Characterization of bacterial symbionts in Frankliniella occidentalis (Pergande), Western flower thrips

Chanbusarakum, Lisa; Ullman, Diane E.

doi:10.1016/j.jip.2008.09.001

Cited by 36 publications

(49 citation statements)

References 47 publications

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“…Previous observations of BFo-1 and BFo-2 in culture and plates showed that BFo-1 was more abundant than BFo-2 at room temperature (Chanbusarakum and Ullman 2008). This analysis conÞrmed that BFo-1 is more abundant within overnight cultures; however, the two bacterial types actually have similar growth rates.…”

Section: August 2009supporting

confidence: 52%

“…The prevalence of these speciÞc bacteria with wild F. occidentalis regardless of plant host, collection time, environmental characteristics, or host attributes conÞrms laboratorybased indications that this relationship is stable and widespread, Þtting the traditional deÞnition of a symbiosis (Sapp 2004) and supporting the contention that F. occidentalis have a symbiotic relationship with BFo-1 and BFo-2 (Chanbusarakum and Ullman 2008).…”

Section: Discussionmentioning

confidence: 54%

“…1), with two or more infection patterns present in 22 of the 30 sites. This prevalence differed noticeably from Hawaiian Islands laboratory colony infection rates, where cloning showed 30% were dually infected, 60% had exclusively BFo-1, 10% had BFo-2 only, and no thrips were without bacteria (Chanbusarakum and Ullman 2008). The Fisher exact test showed a signiÞcant association existed between the presence of BFo-1 and BFo-2 (P ϭ 0.001); speciÞcally, more thrips possessed both bacteria compared with being uninfected or having only one of the bacterial types.…”

Section: Prevalence and Distribution Of Bfo-1 And Bfo-2 Inmentioning

confidence: 55%

“…DNA from one or groups of 10 F. occidentalis adults from a single collection site were directly extracted using the Qiagen DNeasy kit. The bacterial 16S rDNA was ampliÞed and cloned following previously described methods (Chanbusarakum and Ullman 2008). PCR products were puriÞed using the Qiagen QIAquick PCR puriÞcation kit before being sent to the Division of Biological Sciences DNA Sequencing Facility (College of Biological Sciences, UC-Davis, Davis, CA) for sequencing with the primers 27f, 514r, 530f, and 860r to obtain approximately the Þrst 1,000 bp of the 16S rDNA ( Table 2).…”

Section: Methodsmentioning

confidence: 99%

“…However, numerous insectÐ bacteria interactions are not well understood, even for those bacteria that can be examined independently from their insect host (Harada et al 1997, Watanabe andSato 1998). One such relationship involves the Western ßower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), and their gut bacteria (de Vries et al 2001a(de Vries et al , b, 2004Chanbusarakum and Ullman 2008). Understanding how the bacteria interact with the thrips could lead to innovative strategies for management, a goal that is important because F. occidentalis are serious and largely unmanageable pests of numerous food, Þber, and ornamental crops (Boonham et al 2002).…”

mentioning

confidence: 99%

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Distribution and Ecology ofFrankliniella occidentalis(Thysanoptera: Thripidae) Bacterial Symbionts

Chanbusarakum

Ullman

2009

Environ Entomol

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Bacterial populations in Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) collected in diverse California environments consisted of two bacterial symbionts: BFo-1 and BFo-2 (B = bacteria, Fo = Frankliniella occidentalis, numbers reflect different types). Dual infections of BFo-1 and BFo-2 were found in 50% of the thrips, 18% had neither bacterium, and 24 and 8% were infected solely with BFo-1 and BFo-2, respectively. No other bacteria consistently infected F. occidentalis. Dual infections occurred more often in male thrips and in thrips of both sexes from southern mountain and valley sites. As average collection year or month minimum temperature decreased, infections of BFo-1, alone or in dual infections, increased significantly. As yearly precipitation increased, infection with BFo-1 alone also increased. F. occidentalis color morphology did not affect bacterial infection. BFo-1 created weak biofilms at 25 and 32 degrees C; BFo-2 made strong biofilms at 25 degrees C and no biofilms at 32 degrees C. When the bacteria were grown in culture together, weak biofilms formed at both temperatures studied, although there was no way to determine what each bacterium contributed to the biofilm. BFo-1 and BFo-2 grew at similar rates at 25 and 30 degrees C. Our data show BFo-1 and BFo-2 occur in natural populations of F. occidentalis and support the hypothesis BFo have a symbiotic relationship with F. occidentalis. Regional differences in bacterial prevalence suggest bacterial infection is associated with environmental conditions, and altitude, temperature, and precipitation may be important factors.

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Section: August 2009supporting

confidence: 52%

Section: Discussionmentioning

confidence: 54%

Section: Prevalence and Distribution Of Bfo-1 And Bfo-2 Inmentioning

confidence: 55%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Distribution and Ecology ofFrankliniella occidentalis(Thysanoptera: Thripidae) Bacterial Symbionts

Chanbusarakum

Ullman

2009

Environ Entomol

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The Biological Fight Against Pathogenic Bacteria and Protozoa

Villa

Sánchez-Pérez

Viñas

2016

New Weapons to Control Bacterial Growth

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Accessing the Hidden Microbial Diversity of Aphids: an Illustration of How Culture-Dependent Methods Can Be Used to Decipher the Insect Microbiota

Grigorescu

Renoz

Sabri³

et al. 2017

Microb Ecol

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Microorganism communities that live inside insects can play critical roles in host development, nutrition, immunity, physiology, and behavior. Over the past decade, high-throughput sequencing reveals the extraordinary microbial diversity associated with various insect species and provides information independent of our ability to culture these microbes. However, their cultivation in the laboratory remains crucial for a deep understanding of their physiology and the roles they play in host insects. Aphids are insects that received specific attention because of their ability to form symbiotic associations with a wide range of endosymbionts that are considered as the core microbiome of these sap-feeding insects. But, if the functional diversity of obligate and facultative endosymbionts has been extensively studied in aphids, the diversity of gut symbionts and other associated microorganisms received limited consideration. Herein, we present a culture-dependent method that allowed us to successfully isolate microorganisms from several aphid species. The isolated microorganisms were assigned to 24 bacterial genera from the Actinobacteria, Firmicutes, and Proteobacteria phyla and three fungal genera from the Ascomycota and Basidiomycota phyla. In our study, we succeeded in isolating already described bacteria found associated to aphids (e.g., the facultative symbiont Serratia symbiotica), as well as microorganisms that have never been described in aphids before. By unraveling a microbial community that so far has been ignored, our study expands our current knowledge on the microbial diversity associated with aphids and illustrates how fast and simple culture-dependent approaches can be applied to insects in order to capture their diverse microbiota members.

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Characterization of bacterial symbionts in Frankliniella occidentalis (Pergande), Western flower thrips

Cited by 36 publications

References 47 publications

Distribution and Ecology ofFrankliniella occidentalis(Thysanoptera: Thripidae) Bacterial Symbionts

Distribution and Ecology ofFrankliniella occidentalis(Thysanoptera: Thripidae) Bacterial Symbionts

The Biological Fight Against Pathogenic Bacteria and Protozoa

Accessing the Hidden Microbial Diversity of Aphids: an Illustration of How Culture-Dependent Methods Can Be Used to Decipher the Insect Microbiota

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