Characterization of Beauveria bassiana and Metarhizium anisopliae isolates for management of tarnished plant bug, Lygus lineolaris (Hemiptera: Miridae)

Liu, Houping; Skinner, Margaret; Brownbridge, Michael; Parker, Bruce L.

doi:10.1016/s0022-2011(03)00018-1

Cited by 84 publications

(67 citation statements)

References 43 publications

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“…The time taken for the germination of 50% conidia was estimated with a procedure slightly modified from Liu et al (2003) and Bugeme et al (2008). The rate of conidial germination of each isolate was assessed by spreading 20 μl of a 10 6 /ml conidial suspension on film of PDAY medium on a glass slide which in turn was kept in polystyrene Petri dishes lined with moist filter paper.…”

Section: Methodsmentioning

confidence: 99%

Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance

Teja

Rahman

2016

Mycology

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Entomopathogenic fungal species from the genera Beauveria, Metarhizium and Lecanicillium are important components in biological control of insect pests. However, temperature, humidity and UV radiation are among the important abiotic factors, which limit their effective usage. In this study, four local isolates of Metarhizium were isolated from different crop rhiospheres of Telangana and Andhra Pradesh states of India, identified and tested for their temperature tolerance in terms of radial growth, conidial yield per 10 mm disc and rate of conidial germination at different incubation temperatures. The results revealed that strains LaMa1 and MaAICRP performed well in terms of radial growth, conidial yield and rate of conidial germination, even at 35°C temperature. The role of such temperature tolerant strains in agriculture is discussed.

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Section: Methodsmentioning

confidence: 99%

Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance

Teja

Rahman

2016

Mycology

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“…It is currently recognized that B. bassiana is a species complex with several cryptic species, and that it is phylogentically affiliated to the Ascomycotina (the Clavicipitaceae family of Hypocreales of the Pyrenomycetes) [4,5]. The characterization of B. bassiana was based on the description and measurement of structures of agronomic interest [6], on its physiological [7] and pathogenic characteristics, [8] and on the results of isozymatic studies [9]. The characterization of B. bassiana was carried out using different kinds of DNA markers [10][11][12], RFLPs and RAPDs [13,14], ITSs [15], microsatellites (SSRs) and minisatellites [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

Estrada¹,

Camacho

Benito

2007

Cellular and Molecular Biology Letters

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Inter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.

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“…In a different study, the fungal strains with a spore length >3 μm and ratio of spores length to width ≥ 2have been reported to be B. brongniartii or one of the other Beauveria species [34]. Significant differences in spore size, production, speed of germination and relative hyphal growth among the different B. bassiana isolates was also reported by Liu et al [30] There have been few studies evaluating the efficacy of ENPFs for M. domestica control. In this regard, present study showing appreciable pathogenic activity of all 5 native B. bassiana isolates against adultand larvae life stages of M. domestica was commendable.…”

Section: Discussionmentioning

confidence: 94%

“…Similarly, Varela and Morales [29] reported wide variation (between white and yellow) in colony color of different B. bassiana isolates. Liu et al [30], reported variation in spore production (1.6 × 10 6 -1.56 × 10 7 spores/cm 2 ) for the four isolates of B. bassiana, with the most virulent isolate producing significantly larger number of spores. The value of hydrophobicity index denotes interfacial free energy for interaction [31].…”

Section: Discussionmentioning

confidence: 99%

Morpho-Molecular Characterization and Virulence Determination of Entomopathogenic Fungal Isolates Native to Indian Subcontinent

Mishra¹

2015

Mycology

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Background: The house fly, Musca domestica L. is a major pest of medical and veterinary significance which due to their ubiquitous lifestyle and broad food preference achieves a very high density in favorable climatic conditions of Indian subcontinent. This has led to several instances of severe health condition among human and animal population, calling attention towards M. domestica control through existing and innovative methods. Use of biological control agent, such as entomopathogenic fungi (ENPF) for M. domestica control has shown potential, and need to be further explored for the newer isolates suited to particular environment for greater efficacy. The present study attempted the isolation and identification of native ENPF strains suitable for M. domestica control.

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Characterization of Beauveria bassiana and Metarhizium anisopliae isolates for management of tarnished plant bug, Lygus lineolaris (Hemiptera: Miridae)

Cited by 84 publications

References 43 publications

Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance

Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance

The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

Morpho-Molecular Characterization and Virulence Determination of Entomopathogenic Fungal Isolates Native to Indian Subcontinent

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