Characterization of baculovirus Autographa californica multiple nuclear polyhedrosis virus infection in mammalian cells

Kitajima, Masayuki; Hamazaki, Hiroyuki; Miyano-Kurosaki, Naoko; Takaku, Hiroshi

doi:10.1016/j.bbrc.2006.02.167

Cited by 23 publications

(14 citation statements)

References 37 publications

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“…Also, transient disruption of intercellular junctions (Bilello et al, 2001) and transient disintegration of microtubule (Salminen et al, 2005) can respectively improve the entry and nuclear transport of baculovirus, and hence the expression. By comparing the transduction of permissive (Huh-7 and B16) and nonpermissive (Raw264.7 and YAC-1) cell lines using baculovirus-expressing EGFP under CMV promoter, Kitajima et al (2006) also found that baculovirus can enter all four cell lines with high efficiency, but the nuclear transport efficiency is considerably higher in the permissive Huh-7 cells and lower in the nonpermissive RAW264.7 and YAC-1 cells. All these reports collectively attest the importance of virus uptake and intracellular trafficking in baculovirus transduction.…”

Section: Discussionmentioning

confidence: 97%

Variation of baculovirus‐harbored transgene transcription among mesenchymal stem cell‐derived progenitors leads to varied expression

Lee

Hwang

et al. 2006

Biotech & Bioengineering

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We have previously demonstrated that baculovirus can efficiently transduce human mesenchymal stem cells (MSCs) and MSCs-derived adipogenic, chondrogenic, and osteogenic progenitors without compromising the differentiation capacity. Remarkably, the transgene expression level and duration varied widely with the differentiation states at which the progenitors were transduced. However, whether the variation was a general phenomenon and what caused the variation were unclear. Here we demonstrated that transduction of the MSCs and MSC-derived progenitors using baculoviruses carrying egfp driven by CMV, EF-1alpha or CAG promoter resulted in a general trend of varied expression, that is, the chondrogenic progenitors allowed for the poorest expression while the adipogenic progenitors conferred the best expression. Quantification of the nuclear and cytoplasmic egfp gene copy numbers by quantitative real-time PCR revealed that the varied expression did not arise from the discrepancies in gene delivery efficiency nor was it due to the disparities in nuclear transport efficiency. In contrast, the transcription levels paralleled the overall expression levels. These data suggested that although the egfp genes could be efficiently delivered into the nuclei of chondrogenic progenitors, they were not transcribed as well as they were in the adipogenic progenitors. In conclusion, the rapidly altering cellular transcription machinery in the course of differentiation progression predominantly led to the varied expression levels.

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Section: Discussionmentioning

confidence: 97%

Variation of baculovirus‐harbored transgene transcription among mesenchymal stem cell‐derived progenitors leads to varied expression

Lee

Hwang

et al. 2006

Biotech & Bioengineering

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“…The ability of AcMNPV to infect insect cells has led to its use in multiple protein expression systems (5,16) and as plant insecticides (8,21). AcMNPV also infects a variety of mammalian cell types, with the exception of certain hematopoietic cell lines, although its genome does not replicate or integrate into mammalian chromosomes (9,11,13,17,23).…”

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confidence: 99%

Induction of Antitumor Acquired Immunity by Baculovirus Autographa californica Multiple Nuclear Polyhedrosis Virus Infection in Mice

Kitajima

Takaku

2008

Clin Vaccine Immunol

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“…It should be considered that the analysis shown in Figure 1 allows the identification of the population expressing a recombinant protein (either GFPVP2 or VP6), but not necessarily that of infected cells, as expression of the recombinant gene might be absent or inefficient even in infected cells. A marker that has been used to monitor infection by baculovirus is gp64 [22]. gp64 is the major envelope glycoprotein of baculovirus [23], and accumulates in the membrane of infected cells during the first 10 to 12 hpi [24].…”

Section: Resultsmentioning

confidence: 99%

Population kinetics during simultaneous infection of insect cells with two different recombinant baculoviruses for the production of rotavirus-like particles

2007

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Background: The simultaneous production of various recombinant proteins in every cell of a culture is often needed for the production of virus-like particles (VLP) or vectors for gene therapy. A common approach for such a purpose is the coinfection of insect cell cultures with different recombinant baculoviruses, each containing one or more recombinant genes. However, scarce information exists regarding kinetics during multiple infections, and to our knowledge, no studies are available on the behavior of the different populations that arise during coinfections. Such information is useful for designing infection strategies that maximize VLP or vector yield. In this work, kinetics of cell populations expressing rotavirus GFPVP2 (infected with bacGFPVP2), VP6 (infected with bacVP6), or both proteins simultaneously (coinfected with both baculoviruses) were followed by flow cytometry.

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Characterization of baculovirus Autographa californica multiple nuclear polyhedrosis virus infection in mammalian cells

Cited by 23 publications

References 37 publications

Variation of baculovirus‐harbored transgene transcription among mesenchymal stem cell‐derived progenitors leads to varied expression

Variation of baculovirus‐harbored transgene transcription among mesenchymal stem cell‐derived progenitors leads to varied expression

Induction of Antitumor Acquired Immunity by Baculovirus Autographa californica Multiple Nuclear Polyhedrosis Virus Infection in Mice

Population kinetics during simultaneous infection of insect cells with two different recombinant baculoviruses for the production of rotavirus-like particles

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