Characterization of Bacterial Community Diversity in Chronic Rhinosinusitis Infections Using Novel Culture-independent Techniques

Abstract

This study has validated the use of the culture-independent technique T-RFLP in sinonasal samples. Preliminary characterization of the microbial diversity in CRS suggests a complex range of common and novel bacterial species within the upper airway in CRS, providing further evidence for the polymicrobial etiology of CRS.

Chronic rhinosinusitis: a microbiome in dysbiosis and the search for alternative treatment options

“…Of particular importance to CRS is S. aureus. Most microbiome studies found that S. aureus was enriched in CRS patients; some even found it to be the most abundant organism in CRS sinuses 9,11,12,16,18 . Here it is important Figure 1.…”

“…16 , Corynebacterium spp. 10,16 , certain Streptococcus spp., Staphylococcus aureus, Propionibacterium acnes and Haemophilus influenzae 15,16 have been reported in CRS.…”

“…16 , Corynebacterium spp. 10,16 , certain Streptococcus spp., Staphylococcus aureus, Propionibacterium acnes and Haemophilus influenzae 15,16 have been reported in CRS. Abreu et al 10 notably found enrichment of a novel sinopathogen Corynebacterium tuberculostearicum, typically a commensal when present on skin.…”

“…Further adding to the complexity of CRS is that there does not seem to be a 'model' CRS microbiome; that is, each CRS patient has a unique sinus microbiome composition 15,16 . Also, even within a single CRS patient, the microbiome of each sinus is different 19 .…”

“…Traditional culture-dependent methods fail to truly represent the sinus microbiome 9,15 , so truly accurate insights into CRS versus healthy microbiomes are from a currently limited number of molecular-based studies [9][10][11][12][15][16][17][18] .…”