Characterization of bacterial communities in heavy metal contaminated soils

Roane, Timberley M.; Kellogg, S. T.

doi:10.1139/m96-080

Cited by 174 publications

(107 citation statements)

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“…Chromosome and plasmid encoded metal resistance genetic systems have been studied in Pseudomonas, as well as in related bacteria (Cervantes, 1996). Pseudomonas species resistant to cadmium up to 1.2 mM maximum tolerable concentration was isolated from heavy metal contaminated soil (Roane and Kellogg, 1996). P. aeruginosa BC15 resistant to cadmium up to 6 mM in LB agar plates (data not shown).…”

Section: Resultsmentioning

confidence: 97%

Plasmid profile and curing analysis of Pseudomonas aeruginosa as metal resistant

Raja

Selvam

2009

Int. J. Environ. Sci. Technol.

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AB STRACT:The isolate Pseudomonas aeruginosa exhibited resistance to heavy metals such as cadmium, chromium, nickel and lead. Plasmid DNA was isolated from P. aeruginosa and designated as pBC15. The size of the plasmid DNA was approximately 23 kb. Escherichia coli DH5α was transformed with plasmid pBC15 subsequired resistance to nickel and ampicillin. The same size of the plasmid was isolated from E. coli transformant and separated on 0.7 % agarose gel electrophoresis. The restriction analysis of pBC15 showed that the plasmid DNA has single site for Bam HI and Eco RI and three sites for Xho I which were compared with 1 Kb DNA and λ Hind III digest molecular markers. Therefore, the size of the plasmid DNA of pBC15 was confirmed to be 23 kb. Curing was carried out by ethidium bromide, acridine orange, novobiocin, sodium dodechyl sulphate and elevated temperature (40 °C). Transformation and curing results suggest that nickel and ampicillin resistance gene was conferred by plasmid DNA. Cadmium resistant gene was present on chromosomal DNA along with the gene for chromium resistance. Lead resistance gene was shown to be present on the chromosomal DNA rather than the plasmid DNA as the cured and uncured cultures remained similar in lead resistance. Therefore, the ability of P. aeruginosa resistant to nickel and ampicillin is plasmid mediated and transferable to other strains whereas cadmium, chromium and lead could be chromosomal encoded. The heavy metal and antibiotic resistances of P. aeruginosa can be used to exploit for clean up industrial wastewater and bioremediation of heavy metal contaminated soil.

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Section: Resultsmentioning

confidence: 97%

Plasmid profile and curing analysis of Pseudomonas aeruginosa as metal resistant

Raja

Selvam

2009

Int. J. Environ. Sci. Technol.

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“…Other results concluded that the frequency of tolerant bacteria increases with an increase of heavy metal concentrations in contaminated soil (Angle et al, 1993;Kunito et al, 2001). The frequency of metal resistance bacteria to metal ions reflect the degree of environmental contamination with these heavy metals and may be directly related to exposure of bacteria to them (Roane and Kellogg, 1996;Hassen et al, 1998;Malik and Jaiswal, 2000). The MICs estimated for Cu, Cd, pb, Zn, Hg, Ni, Co, Cr, As, and Te for bacterial isolates in the contaminated soil as well as in the nonuncontaminated soil were at the levels regarded as those typical for metal-resistant species (Kunito, 1997).…”

Section: Discussionmentioning

confidence: 96%

Isolation, characterization and application of bacterial population from agricultural soil at Sohag Province, Egypt

Bahig¹,

Aly²,

Abdelouahed³

et al. 2008

MJM

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Forty soil samples of agriculture soil were collected from two different sites in Sohag province, Egypt, during hot and cold seasons. Twenty samples were from soil irrigated with canal water (site A) and twenty samples were from soil irrigated with wastewater (site B). This study aimed to compare the incidence of plasmids in bacteria isolated from soil and to investigate the occurrence of metal and antibiotic resistance bacteria, and consequently to select the potential application of these bacteria in bioremediation. The total bacterial count (CFU/gm) in site (B) was higher than that in site (A). Moreover, the CFU values in summer were higher than those values in winter at both sites. for plasmid-possessed bacteria were determined and the highest MICs were 1200 g/mL for lead, 800 g/mL for both Cobalt and Arsenate, 1200 g/mL for Nickel, 1000 g/ml for Copper and less than 600 g/mL for other metals. Bacterial isolates from both sites A and B showed multiple heavy metal resistance. A total of 337 bacterial isolates contained plasmids and the incidence of plasmids was approximately 25-50% higher in bacteria isolated from site (B) than that from site (A). These isolates were resistance to different antibiotics. Approximately, 61% of the bacterial isolates were able to assimilate insecticide, carbaryl, as a sole source of carbon and energy. However, the Citrobacter AA101 showed the best growth on carbaryl.

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“…The concentration of metal pollutants in the environment is usually low excluding in specific areas, which are polluted by various hospitals and industrial wastes. The concentration of heavy metals is very high in ore containing and mining areas (Roane et al, 1996). This has led to growing concern about the consequence of toxic metals as environmental pollutants.…”

Section: Introductionmentioning

confidence: 99%

Metal tolerance analysis of Gram negative bacteria from hospital effluents of Northern India

2017

J App Pharma Sci

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Effluents from different hospitals were analysed to Nickel, Chromium, Cobalt, Copper, Mercury, Cadmium and Zinc resistance among Gram negative bacteria. The resistance among the Gram negative bacterial population varied considerably in different metal and water sampling sites. Gram negative bacteria showed lower metal resistant viable count range 4.01 x10 4 -1.3 x10 3 at 50-100 μg/ml in site-IV as compared to 11.03x105-1.03x10 4 , 12.02x10 5 -1.4x10 3 and 12.33x10 5 -2.7x10 3 in site-I, II and III against all metal tested, respectively. Viable counts of Gram negative bacterial population were recorded higher against Nickel and Zinc from sampling site-III as compared to other sites tested. Lower viable counts of Gram negative bacteria were recorded against Mercury in all sites tested. All the isolates of Gram negative bacteria showed their tolerance level (Minimum inhibitory concentration) in the range of 50-1600μg/ml against all the metal tested. Of 88%, 76% and 86% isolates exhibited their MIC at 50-100 μg/ml against Mercury, Cadmium and Cobalt in all the sites tested, respectively. Maximum 60% and 32% of the isolates demonstrated their MIC at 1200-1600 μg/ml against Cr 2+ and Cu 2+ from the entire site tested, respectively. All Gram negative bacterial isolates also observed multiple resistance patterns (2-7 metal) in different combination of metals. The Multi metal resistance Index (MMR) index ranges were found (0.03-0.71) indicating the high risk of environmental contamination and emergence of metal resistance which may promote the development of resistance to antibiotics among the pathogens.

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Characterization of bacterial communities in heavy metal contaminated soils

Cited by 174 publications

References 20 publications

Plasmid profile and curing analysis of Pseudomonas aeruginosa as metal resistant

Plasmid profile and curing analysis of Pseudomonas aeruginosa as metal resistant

Isolation, characterization and application of bacterial population from agricultural soil at Sohag Province, Egypt

Metal tolerance analysis of Gram negative bacteria from hospital effluents of Northern India

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