridae) and pathogenicity of the flora members

“…The reason for this might be that we didn't use local isolates against E. integriceps. Because, it is known that local isolates could be more suitable against the target pest in terms of ecological compatibility and negative effects on non-target organisms (Gulsar Banu et al 2004;Takatsuka 2007). Therefore, it should be more advantageous to isolate local isolates where the sunn pest lives and to test these isolates against it.…”

Molecular tracing of white muscardine in Asian corn borer using inter-simple sequence repeat (ISSR) analysis

“…bassiana populations were reported to be genetically diverse (Takatsuka, 2007;Fernandes et al, 2009), and host specialization varied among different isolates (Vestergaard et al, 2003). Molecular ecological approaches provide a precise and credible way to investigate genetic diversity and the population genetic structure of B. bassiana.…”

“…In comparison to other DNA molecular marker methods, ISSR was more easily used and reliable for assessing genetic diversity. In addition, ISSR has been used in previous studies of plants, animals, and fungi (Gupta et al, 1994;Zietkiewicz et al, 1994;Kerrigan et al, 2003;Wang et al, 2005;Takatsuka, 2007;Lihme et al, 2009). ISSR analysis of hundreds of isolates from a silkworm-producing region, allowed Li et al (2010Li et al ( , 2011 to confirm that silkworm diseases are genetically distant from those of pine moths.…”

Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers

“…acridum Driver, Milner & Trueman (Entz et al, 2005). The ISSR technique was used in studying the genetic variability of two varieties of M. anisopliae from different localities (Lima, 2005) and Beauveria bassiana (Balsamo) Vuillemin in China (Wang et al, 2005), Asia (Aquino de Muro et al, 2005) and Japan (Takatsuka, 2007). Group I introns, found in rDNA genes of eukaryotes, usually have irregular distribution, being present in some isolates and absent in others, which can become a source of genetic variability (Mavridou et al, 2000).…”