Characterization of bacteria associated with omphalitis in chicks

Nasrin, Shamima; Islam, MA; Khatun, Mst. Minara; Akhter, Laila; Sultana, Sharmin

doi:10.3329/bvet.v29i2.14344

Cited by 9 publications

(15 citation statements)

References 6 publications

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“…species in collected samples of chick meconium (49) from hatchery and this findings was very close to the findings of several researchers (Nasrin et al, 2012). These findings also indicate that vertical transmission of Salmonella species occurred to the chick that was similar to the findings reported by several researchers (Cason et al, 1994;Bailey et al, 1994).…”

Section: Resultssupporting

confidence: 90%

Molecular identification and characterization of Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh

Mamun¹,

Kabir²,

Islam³

et al. 2017

Afr. J. Microbiol. Res.

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The aim of this study was to identify and characterize Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh during the period of October 2015 to May 2016. For this purpose a total of 153 samples (35 chick meconium, 49 cloacal swab, 30 poultry carcass, 14 feed, 16 water, 7 transport swab, 2 floor swab) were collected and were subjected to various cultural, biochemical techniques and polymerase chain reaction (PCR). Furthermore, the isolated Salmonella species were characterized by antimicrobial susceptibility testing. Among the samples, 23.53% (n=36) were found to be associated with Salmonella species. The Salmonella species were identified by observing black centered colonies on XLD agar, positive to MR test and negative to VP and Indole test. All isolates of Salmonella species were positive to 16s rRNA gene based PCR (574 bp). Serogrouping of Salmonella species were performed by slide agglutination test using commercial Salmonella specific polyvalent O (A-I) antisera, Salmonella O group B (Factor O: 4, 5, 27) antisera and Salmonella O group D (Factor O: 9, 46) antisera. Among the 36 isolates, 30.56% (n=11) belonged to serogroup B and rest of the isolates 69.44% (n=25) to serogroup D. The most prevalent serogroup identified in this study was serogroup D. The isolated Salmonella species were subjected to antimicrobial susceptibility testing with the aid of disk diffusion method using 8 antimicrobial agents. All isolates of Salmonella species were susceptible to ciprofloxacin, norfloxacin, streptomycin and gentamicin. Out of 36 isolates 100% Salmonella species were resistant to erythromycin and tetracycline. The findings of this study revealed the presence of multidrug resistant Salmonella species in poultry value chains of Gazipur and Tangail districts of Bangladesh that posseses a serious threat to public and poultry health. To the best of our knowledge, this is the first report on the prevalence, serogrouping and antimicrobial resistance patterns of Salmonella species from poultry value chains of selected districts in Bangladesh.

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Section: Resultssupporting

confidence: 90%

Molecular identification and characterization of Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh

Mamun¹,

Kabir²,

Islam³

et al. 2017

Afr. J. Microbiol. Res.

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“…The resistance of E. coli, Salmonella and S. aureus to multiple antimicrobials observed in this study supports the reports of previous works (Khan et al, 2002;Nasrin et al, 2012;Lee et al, 2005;Abadi et al, 2013). This may probably suggest the wide use of these antimicrobials for treatment of bacterial infections in both animals and humans as these bacteria are environmental contaminants from clinical cases.…”

Section: Disc Content (µG)supporting

confidence: 92%

Isolation and characterization of bacteria associated with yolk sac infection (Omphalitis) in chicken from three hatcheries in Bishoftu, Ethiopia

Melese¹,

Esatu²,

Abayneh³

2017

Afr. J. Microbiol. Res.

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A study was conducted from November 2014 to June 2015 at three hatcheries designated as A, D and E in Bishoftu Town, Ethiopia, to isolate and identify bacteria associated with yolk sac infection and to determine antimicrobial susceptibility profile of the predominant isolates. A total of 385 Lohmann and Koekoek breed, 1 to 7 days old chicks suffering from yolk sac infection were examined from three different hatcheries of which 96.1% (N=370) of them were showing unabsorbed yolk sac. All the chicks were necropsied and yolk sac samples were collected for isolation and identification of bacteria followed by testing of the isolates for their susceptibility to 11 antimicrobial agents using disc diffusion method. A total of 323 bacterial isolates were identified, of these Escherichia coli were the most common bacteria (N=116; 35.91%) isolated followed by Salmonella species (N=111; 34.36%) and Staphylococcus aureus (N=96; 29.72%). Significant difference (P<0.001) was noted among the hatcheries on the frequency of isolation of the predominant bacteria species from yolk sac samples with the highest rate of isolation being in hatchery A. All the tested predominant bacterial isolates showed higher susceptibility to Gentamicin, Chloramphenicol, Amikacin (except S. aureus) and Kanamycin but were resistant to Ciprofloxacin, (except S. aureus), Penicillin G, Tetracycline, Sulfamethoxazole and Amoxicillin (except S. aureus). The existence of multi-drug resistant bacteria isolates associated with yolk sac infection suggests that more emphasis should be given towards preventing omphalitis in chicks through improvements of sanitary measures at hatcheries than to use antimicrobials to control infections.

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“…The lamina propria and submucosa consisted of loose connective tissue. The tunica muscularis was strongly thick and constructed of parallel smooth muscle fibers covered by the outer serosa (Nasrin et al, 2012). There were paucity of work studied the histomorphological and histochemical structures of the mallard's stomach and to our knowledge there were no local studies yet.…”

Section: Introductionmentioning

confidence: 99%

“…The cavities converged to form a common duct that lead to the surface through the apex of a small papilla. The surface epithelium on the folds was a simple columnar tissue and each fold had a core of lamina propria to support it (Nasrin et al, 2012). Rossi et al (2005) observed in the gizzard's mucosa of the partridge (Rhynchotus rufescens) low folds lined by simple columnar to cuboidal epithelium.…”

Section: Introductionmentioning

confidence: 99%

Histomorphological and Histochemical Studies of the Stomach of the Mallard (Anas platyrhynchos)

Al-Saffar¹,

Al-Samawy²

2015

Asian J. of Animal Sciences

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The study aimed to find out the histomorphological and histochemical structures of the walls of the stomach parts in mallard. To conduct this investigation, twelve healthy birds were collected from local suppliers at Baghdad province. Later on, birds were subdivided into three equal subdivisions, each of four for gross, histological and histochemical approaches. All birds were anesthetized and their abdomens were dissected, then their stomach were identified and photographed in situ. Organs were grossly described and specimens were immersed either in 10% neutral buffered formalin or in Bouin's solution for histological fixation and then processed for histological and histochemical staining techniques, which were comprised Hematoxylin-eosin, Masson Trichrome, Periodic Acid Schiff (PAS) and PAS-Alcian Blue (AB) (pH 2.5). The gross findings showed that the stomach was consisted of two distinguishable chambers, that were proventriculus and ventriculus. The proventriculus was tubular-shaped organ. The ventriculus appeared fusiform in shape and covered by thin fatty tissue. There was well demarcation between proventriculus and ventriculus. Histologically, the proventriculus mucosa lining was simple columnar epithelium. The submucosal consists of numerous and voluminous deep submucosal glands. The covering of the ventriculus was simple cuboidal. Presence of thick cuticle layer with well-developed muscular stomach. Muscularis mucosa appeared characteristically thick layer of smooth muscle bundles arranged as, inner circular and outer longitudinal layers. The tunica muscularis appeared, as a very thick structure of smooth muscles fibers. Three layers of muscles were distinguished that were thin inner, outer longitudinal and very thick intermediate circular layers.

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Characterization of bacteria associated with omphalitis in chicks

Cited by 9 publications

References 6 publications

Molecular identification and characterization of Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh

Molecular identification and characterization of Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh

Isolation and characterization of bacteria associated with yolk sac infection (Omphalitis) in chicken from three hatcheries in Bishoftu, Ethiopia

Histomorphological and Histochemical Studies of the Stomach of the Mallard (Anas platyrhynchos)

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