Characterization of bacterial biofilm infections with Fourier transform infrared spectroscopy: a review

Chirman, Dylan; Pleshko, Nancy

doi:10.1080/05704928.2020.1864392

Cited by 19 publications

(25 citation statements)

References 159 publications

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“…As such, Figures 2 and 3 present the infrared absorption spectra of the dental biofilm before prophylaxis (stage I), after toothpaste (stage II), and after calcium glycerophosphate mineral complex (stage III), averaged over the groups participating in the study. Analysis of the obtained spectra, based on studies in which IR spectroscopy was used to study oral biological fluids, biofilms, human dental hard tissues, and phosphates relevant to enamel and dentin formation [26,28,29,[41][42][43][44][45][46][47], showed that the experimental IR spectra of biofilms have a typical set of basic vibrations.…”

Section: Resultsmentioning

confidence: 99%

“…The main absorption bands present in all IR spectra of biofilm samples can be associated with the following molecular groups. Analysis of the obtained spectra, based on studies in which IR spectroscopy was used to study oral biological fluids, biofilms, human dental hard tissues, and phosphates relevant to enamel and dentin formation [26,28,29,[41][42][43][44][45][46][47], showed that the experimental IR spectra of biofilms have a typical set of basic vibrations.…”

Section: Resultsmentioning

confidence: 99%

“…Anomeric ring vibrations for tryptophan, tyrosine, and phenyloalanine 900-800 [29,42] The broad vibrational band at 3600-3100 cm −1 corresponds to the N-H bonds of proteins and may also be related to the presence of O-H hydroxyl groups (i.e., water) in the samples [28,29,42]. The group of bands localised in the range of 2950-2750 cm −1 corresponds to vibrations of C-H bonds of various fatty acids and lipids [28,29]. The intensities of these bands in the infrared spectra of biofilms were observed for groups with different cariogenic conditions (Figures 2 and 3).…”

Section: Fingerprint Regionmentioning

confidence: 99%

“…The most convenient and sought-after approach is Fourier transform infrared spectroscopy (FTIR) [ 28 , 29 , 30 , 31 ]. The use of FTIR in biofilm analysis has shown promising results, particularly for the detection and identification of biofilm-forming bacteria [ 28 , 29 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

et al. 2022

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(1) Objectives: This study is the first one to investigate the molecular composition of the dental biofilm during the exogenous and endogenous prophylaxis stages (use of dentifrice/drug) of individuals with different cariogenic conditions using molecular spectroscopy methods. (2) Materials and Methods: The study involved 100 participants (50 males and 50 females), aged 18–25 years with different caries conditions. Biofilm samples were collected from the teeth surface of all participants. The molecular composition of biofilms was investigated using synchrotron infrared microspectroscopy. Changes in the molecular composition were studied through calculation and analysis of ratios between organic and mineral components of biofilm samples. (3) Results: Based on the data obtained by synchrotron FTIR, calculations of organic and mineral component ratios, and statistical analysis of the data, we were able to assess changes occurring in the molecular composition of the dental biofilm. Variations in the phosphate/protein/lipid, phosphate/mineral, and phospholipid/lipid ratios and the presence of statistically significant intra- and inter-group differences in these ratios indicate that the mechanisms of ion adsorption, compounds and complexes arriving from oral fluid into dental biofilm during exo/endogenous prophylaxis, differ for patients in norm and caries development. (4) Conclusions: The conformational environment and charge interaction in the microbiota and the electrostatic state of the biofilm protein network in patients with different cariogenic conditions play an important role. (5) Clinical Significance: Understanding the changes that occur in the molecular composition of the dental biofilm in different oral homeostasis conditions will enable successful transition to a personalised approach in dentistry and high-tech healthcare.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Fingerprint Regionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

et al. 2022

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“…To achieve this goal, we need to determine the mechanism for biofilm formation and growth and the factors involved. This requires appropriate evaluation processes (optical microscopes and electron microscopes [8,9], scanning electron microscopes [10][11][12], confocal laser microscopy [13][14][15][16][17], FTIR [18][19][20][21], and Raman spectroscopy [22][23][24][25]). When we began our investigations about biofilms from the viewpoint of materials science [26][27][28][29][30][31][32][33], we did not have any appropriate and well-known biofilm evaluation processes to use.…”

Section: Introductionmentioning

confidence: 99%

Proposal for Some Affordable Laboratory Biofilm Reactors and Their Critical Evaluations from Practical Viewpoints

et al. 2022

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Biofilms are a result of bacterial activities and are found everywhere. They often form on metal surfaces and on the surfaces of polymeric compounds. Biofilms are sticky and mostly consist of water. They have a strong resistance to antimicrobial agents and can cause serious problems for modern medicine and industry. Biofilms are composed of extracellular polymeric substances (EPS) such as polysaccharides produced from bacterial cells and are dominated by water at the initial stage. In a series of experiments, using Escherichia coli, we developed three types of laboratory biofilm reactors (LBR) to simulate biofilm formation. For the first trial, we used a rotary type of biofilm reactor for stirring. For the next trial, we tried another rotary type of reactor where the circular plate holding specimens was rotated. Finally, a circular laboratory biofilm reactor was used. Biofilms were evaluated by using a crystal violet staining method and by using Raman spectroscopy. Additionally, they were compared to each other from the practical (industrial) viewpoints. The third type was the best to form biofilms in a short period. However, the first and second were better from the viewpoint of “ease of use”. All of these have their own advantages and disadvantages, respectively. Therefore, they should be properly selected and used for specific and appropriate purposes in the future.

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An Entropy-Based Computational Classifier for Positive and Negative Emotions in Voice Signals

Herrera-Ortiz

Yáñez-Casas²,

Hernández-Gómez³

et al. 2022

Communications in Computer and Information Science

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Characterization of bacterial biofilm infections with Fourier transform infrared spectroscopy: a review

Cited by 19 publications

References 159 publications

Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study

Proposal for Some Affordable Laboratory Biofilm Reactors and Their Critical Evaluations from Practical Viewpoints

An Entropy-Based Computational Classifier for Positive and Negative Emotions in Voice Signals

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