Characterization of Bacterial Cell Size and Ploidy using Flow Cytometry and Image Analysis

Durodie, J.; Coleman, Kenneth; Wilkinson, M. J.

doi:10.1007/978-1-4471-2017-9_7

Cited by 12 publications

(13 citation statements)

References 18 publications

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“…In early studies, Steen and coworkers used concentrations exceeding the MIC values for bacteria (302,303) but other authors used lower and even sub-MIC concentrations (87,198). Over the last few years, antimicrobial effects have been studied at clinically achievable concentrations (58,200,237,329,330).…”

Section: Factors Affecting Bacterial and Fungal Susceptibility Testingmentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Alvarez-Barrientos¹,

Arroyo²,

Cantón³

et al. 2000

Clinical Microbiology Reviews

161

112

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Section: Factors Affecting Bacterial and Fungal Susceptibility Testingmentioning

confidence: 99%

Applications of Flow Cytometry to Clinical Microbiology

Alvarez-Barrientos¹,

Arroyo²,

Cantón³

et al. 2000

Clinical Microbiology Reviews

161

112

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“…Although some authors have reported that forward scatter is correlated with cell volume [8,9,10], the lack of correlation between light-scatter and size has also been recently recognized in bacteria [6,7]. This lack of correlation can be explained because light scattering is sensitive to cell size, shape and internal structure and cell envelopes [6,7,16]. E. coli is a rod (a structure very far from the perfect sphere for which the light scattering theory was established and verified), which can result in a wide range of FALS variations, the interpretation of which is very difficult.…”

Section: Fals Variations During Batch Culturementioning

confidence: 99%

“…In the last 15 years flow cytometers have been applied to microorganisms in studies with diverse aims [1][2][3][4][5]. The lack of correlation between light-scatter and size has recently been recognized in bacteria [6,7], although some authors have reported that forward scatter is correlated with cell volume [8,9,10]. The aim of the present study was to compare cell size with FALS in different phases of batch culture of E. coli.…”

Section: Introductionmentioning

confidence: 99%

Variations in flow cytometric forward scatter signals and cell size in batch cultures of Escherichia coli

López-Amorós¹

1994

FEMS Microbiology Letters

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Flow cytometry was used to study the lag, exponential, stationary and death phases of non-fixed cultures of Escherichia coli. Fluctuations in the forward angle scatter signal (FALS) were compared with cell size as measured by scanning electron microscopy at low temperature and image analysis. A correlation between FALS and cell size was not observed, although a correlation (r = -0.8) was obtained between FALS and the age of the culture for the first eleven days of incubation. Marked increases in FALS were observed during the lag phase, which were attributed both to changes in size and changes in structure or chemical composition. The distribution of FALS for all culture phases was asymmetric, and was associated with the cell size distribution.

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“…Although some authors have reported that forward scatter is correlated with cell volume (Robertson and Button 1989;Allman et al 1990;Thorsen et al 1992), the lack of correlation between light-scatter and size has also bccn recognized in bacteria (Allman et al 1993;Durodie et a/. 1993).…”

Section: Nals Variations During Starvatlonmentioning

confidence: 99%

Flow cytometric narrow-angle light scatter and cell size during starvation of Escherichia coli in artificial sea water

Vives-Rego

López-Amorós

Comas

1994

Lett Appl Microbiol

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Flow cytometry was used to study starvation of Escherichia coli in artificial sea water. Flow cytometric narrow‐angle light scatter was compared and assessed in relation to the cell sizes obtained by scanning electron microscopy at low temperature, and by image analysis. A correlation between narrow‐angle light scatter and cell size was not observed, although an acceptable correlation (γ= ‐0.845) between narrow‐angle light scatter and the starvation period was observed. On the other hand, the distribution of narrow‐angle light scatter at any given moment of culture is asymmetric and may be associated with the cell size distribution at the specific moment of starvation.

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Characterization of Bacterial Cell Size and Ploidy using Flow Cytometry and Image Analysis

Cited by 12 publications

References 18 publications

Applications of Flow Cytometry to Clinical Microbiology

Applications of Flow Cytometry to Clinical Microbiology

Variations in flow cytometric forward scatter signals and cell size in batch cultures of Escherichia coli

Flow cytometric narrow-angle light scatter and cell size during starvation of Escherichia coli in artificial sea water

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